Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 612,844 G→C 8.4% Y446* (TAC→TAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947612,8440GC 8.4% 170.3 / 4.6 57Y446* (TAC→TAG) dxs1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (23/29);  new base C (2/3);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

CGCTGATCGGCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGT  >  NC_002947/612702‑612986
                                                                                                                                              |                                                                                                                                              
cGCTGATCGGCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGt                                                                                                                                               >  1:180232/1‑144 (MQ=255)
cGCTGATCGGCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGt                                                                                                                                               <  2:180232/144‑1 (MQ=255)
cGCTGATCGGCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGa                                                                                                                                           <  5:141058/148‑1 (MQ=255)
   tGATCGGCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAgg                                                                                                                                       >  1:293207/1‑149 (MQ=255)
        ggCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGt                                                                                                                                   <  4:206088/148‑1 (MQ=255)
         gcgcATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAg                                                                                                                                 >  4:291597/1‑149 (MQ=255)
           gcATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCt                                                                                                                               <  2:143336/149‑1 (MQ=255)
             aTTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGc                                                                                                                             <  3:291597/149‑1 (MQ=255)
             aTTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGc                                                                                                                             >  7:289090/1‑149 (MQ=255)
             aTTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCTGc                                                                                                                             <  5:10835/149‑1 (MQ=255)
               tCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCg                                                                                                                           >  8:64249/1‑149 (MQ=255)
                 gggCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGc                                                                                                                         >  3:7770/1‑149 (MQ=255)
                   gCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGt                                                                                                                       >  3:121444/1‑149 (MQ=255)
                     cGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTgg                                                                                                                     <  4:121444/149‑1 (MQ=255)
                       gTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGt                                                                                                                   >  5:248823/1‑149 (MQ=255)
                                gggTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTc                                                                                                          <  1:257907/149‑1 (MQ=255)
                                   tACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTTCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTc                                                                                                       <  6:248823/149‑1 (MQ=255)
                                       gTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCg                                                                                                   >  3:214916/1‑149 (MQ=255)
                                          cAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGAc                                                                                                 <  2:293207/148‑1 (MQ=255)
                                             cggcccggccGTTCTACACCAGGCCGGTGCTGAGCATCTATACCAGTTCGAACTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGAAGCAGCCCAGCAACGACAGGTCCAACCTGACGGcct                                                                                                                       >  2:128917/1‑121 (MQ=255)
                                             cGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAgg                                                                                             <  4:7770/149‑1 (MQ=255)
                                                       gTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTGATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTGGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCTACCAGGCCGGCGcgat                                                                                   <  3:132976/149‑1 (MQ=255)
                                                       gTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGgcgc                                                                                      <  1:245265/146‑1 (MQ=255)
                                                       gTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGgcgc                                                                                      >  2:245265/1‑146 (MQ=255)
                                                        ttGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGcgatc                                                                                  <  7:64249/149‑1 (MQ=255)
                                                        ttGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGGCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGcgatc                                                                                  <  5:122863/149‑1 (MQ=255)
                                                            aCAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATg                                                                              >  5:128447/1‑149 (MQ=255)
                                                                gTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGAATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCg                                                                           >  2:124748/1‑148 (MQ=255)
                                                                     cGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCTCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAg                                                                      >  3:181281/1‑148 (MQ=255)
                                                                      ggTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCTGGGATGAAGCGAAGGTACGACAGGTCGTAGCTGCCGGCGAGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCa                                                                    >  4:61564/1‑149 (MQ=255)
                                                                             aGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGTGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCg                                                               <  4:214916/147‑1 (MQ=255)
                                                                                aTCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGtt                                                          <  8:289090/149‑1 (MQ=255)
                                                                                   ttGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGAc                                                                                                 <  3:90358/107‑1 (MQ=255)
                                                                                   ttGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGAc                                                                                                 >  4:90358/1‑107 (MQ=255)
                                                                                   ttGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTg                                                       >  6:234910/1‑149 (MQ=255)
                                                                                     gcgcAGTTCGTTCTCGTCGCTTGTGGTGATCCCCAGCATGCCCGGGATGCAGCGCAGGTACGACTGGGCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTg                                                       <  3:233191/147‑1 (MQ=255)
                                                                                      cgcAGTTCGTTCTCGTCGCTGGGGGTCATCTCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCAc                                                    <  4:181281/149‑1 (MQ=255)
                                                                                         aGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAAc                                                                        >  6:9507/1‑126 (MQ=255)
                                                                                         aGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAAc                                                                        <  5:9507/126‑1 (MQ=255)
                                                                                              gttcTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCAcgt                                            >  1:106320/1‑149 (MQ=255)
                                                                                                 ctcGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCTg                                                                                                                              >  4:64461/1‑64 (MQ=37)
                                                                                                 ctcGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCTg                                                                                                                              <  3:64461/64‑1 (MQ=37)
                                                                                                  tcgtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACa                                                                       >  7:59819/1‑118 (MQ=255)
                                                                                                  tcgtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACa                                                                       <  8:59819/118‑1 (MQ=255)
                                                                                                    gtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCAcgtcgt                                         <  1:124748/146‑1 (MQ=255)
                                                                                                    gtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTg                                        <  1:103692/147‑1 (MQ=255)
                                                                                                         tGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGc                                 <  8:85210/149‑1 (MQ=255)
                                                                                                           ggggTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTg                               <  5:234910/149‑1 (MQ=255)
                                                                                                                 atcaCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTa                         <  5:93922/149‑1 (MQ=255)
                                                                                                                    aCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGc                      >  3:61402/1‑149 (MQ=255)
                                                                                                                      cAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCAc                    >  3:170729/1‑149 (MQ=255)
                                                                                                                        gCATGCCCGGGATGCAGCGCAGGTACGTCAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGc                  <  2:239257/149‑1 (MQ=255)
                                                                                                                              ccGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAg             >  6:198187/1‑148 (MQ=255)
                                                                                                                               cGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTgg                              <  7:158324/130‑1 (MQ=255)
                                                                                                                               cGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTgg                              >  8:158324/1‑130 (MQ=255)
                                                                                                                                     gCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTg      <  4:170729/148‑1 (MQ=255)
                                                                                                                                        gcgcAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGt  <  5:198187/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
CGCTGATCGGCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGT  >  NC_002947/612702‑612986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑