Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	614,492	A→T	5.9%	L252Q (CTG→CAG)	ispA ←	farnesyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	614,492	0	A	T	5.9%	209.3 / 2.2	68	L252Q (CTG→CAG)	ispA	farnesyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (30/34);  new base T (2/2);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

TAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCATCC  >  NC_002947/614346‑614635                                                                                                                                                    |                                                                                                                                                tAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAgg                                                                                                                                               <  5:38010/149‑1 (MQ=255) tAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAgg                                                                                                                                               <  1:290590/149‑1 (MQ=255)    gTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTAGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGcc                                                                                                                                            >  3:204638/1‑149 (MQ=255)     tGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCg                                                                                                                                           >  8:105219/1‑149 (MQ=255)          cTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAg                                                                                                                                      <  1:52780/149‑1 (MQ=255)          cTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAg                                                                                                                                      <  5:232981/149‑1 (MQ=255)          cTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAg                                                                                                                                      <  3:106008/149‑1 (MQ=255)                aGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGt                                                                                                                                >  4:294688/1‑149 (MQ=255)                  ttACGGCGTTCGACAATATAACGCGCCAAGGCCTGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGttt                                                                                                                              <  3:111207/149‑1 (MQ=255)                  ttACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGttt                                                                                                                              <  3:294688/149‑1 (MQ=255)                  ttACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGttt                                                                                                                              >  4:58509/1‑149 (MQ=255)                        cGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCCGCAGGGCCGGGTAGGTCGGTTTGTCAcg                                                                                                                        >  4:103213/1‑149 (MQ=255)                             gACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCt                                                                                                                    <  5:192872/148‑1 (MQ=255)                             gACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTa                                                                                                                   >  1:274120/1‑149 (MQ=255)                              aCAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTAt                                                                                                                  >  1:286817/1‑149 (MQ=255)                                    taACGCGCCAAGGCCCGCAGCGGCTCGGCCTTTTTCGCCGAACCGTTGAAGTGCGGCCAGCGCGTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTg                                                                                                             >  1:196706/1‑149 (MQ=255)                                    taACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGc                                                                                                            <  8:73592/149‑1 (MQ=255)                                      aCGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCa                                                                                                           <  5:300887/148‑1 (MQ=255)                                       cgcgCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCAcc                                                                                                         >  3:233391/1‑149 (MQ=255)                                        gcgcCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCt                                                                                                        >  2:236542/1‑149 (MQ=255)                                            cAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGc                                                                                                    <  8:69736/149‑1 (MQ=255)                                                gCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGttt                                                                                                <  2:202770/149‑1 (MQ=255)                                                  ccGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGt                                                                                                                <  7:202803/131‑1 (MQ=255)                                                  ccGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGt                                                                                                                >  8:202803/1‑131 (MQ=255)                                                           cTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTc                                                                                                                           <  2:38083/111‑1 (MQ=255)                                                           cTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTc                                                                                                                           >  1:38083/1‑111 (MQ=255)                                                              ggCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCa                                                                                   <  4:233391/148‑1 (MQ=255)                                                                     ttCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCg                                                                             <  2:196706/148‑1 (MQ=255)                                                                     ttCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCg                                                                             <  3:288500/148‑1 (MQ=255)                                                                        gCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCtt                                                                          <  2:274120/148‑1 (MQ=255)                                                                         ccGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTc                                                                        >  4:217316/1‑149 (MQ=255)                                                                            aaCCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCa                                                                                   <  7:74226/135‑1 (MQ=255)                                                                            aaCCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCa                                                                                   >  8:74226/1‑135 (MQ=255)                                                                            aaCCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCAc                                                                     >  7:160306/1‑149 (MQ=255)                                                                               ccTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGt                                                                                       >  1:258290/1‑128 (MQ=255)                                                                               ccTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGt                                                                                       <  2:258290/128‑1 (MQ=255)                                                                               ccTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGt                                                                   >  2:189966/1‑148 (MQ=255)                                                                                 ttGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTc                                                                  >  1:100334/1‑147 (MQ=255)                                                                                            ccAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTcc                                                     <  2:100334/149‑1 (MQ=255)                                                                                            ccAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTcc                                                     >  7:153199/1‑149 (MQ=255)                                                                                                       tCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGaa                                          <  8:47920/149‑1 (MQ=255)                                                                                                           gcAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGcc                                      <  2:286817/149‑1 (MQ=255)                                                                                                                  gATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCaa                               >  5:281691/1‑149 (MQ=255)                                                                                                                        aTAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCt                         >  1:80564/1‑149 (MQ=255)                                                                                                                        aTAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCt                         >  7:86021/1‑149 (MQ=255)                                                                                                                         tAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtg                        <  3:217316/149‑1 (MQ=255)                                                                                                                          aGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgt                       >  3:228037/1‑149 (MQ=255)                                                                                                                           ggCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgt                       <  2:80564/148‑1 (MQ=255)                                                                                                                           ggCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgt                       <  3:112539/148‑1 (MQ=255)                                                                                                                            gCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCtgtg                      >  6:303212/1‑148 (MQ=255)                                                                                                                              tttGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCttt                                                                         >  5:52696/1‑95 (MQ=255)                                                                                                                              tttGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCttt                                                                         <  6:52696/95‑1 (MQ=255)                                                                                                                                 ggcggcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAg                >  2:5314/1‑149 (MQ=255)                                                                                                                                   cggcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGtt              >  2:117354/1‑149 (MQ=255)                                                                                                                                    ggcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGttt             <  1:236542/149‑1 (MQ=255)                                                                                                                                     gcTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTg            >  3:163116/1‑149 (MQ=255)                                                                                                                                       tacccgacccTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATaa                 <  7:64826/135‑1 (MQ=255)                                                                                                                                       tacccgacccTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATaa                 >  8:64826/8‑142 (MQ=255)                                                                                                                                         ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          <  3:208968/147‑1 (MQ=255)                                                                                                                                         ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          <  3:58509/147‑1 (MQ=255)                                                                                                                                         ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAg         <  7:105219/148‑1 (MQ=255)                                                                                                                                         ccAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCAAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAg         <  1:189966/148‑1 (MQ=255)                                                                                                                                          ccggCCCTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          <  5:217212/144‑1 (MQ=255)                                                                                                                                          ccggCCCTGCTGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCa          >  6:217212/3‑146 (MQ=255)                                                                                                                                          cAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAggg       <  8:160306/149‑1 (MQ=255)                                                                                                                                             gCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCAt    >  3:72201/1‑149 (MQ=255)                                                                                                                                               ccAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTATCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCAGCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCTTGGCATcc  <  4:204638/149‑1 (MQ=255)                                                                                                                                               ccAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCATc   >  2:300402/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                TAGGTGAAGCTGTTTAGTTACGGCGTTCGACAATATAACGCGCCAAGGCCCGCAGCGGCTCGGCC‑TTTTCGCCGAACCCTTGCAGTGCGGCCAGCGCCTGGTCGCGCAGTTCGATTGCATAGGCTTTGGCGGCTTCCAGGCCCAGCAGGGCCGGGTAGGTCGGTTTGTCACGCGCTATGTCTGCACCTTGGCGTTTGCCCAAGGTAGCAGTGTCGCTTTCCACGTCGAGGATGTCGTCCTGCACCTGGAATGCCAGGCCAATGGCCTGTGCATAAGTTTGCAGGGCATCC  >  NC_002947/614346‑614635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑