Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 612,859 G→C 8.4% S441R (AGC→AGG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947612,8590GC 8.4% 177.8 / 5.1 57S441R (AGC→AGG) dxs1‑deoxyxylulose‑5‑phosphate synthase
Reads supporting (aligned to +/- strand):  ref base G (23/29);  new base C (2/3);  total (25/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGTAGATCGCCACCACCGGCTTG  >  NC_002947/612711‑613006
                                                                                                                                                    |                                                                                                                                                   
gcgcATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAg                                                                                                                                                     >  4:291597/1‑149 (MQ=255)
  gcATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCt                                                                                                                                                   <  2:143336/149‑1 (MQ=255)
    aTTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGc                                                                                                                                                 <  3:291597/149‑1 (MQ=255)
    aTTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGc                                                                                                                                                 >  7:289090/1‑149 (MQ=255)
    aTTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCTGc                                                                                                                                                 <  5:10835/149‑1 (MQ=255)
      tCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCg                                                                                                                                               >  8:64249/1‑149 (MQ=255)
        gggCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGc                                                                                                                                             >  3:7770/1‑149 (MQ=255)
          gCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGt                                                                                                                                           >  3:121444/1‑149 (MQ=255)
            cGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTgg                                                                                                                                         <  4:121444/149‑1 (MQ=255)
              gTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGt                                                                                                                                       >  5:248823/1‑149 (MQ=255)
                       gggTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTc                                                                                                                              <  1:257907/149‑1 (MQ=255)
                          tACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTTCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTc                                                                                                                           <  6:248823/149‑1 (MQ=255)
                              gTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCg                                                                                                                       >  3:214916/1‑149 (MQ=255)
                                 cAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGAc                                                                                                                     <  2:293207/148‑1 (MQ=255)
                                    cggcccggccGTTCTACACCAGGCCGGTGCTGAGCATCTATACCAGTTCGAACTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGAAGCAGCCCAGCAACGACAGGTCCAACCTGACGGcct                                                                                                                                           >  2:128917/1‑121 (MQ=255)
                                    cGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAgg                                                                                                                 <  4:7770/149‑1 (MQ=255)
                                              gTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTGATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTGGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCTACCAGGCCGGCGcgat                                                                                                       <  3:132976/149‑1 (MQ=255)
                                              gTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGgcgc                                                                                                          >  2:245265/1‑146 (MQ=255)
                                              gTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGgcgc                                                                                                          <  1:245265/146‑1 (MQ=255)
                                               ttGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGcgatc                                                                                                      <  7:64249/149‑1 (MQ=255)
                                               ttGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGGCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGcgatc                                                                                                      <  5:122863/149‑1 (MQ=255)
                                                   aCAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATg                                                                                                  >  5:128447/1‑149 (MQ=255)
                                                       gTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGAATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCg                                                                                               >  2:124748/1‑148 (MQ=255)
                                                            cGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCTCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAg                                                                                          >  3:181281/1‑148 (MQ=255)
                                                             ggTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCTGGGATGAAGCGAAGGTACGACAGGTCGTAGCTGCCGGCGAGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCa                                                                                        >  4:61564/1‑149 (MQ=255)
                                                                    aGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGTGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCg                                                                                   <  4:214916/147‑1 (MQ=255)
                                                                       aTCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGtt                                                                              <  8:289090/149‑1 (MQ=255)
                                                                          ttGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGAc                                                                                                                     >  4:90358/1‑107 (MQ=255)
                                                                          ttGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGAc                                                                                                                     <  3:90358/107‑1 (MQ=255)
                                                                          ttGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTg                                                                           >  6:234910/1‑149 (MQ=255)
                                                                            gcgcAGTTCGTTCTCGTCGCTTGTGGTGATCCCCAGCATGCCCGGGATGCAGCGCAGGTACGACTGGGCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTg                                                                           <  3:233191/147‑1 (MQ=255)
                                                                             cgcAGTTCGTTCTCGTCGCTGGGGGTCATCTCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCAc                                                                        <  4:181281/149‑1 (MQ=255)
                                                                                aGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAAc                                                                                            <  5:9507/126‑1 (MQ=255)
                                                                                aGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAAc                                                                                            >  6:9507/1‑126 (MQ=255)
                                                                                     gttcTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCAcgt                                                                >  1:106320/1‑149 (MQ=255)
                                                                                        ctcGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCTg                                                                                                                                                  >  4:64461/1‑64 (MQ=37)
                                                                                        ctcGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGCTACGACCTGTCGTACCTg                                                                                                                                                  <  3:64461/64‑1 (MQ=37)
                                                                                         tcgtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACa                                                                                           <  8:59819/118‑1 (MQ=255)
                                                                                         tcgtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACa                                                                                           >  7:59819/1‑118 (MQ=255)
                                                                                           gtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCAcgtcgt                                                             <  1:124748/146‑1 (MQ=255)
                                                                                           gtcgCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTg                                                            <  1:103692/147‑1 (MQ=255)
                                                                                                tGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGc                                                     <  8:85210/149‑1 (MQ=255)
                                                                                                  ggggTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTg                                                   <  5:234910/149‑1 (MQ=255)
                                                                                                        atcaCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTa                                             <  5:93922/149‑1 (MQ=255)
                                                                                                           aCCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGc                                          >  3:61402/1‑149 (MQ=255)
                                                                                                             cAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCAc                                        >  3:170729/1‑149 (MQ=255)
                                                                                                               gCATGCCCGGGATGCAGCGCAGGTACGTCAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGc                                      <  2:239257/149‑1 (MQ=255)
                                                                                                                     ccGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAg                                 >  6:198187/1‑148 (MQ=255)
                                                                                                                      cGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTgg                                                  <  7:158324/130‑1 (MQ=255)
                                                                                                                      cGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTgg                                                  >  8:158324/1‑130 (MQ=255)
                                                                                                                            gCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTg                          <  4:170729/148‑1 (MQ=255)
                                                                                                                               gcgcAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGt                      <  5:198187/149‑1 (MQ=255)
                                                                                                                                         gACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGTAGATCGccac            <  6:255601/149‑1 (MQ=255)
                                                                                                                                              gTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGt                                              >  5:215168/1‑110 (MQ=255)
                                                                                                                                              gTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGt                                              <  6:215168/110‑1 (MQ=255)
                                                                                                                                              gTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAg                                            <  8:96562/112‑1 (MQ=255)
                                                                                                                                                   aGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCCCGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGTAGATCGCCACCACCGGCTtg  >  6:167225/1‑149 (MQ=255)
                                                                                                                                                   aGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGTAGATCGCCACCACCGGCTtg  >  7:244954/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCGCATTCGGGCCGGTACCACGCGGGTACCGTACAGCGGCCGGGCCGTTGTACAGGTGGCCGGTGCTGAGCATCTTGCGCAGTTCGTTCTCGTCGCTGGGGGTCATCACCAGCATGCCCGGGATGCAGCGCAGGTACGACAGGTCGTAGCTGCCGGCGTGGGTCGGGCCGTCCTCGCCGACCAGGCCGGCGCGATCGATGGCGAACAGCACGTCGAGGTTCTGCACCGCCACGTCGTGGATCAGCTGGTCGTAGGCACGCTGCAGGAAGGTGGAGTAGATCGCCACCACCGGCTTG  >  NC_002947/612711‑613006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑