Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	614,489	A→T	5.9%	L253Q (CTG→CAG)	ispA ←	farnesyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	614,489	0	A	T	5.9%	204.5 / 2.6	68	L253Q (CTG→CAG)	ispA	farnesyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (29/34);  new base T (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

AGGGATAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCATCC  >  NC_002947/614341‑614635                                                                                                                                                      |                                                                                                                                                   aGGGATAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCca                                                                                                                                                    <  2:47540/149‑1 (MQ=255)  gggATAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCcag                                                                                                                                                   <  5:78452/149‑1 (MQ=255)      tAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAgg                                                                                                                                               <  5:38010/149‑1 (MQ=255)      tAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAgg                                                                                                                                               <  1:290590/149‑1 (MQ=255)         gTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTAGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGcc                                                                                                                                            >  3:204638/1‑149 (MQ=255)          tGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCg                                                                                                                                           >  8:105219/1‑149 (MQ=255)               cTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAg                                                                                                                                      <  1:52780/149‑1 (MQ=255)               cTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAg                                                                                                                                      <  5:232981/149‑1 (MQ=255)               cTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAg                                                                                                                                      <  3:106008/149‑1 (MQ=255)                     aGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGt                                                                                                                                >  4:294688/1‑149 (MQ=255)                       ttACGGCGTTCGACAATATAACGCGCCAAGGCCTGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGttt                                                                                                                              <  3:111207/149‑1 (MQ=255)                       ttACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGttt                                                                                                                              >  4:58509/1‑149 (MQ=255)                       ttACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGttt                                                                                                                              <  3:294688/149‑1 (MQ=255)                             cGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCCGCAGGGCCGGGTAGGTCGGTTTGTCAcg                                                                                                                        >  4:103213/1‑149 (MQ=255)                                  gACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCt                                                                                                                    <  5:192872/148‑1 (MQ=255)                                  gACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTa                                                                                                                   >  1:274120/1‑149 (MQ=255)                                   aCAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTAt                                                                                                                  >  1:286817/1‑149 (MQ=255)                                         taACGCGCCAAGGCCCGCAGCGGCTCGGCCTTTTTCGCCGAACCGTTGAAGTGCGGCCAGCGCGTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTg                                                                                                             >  1:196706/1‑149 (MQ=255)                                         taACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGc                                                                                                            <  8:73592/149‑1 (MQ=255)                                           aCGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCa                                                                                                           <  5:300887/148‑1 (MQ=255)                                            cgcgCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCAcc                                                                                                         >  3:233391/1‑149 (MQ=255)                                             gcgcCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCt                                                                                                        >  2:236542/1‑149 (MQ=255)                                                 cAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGc                                                                                                    <  8:69736/149‑1 (MQ=255)                                                     gCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGttt                                                                                                <  2:202770/149‑1 (MQ=255)                                                       ccGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGt                                                                                                                <  7:202803/131‑1 (MQ=255)                                                       ccGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGt                                                                                                                >  8:202803/1‑131 (MQ=255)                                                                cTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTc                                                                                                                           <  2:38083/111‑1 (MQ=255)                                                                cTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTc                                                                                                                           >  1:38083/1‑111 (MQ=255)                                                                   ggCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCa                                                                                   <  4:233391/148‑1 (MQ=255)                                                                          ttCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCg                                                                             <  2:196706/148‑1 (MQ=255)                                                                          ttCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCg                                                                             <  3:288500/148‑1 (MQ=255)                                                                             gCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCtt                                                                          <  2:274120/148‑1 (MQ=255)                                                                              ccGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTc                                                                        >  4:217316/1‑149 (MQ=255)                                                                                 aaCCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCa                                                                                   <  7:74226/135‑1 (MQ=255)                                                                                 aaCCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCa                                                                                   >  8:74226/1‑135 (MQ=255)                                                                                 aaCCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCAc                                                                     >  7:160306/1‑149 (MQ=255)                                                                                    ccTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGt                                                                                       <  2:258290/128‑1 (MQ=255)                                                                                    ccTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGt                                                                                       >  1:258290/1‑128 (MQ=255)                                                                                    ccTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGt                                                                   >  2:189966/1‑148 (MQ=255)                                                                                      ttGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTc                                                                  >  1:100334/1‑147 (MQ=255)                                                                                                 ccAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTcc                                                     <  2:100334/149‑1 (MQ=255)                                                                                                 ccAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTcc                                                     >  7:153199/1‑149 (MQ=255)                                                                                                            tCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGaa                                          <  8:47920/149‑1 (MQ=255)                                                                                                                gcAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGcc                                      <  2:286817/149‑1 (MQ=255)                                                                                                                       gATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCaa                               >  5:281691/1‑149 (MQ=255)                                                                                                                             aTAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCt                         >  7:86021/1‑149 (MQ=255)                                                                                                                             aTAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCt                         >  1:80564/1‑149 (MQ=255)                                                                                                                              tAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtg                        <  3:217316/149‑1 (MQ=255)                                                                                                                               aGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgt                       >  3:228037/1‑149 (MQ=255)                                                                                                                                ggCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgt                       <  3:112539/148‑1 (MQ=255)                                                                                                                                ggCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgt                       <  2:80564/148‑1 (MQ=255)                                                                                                                                 gCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgtg                      >  6:303212/1‑148 (MQ=255)                                                                                                                                   tttGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCttt                                                                         >  5:52696/1‑95 (MQ=255)                                                                                                                                   tttGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCttt                                                                         <  6:52696/95‑1 (MQ=255)                                                                                                                                      ggcggcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAg                >  2:5314/1‑149 (MQ=255)                                                                                                                                        cggcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGtt              >  2:117354/1‑149 (MQ=255)                                                                                                                                         ggcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGttt             <  1:236542/149‑1 (MQ=255)                                                                                                                                          gcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTg            >  3:163116/1‑149 (MQ=255)                                                                                                                                            tacccgacccTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATaa                 >  8:64826/8‑142 (MQ=255)                                                                                                                                            tacccgacccTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATaa                 <  7:64826/135‑1 (MQ=255)                                                                                                                                              ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          <  3:58509/147‑1 (MQ=255)                                                                                                                                              ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          <  3:208968/147‑1 (MQ=255)                                                                                                                                              ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAg         <  7:105219/148‑1 (MQ=255)                                                                                                                                              ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCAAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAg         <  1:189966/148‑1 (MQ=255)                                                                                                                                               ccggCCCTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          >  6:217212/3‑146 (MQ=255)                                                                                                                                               ccggCCCTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          <  5:217212/144‑1 (MQ=255)                                                                                                                                               cAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAggg       <  8:160306/149‑1 (MQ=255)                                                                                                                                                  gCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCAt    >  3:72201/1‑149 (MQ=255)                                                                                                                                                    ccAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTATCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCAGCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCTTGGCATcc  <  4:204638/149‑1 (MQ=255)                                                                                                                                                    ccAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCATc   >  2:300402/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                   AGGGATAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCATCC  >  NC_002947/614341‑614635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑