Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 555,768 C→A 10.7% H29N (CAC→AAC)  rplN → 50S ribosomal protein L14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947555,7680CA10.7% 158.7 / 9.2 56H29N (CAC→AAC) rplN50S ribosomal protein L14
Reads supporting (aligned to +/- strand):  ref base C (25/25);  new base A (4/2);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

GGCACTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGTTC  >  NC_002947/555621‑555907
                                                                                                                                                   |                                                                                                                                           
ggCACTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCa                                                                                                                                            <  3:120528/149‑1 (MQ=255)
    cTGGTTGAAGTCCTCGAACGTGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        <  4:71154/149‑1 (MQ=255)
    cTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        <  3:297880/149‑1 (MQ=255)
       gTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTGCGAAccgccg                                                                                                                                      <  1:6453/148‑1 (MQ=255)
          gAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTa                                                                                                                                   <  3:193410/148‑1 (MQ=255)
            aGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGc                                                                                                                                >  8:97831/1‑149 (MQ=255)
             gTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        >  5:188740/1‑140 (MQ=255)
             gTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCAccgc                                                                                                                                        <  6:188740/140‑1 (MQ=255)
                       gcgcTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCCATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTg                                                                                                                     <  5:40103/149‑1 (MQ=255)
                            gTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGAcat                                                                                                                 >  8:174799/1‑148 (MQ=255)
                                  gTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGATCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACAtcatca                                                                                                            >  4:103804/1‑147 (MQ=255)
                                       agggctaagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAAc                                                                                                     >  2:118463/1‑149 (MQ=255)
                                       agggctaagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGACAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAAc                                                                                                     >  4:193607/1‑149 (MQ=255)
                                           ctaagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGt                                                                                                  >  7:128179/1‑148 (MQ=255)
                                            taagggTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTc                                                                                                 >  3:183418/1‑148 (MQ=255)
                                                 gTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAgt                                                                                             >  6:148115/1‑146 (MQ=255)
                                                     gagaAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCaa                                                                                       >  3:68764/1‑149 (MQ=255)
                                                       gaAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAAtt                                                                                     <  7:97831/149‑1 (MQ=255)
                                                         aaTTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTcc                                                                                   <  7:174799/149‑1 (MQ=255)
                                                           ttttATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCtgc                                                                                 <  4:183418/149‑1 (MQ=255)
                                                              tATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCcgcgc                                                                               <  4:68764/148‑1 (MQ=255)
                                                                  aTTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGt                                                                            >  1:71281/1‑147 (MQ=255)
                                                                   ttCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAg                                                                         >  5:191443/1‑149 (MQ=255)
                                                                      aGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTc                                                                      <  1:118463/149‑1 (MQ=255)
                                                                        aCTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCa                                                                     >  1:217006/1‑148 (MQ=255)
                                                                            aaTCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCaaaaaa                                                                <  8:128179/149‑1 (MQ=255)
                                                                               ccATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGTTCAAAAAAGGc                                                             >  6:76320/1‑149 (MQ=255)
                                                                                 aTGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGcc                                                            >  5:126844/1‑148 (MQ=255)
                                                                                 aTGCTCGATATGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAg                                                               >  7:121257/1‑145 (MQ=255)
                                                                                 aTGCTCGATATGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAg                                                               <  8:121257/145‑1 (MQ=255)
                                                                                        aTGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGtgatg                                                    <  6:191443/149‑1 (MQ=255)
                                                                                         tgtgGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGtgatga                                                   <  2:217006/149‑1 (MQ=255)
                                                                                            ggCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCg                                                >  5:303017/1‑149 (MQ=255)
                                                                                               cGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTTGCACCGCCGTTACGCAGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTg                                             >  2:95884/1‑149 (MQ=255)
                                                                                                     cAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGtcgtcg                                       <  3:193607/149‑1 (MQ=255)
                                                                                                        cggcgCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTcgtac                                    <  5:148115/149‑1 (MQ=255)
                                                                                                              tcgtcgCGTCATGTGCATCAAGGTACTCGGCGGTTTGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCgtacgtac                                <  1:95884/147‑1 (MQ=255)
                                                                                                                    cgTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACg                        <  6:126844/149‑1 (MQ=255)
                                                                                                                        aTGTGCATCAAGGTACACGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGgtgt                    >  7:240185/1‑149 (MQ=255)
                                                                                                                         tgtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGtt                   <  2:71281/149‑1 (MQ=255)
                                                                                                                          gtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCcgcg                                                                                <  8:291625/87‑1 (MQ=255)
                                                                                                                          gtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTcgtac                                    >  4:197094/1‑131 (MQ=255)
                                                                                                                          gtgCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTcgtac                                    <  3:197094/131‑1 (MQ=255)
                                                                                                                              aTCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTtcgtcg              >  4:93764/1‑149 (MQ=255)
                                                                                                                              aTCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTtcgtcg              >  3:267458/1‑149 (MQ=255)
                                                                                                                                  aGGTACACGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCt          <  8:240185/149‑1 (MQ=255)
                                                                                                                                   ggTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACAGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTAGAGCt          >  8:273764/1‑148 (MQ=255)
                                                                                                                                     gtaacggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGAc       >  5:166049/5‑149 (MQ=255)
                                                                                                                                       aacggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACgg     >  3:277103/3‑149 (MQ=255)
                                                                                                                                        tCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTAACCGTCACGGTGTTCGTCGCGCTGACGGt    >  3:237601/1‑149 (MQ=255)
                                                                                                                                        acggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGt    >  4:140563/2‑149 (MQ=255)
                                                                                                                                        acggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGt    >  2:11418/2‑149 (MQ=255)
                                                                                                                                          ggcggTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGt    <  3:103804/147‑1 (MQ=255)
                                                                                                                                          ggcggTGCGAACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGTTc  <  1:11418/149‑1 (MQ=255)
                                                                                                                                             ggTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCg                                                <  8:286222/100‑1 (MQ=255)
                                                                                                                                             ggTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCg                                                >  7:286222/1‑100 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
GGCACTGGTTGAAGTCCTCGAACGCGCTGTTGAAGTCTAAGGGCTAAGGGTCGGAGAAATTTTATGATTCAGACTCAATCCATGCTCGATGTGGCCGATAACAGCGGCGCTCGTCGCGTCATGTGCATCAAGGTACTCGGCGGTTCGCACCGCCGTTACGCCGGTATCGGTGACATCATCAAAGTAACCGTCAAGGAAGCAATTCCGCGCGGTAAGGTCAAAAAAGGCCAAGTGATGACCGCTGTCGTCGTACGTACCCGTCACGGTGTTCGTCGCGCTGACGGTTC  >  NC_002947/555621‑555907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑