Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 555,765 T→G 10.5% S28A (TCG→GCG)  rplN → 50S ribosomal protein L14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947555,7650TG10.5% 159.9 / 10.7 57S28A (TCG→GCG) rplN50S ribosomal protein L14
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base G (4/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

CTGGGCACTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGTTC  >  NC_002947/555618‑555907
                                                                                                                                                   |                                                                                                                                              
cTGGGCACTGGTTGAAGTCCTCGAACGCGCTTTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTc                                                                                                                                               <  5:44125/149‑1 (MQ=255)
   ggCACTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCa                                                                                                                                            <  3:120528/149‑1 (MQ=255)
       cTGGTTGAAGTCCTCGAACGTGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        <  4:71154/149‑1 (MQ=255)
       cTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        <  3:297880/149‑1 (MQ=255)
          gTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTGCGAAccgccg                                                                                                                                      <  1:6453/148‑1 (MQ=255)
             gAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTa                                                                                                                                   <  3:193410/148‑1 (MQ=255)
               aGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGc                                                                                                                                >  8:97831/1‑149 (MQ=255)
                gTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        <  6:188740/140‑1 (MQ=255)
                gTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        >  5:188740/1‑140 (MQ=255)
                          gcgcTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCCATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTg                                                                                                                     <  5:40103/149‑1 (MQ=255)
                               gTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGAcat                                                                                                                 >  8:174799/1‑148 (MQ=255)
                                     gTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGATCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACAtcatca                                                                                                            >  4:103804/1‑147 (MQ=255)
                                          agggctaagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAAc                                                                                                     >  2:118463/1‑149 (MQ=255)
                                          agggctaagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGACAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAAc                                                                                                     >  4:193607/1‑149 (MQ=255)
                                              ctaagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGt                                                                                                  >  7:128179/1‑148 (MQ=255)
                                               taagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTc                                                                                                 >  3:183418/1‑148 (MQ=255)
                                                    gTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAgt                                                                                             >  6:148115/1‑146 (MQ=255)
                                                        gagaAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCaa                                                                                       >  3:68764/1‑149 (MQ=255)
                                                          gaAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAAtt                                                                                     <  7:97831/149‑1 (MQ=255)
                                                            aaTTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTcc                                                                                   <  7:174799/149‑1 (MQ=255)
                                                              ttttATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCtgc                                                                                 <  4:183418/149‑1 (MQ=255)
                                                                 tATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCcgcgc                                                                               <  4:68764/148‑1 (MQ=255)
                                                                     aTTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGt                                                                            >  1:71281/1‑147 (MQ=255)
                                                                      ttCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAg                                                                         >  5:191443/1‑149 (MQ=255)
                                                                         aGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTc                                                                      <  1:118463/149‑1 (MQ=255)
                                                                           aCTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCa                                                                     >  1:217006/1‑148 (MQ=255)
                                                                               aaTCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCaaaaaa                                                                <  8:128179/149‑1 (MQ=255)
                                                                                  ccATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGTTCAAAAAAGGc                                                             >  6:76320/1‑149 (MQ=255)
                                                                                    aTGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGcc                                                            >  5:126844/1‑148 (MQ=255)
                                                                                    aTGCTCGATATGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAg                                                               <  8:121257/145‑1 (MQ=255)
                                                                                    aTGCTCGATATGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAg                                                               >  7:121257/1‑145 (MQ=255)
                                                                                           aTGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGtgatg                                                    <  6:191443/149‑1 (MQ=255)
                                                                                            tgtgGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGtgatga                                                   <  2:217006/149‑1 (MQ=255)
                                                                                               ggCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCg                                                >  5:303017/1‑149 (MQ=255)
                                                                                                  cGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTTGCACCGCCGTTACGCAGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTg                                             >  2:95884/1‑149 (MQ=255)
                                                                                                        cAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGtcgtcg                                       <  3:193607/149‑1 (MQ=255)
                                                                                                           cggcgCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTcgtac                                    <  5:148115/149‑1 (MQ=255)
                                                                                                                 tcgtcgCGTCATGTGCATCAAGGTACTCGGCGGTTTGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCgtacgtac                                <  1:95884/147‑1 (MQ=255)
                                                                                                                       cgTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACg                        <  6:126844/149‑1 (MQ=255)
                                                                                                                           aTGTGCATCAAGGTACACGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGgtgt                    >  7:240185/1‑149 (MQ=255)
                                                                                                                            tgtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGtt                   <  2:71281/149‑1 (MQ=255)
                                                                                                                             gtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCcgcg                                                                                <  8:291625/87‑1 (MQ=255)
                                                                                                                             gtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTcgtac                                    <  3:197094/131‑1 (MQ=255)
                                                                                                                             gtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTcgtac                                    >  4:197094/1‑131 (MQ=255)
                                                                                                                                 aTCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTtcgtcg              >  3:267458/1‑149 (MQ=255)
                                                                                                                                 aTCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTtcgtcg              >  4:93764/1‑149 (MQ=255)
                                                                                                                                     aGGTACACGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCt          <  8:240185/149‑1 (MQ=255)
                                                                                                                                      ggTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACAGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTAGAGCt          >  8:273764/1‑148 (MQ=255)
                                                                                                                                        gtaacggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGAc       >  5:166049/5‑149 (MQ=255)
                                                                                                                                          aacggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACgg     >  3:277103/3‑149 (MQ=255)
                                                                                                                                           tCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTAACCGTCACGGTGTTCGTCGCGCTGACGGt    >  3:237601/1‑149 (MQ=255)
                                                                                                                                           acggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGt    >  4:140563/2‑149 (MQ=255)
                                                                                                                                           acggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGt    >  2:11418/2‑149 (MQ=255)
                                                                                                                                             ggcggTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGt    <  3:103804/147‑1 (MQ=255)
                                                                                                                                             ggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGTTc  <  1:11418/149‑1 (MQ=255)
                                                                                                                                                ggTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCg                                                >  7:286222/1‑100 (MQ=255)
                                                                                                                                                ggTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCg                                                <  8:286222/100‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                              
CTGGGCACTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGTTC  >  NC_002947/555618‑555907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑