Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 85,096 C→T 14.3% intergenic (+74/+56) aroE → / ← PP_0075 shikimate 5‑dehydrogenase/choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294785,0960CT14.3% 115.2 / 9.9 49intergenic (+74/+56)aroE/PP_0075shikimate 5‑dehydrogenase/choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base C (15/27);  new base T (3/4);  total (18/31)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01

TCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTC  >  NC_002947/84952‑85239
                                                                                                                                                 |                                                                                                                                               
tCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCtgt                                                                                                                                              <  5:166151/148‑1 (MQ=255)
tCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCtgt                                                                                                                                              <  5:124593/148‑1 (MQ=255)
      gtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCg                                                                                                                                       >  5:104323/1‑149 (MQ=255)
       tggcgtgAGGCTGGATACGGCGCCGGTGTTGGATGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACGTACTTTGTGGGAACgg                                                                                                                                      <  4:220408/149‑1 (MQ=21)
        ggcgtgAGGCCGGATACGGCGCCGGTTTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGc                                                                                                                                     <  3:273437/149‑1 (MQ=255)
           gtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACGTACTTTGTGGGAACGGCCtt                                                                                                                                  <  2:75527/149‑1 (MQ=21)
            tgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACGTACTTTGTGGGAACGGCCTTg                                                                                                                                 >  7:80393/1‑149 (MQ=21)
              aGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGc                                                                                                                                <  2:274105/148‑1 (MQ=255)
               ggCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACGTACTTTGTGGGAACGGCCTTGCGt                                                                                                                              <  8:80393/149‑1 (MQ=21)
                gCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGGCGTTCCCGCAAAGTACGTCGTACGCCGCTCCGCTGTGGGAGCGGCCGTGCGtg                                                                                                                             >  5:207252/1‑148 (MQ=39)
                gCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTc                                                                                                                             <  7:122378/149‑1 (MQ=255)
                gCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTc                                                                                                                             >  2:243775/1‑149 (MQ=255)
                            gcCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCCCAAAGTACGTTGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTg                                                                                                                  >  1:123106/1‑148 (MQ=255)
                            gcCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGCCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGc                                                                                                                 <  7:2544/149‑1 (MQ=255)
                                  gtTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTAGTAAGCCCAACCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCa                                                                                                           >  4:112842/1‑149 (MQ=255)
                                   ttGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCa                                                                                                           <  1:243775/148‑1 (MQ=255)
                                     ggCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCGGTGGGAGCGGCCTTGCGTCGCGATGGGc                                                                                                                    >  3:171519/1‑137 (MQ=255)
                                       cTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAccc                                                                                                      <  2:56113/149‑1 (MQ=255)
                                         gAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAcc                                                                                                       >  7:125579/1‑146 (MQ=255)
                                           aCTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGaa                                                                                                  <  7:35256/149‑1 (MQ=255)
                                               cgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACCTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATc                                                                                                <  5:283171/147‑1 (MQ=255)
                                                gcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCa                                                                                             <  6:104323/149‑1 (MQ=255)
                                                    gCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCt                                                                                         >  4:88072/1‑149 (MQ=255)
                                                       acgcgctggGGGCTGAGCGGGCCGGGGCCGCTTGGCGGCCCTATCGCGCCGCACGGCCGTCCCCACAATGTACGGCGTACGCCGATCCGCTGTGGCAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCAt                                                                                      <  8:285958/146‑1 (MQ=38)
                                                           gctcgGGGCGGAGGTGGCCGGGGCCGGTTGGCGGCGCTATCGGGACGCAAGGGCGTTCCCACAAAGTACGTCGTACGCCTATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCt                                                                                  <  5:87380/149‑1 (MQ=38)
                                                                gggCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCTTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAc                                                                             <  3:213553/149‑1 (MQ=255)
                                                                gggCTGAGCTGGCCGGGGCCACTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGGGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAAc                                                                             <  2:167162/149‑1 (MQ=255)
                                                                      aGCTGGCCGGGCCGCTTTGCGTCCCTATCGCCACGCAAGGCCTTTCCCACAACGTACGTCGTACGCCTATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATc                                                                       <  2:229484/148‑1 (MQ=38)
                                                                                    gCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAc                                                         >  4:73637/1‑149 (MQ=255)
                                                                                           ggcCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTcc                                                  <  1:208345/149‑1 (MQ=255)
                                                                                                  tCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCt                                           <  7:115845/149‑1 (MQ=255)
                                                                                                    gcgACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc                                         <  3:73637/149‑1 (MQ=255)
                                                                                                      gACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc                                         <  5:23297/147‑1 (MQ=255)
                                                                                                      gACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc                                         >  6:23297/1‑147 (MQ=255)
                                                                                                       aCGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAAc                                      <  3:112842/149‑1 (MQ=255)
                                                                                                          cacggCCGCCCCCACAACGACCGCCGTCCGCCCAACCTCTGTGGCAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcc                                   <  3:174175/146‑1 (MQ=37)
                                                                                                             ggCCGCTCCCACAGAGGATCGGCGTACGACGTACTTTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAActcctc                                 >  8:72917/1‑148 (MQ=25)
                                                                                                             gcccGTTCCCACAACGACCCTCGTACGCCCACCCACTGTGGGACCGGCGTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCg                                <  3:24058/147‑1 (MQ=255)
                                                                                                               ccGCTCCCACAGAGGATCGGCGTACGACGTACTTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCggg                                                                    >  3:164887/1‑111 (MQ=14)
                                                                                                               ccGCTCCCACAGAGGATCGGCGTACGACGTACTTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCggg                                                                    <  4:164887/111‑1 (MQ=14)
                                                                                                                 gTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTc                                            <  6:93321/133‑1 (MQ=255)
                                                                                                                 gTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTc                                            >  5:93321/1‑133 (MQ=255)
                                                                                                                  ttCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCAc                           <  8:125579/149‑1 (MQ=255)
                                                                                                                  ttCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCAc                           <  3:88072/149‑1 (MQ=255)
                                                                                                                      cacaAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGc                       >  5:6744/1‑149 (MQ=255)
                                                                                                                           aGTACGTCGTACGCAGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCt                  >  4:240483/1‑149 (MQ=255)
                                                                                                                                  tcgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCg            >  5:168237/1‑149 (MQ=255)
                                                                                                                                   cgtACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCACATTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCgg           >  5:2324/1‑149 (MQ=255)
                                                                                                                                            aTCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTc  <  6:6744/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTACGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACCCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTGGCCCGGTGCAAGGTC  >  NC_002947/84952‑85239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: