Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	616,451	T→A	6.9%	noncoding (71/142 nt)	PP_mr11 ←	FMN regulatory region

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	616,451	0	T	A	6.9%	216.5 / 5.3	73	noncoding (71/142 nt)	PP_mr11	FMN regulatory region
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base A (3/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

AAACGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAG  >  NC_002947/616303‑616593                                                                                                                                                     |                                                                                                                                               aaaCGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACAt                                                                                                                                                >  4:128001/1‑149 (MQ=255)          tcGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGAAGCCTACTTTCATCCGGACTATGACCTTCGGCTCGGGAGTTGCAACAGATCTGCTGACCCCCGACATGGTCGGGgc                                                                                                                                       >  5:177561/1‑149 (MQ=255)                 tGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCAAGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTcgcg                                                                                                                                >  7:196215/1‑149 (MQ=255)                   aTCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGTCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCgg                                                                                                                               <  4:225131/148‑1 (MQ=255)                   aTCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCggg                                                                                                                              >  8:83387/1‑149 (MQ=255)                   aTCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCggg                                                                                                                              >  1:217627/1‑149 (MQ=255)                   aTCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCgg                                                                                                                               <  6:52667/148‑1 (MQ=255)                           aCGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCtt                                                                                                                      >  3:84989/1‑149 (MQ=255)                            cggctctGGTCGAAAAAAATCCGCGCGCATGTGCGCCCGGATGGCCACTGCATGACGCCTTCTTTCATCCGGAATATGACCGTCGGCTCTGGAGTTGCACCATATCTGCTGACCCCCTACATGGTCTGGGCTCTCTCGGGCTCATCttt                                                                                                                     <  4:116568/146‑1 (MQ=255)                             gTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTc                                                                                                                    <  2:217627/149‑1 (MQ=255)                               ctcGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCggg                                                                                                                                         >  1:156947/1‑126 (MQ=255)                               ctcGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCggg                                                                                                                                         <  2:156947/126‑1 (MQ=255)                                  ggggCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGa                                                                                                                  <  8:196215/146‑1 (MQ=255)                                       gAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTAccgc                                                                                                          >  4:297757/1‑149 (MQ=255)                                       gAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTAccgc                                                                                                          >  2:101226/1‑149 (MQ=255)                                        aaCAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTAccgcc                                                                                                         >  5:266447/1‑149 (MQ=255)                                             aaTGCGCGCGCAAGGGCGCCCGTATGGCCCCTGCAGGACGCCTTCTTTCATCCGGACTATGACCTTCGGCTCTGGATTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTgg                                                                                                    <  6:177561/149‑1 (MQ=255)                                                 cgcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGa                                                                                                 <  3:128001/148‑1 (MQ=255)                                                 cgcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGa                                                                                                 <  1:101226/148‑1 (MQ=255)                                                  gcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggg                                                                                                                                        <  7:275510/108‑1 (MQ=255)                                                  gcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACCATGTCgggg                                                                                                                                        >  8:275510/1‑108 (MQ=255)                                                  gcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGAttt                                                                                                               >  8:192985/1‑133 (MQ=255)                                                  gcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGt                                                                                                      <  1:267824/142‑1 (MQ=255)                                                  gcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGt                                                                                                      >  2:267824/1‑142 (MQ=255)                                                  gcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGAc                                                                                                <  4:216874/148‑1 (MQ=255)                                                  gcgcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACt                                                                                               >  3:216874/1‑149 (MQ=255)                                                    gcgcAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTa                                                                                             <  3:194627/149‑1 (MQ=255)                                                        aaGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACAcc                                                                                         >  1:241236/1‑149 (MQ=255)                                                                ccGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCt                                                                                  >  4:149736/1‑148 (MQ=255)                                                                  ggATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                               <  7:83387/149‑1 (MQ=255)                                                                  ggATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                               <  4:84989/149‑1 (MQ=255)                                                                   gATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTg                                                                                 >  6:107912/1‑146 (MQ=255)                                                                   gATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAg                                                                              <  2:241236/149‑1 (MQ=255)                                                                              cATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                               >  8:126294/1‑137 (MQ=255)                                                                              cATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGaa                                                                               <  7:126294/137‑1 (MQ=255)                                                                              cATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAg                                                                    >  3:75955/1‑148 (MQ=255)                                                                               aTGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCg                                                                                                                   >  5:40417/1‑100 (MQ=255)                                                                               aTGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCg                                                                                                                   <  6:40417/100‑1 (MQ=255)                                                                                tGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCa                                                                     <  2:257328/145‑1 (MQ=255)                                                                                tGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTAACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCa                                                                     >  1:257328/1‑145 (MQ=255)                                                                                   cGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACa                                                              <  6:266447/149‑1 (MQ=255)                                                                                      cttcttTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGca                                                           >  6:306070/1‑149 (MQ=255)                                                                                      cttcttTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACACCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGc                                                            >  3:82975/1‑148 (MQ=255)                                                                                              aTCCGGACTCTGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCa                                                   >  1:135262/1‑149 (MQ=255)                                                                                              aTCCGGACTCTGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCa                                                   <  2:135262/149‑1 (MQ=255)                                                                                               tCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACAGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAc                                                  >  7:51557/1‑149 (MQ=255)                                                                                                ccGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAcc                                                 >  8:224681/1‑149 (MQ=255)                                                                                                ccGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACAGGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAcc                                                 >  1:80369/1‑149 (MQ=255)                                                                                                 cGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAccc                                                <  2:80369/149‑1 (MQ=255)                                                                                                 cGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCAccc                                                <  4:75955/149‑1 (MQ=255)                                                                                                      tATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTg                                           <  8:51557/149‑1 (MQ=255)                                                                                                          tccGTCGGCTCTGGAGTTGCTCGAGATCTGCTGAGCCCCGACATGGTCGGGGCGGTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTg                                                      <  6:63574/133‑1 (MQ=255)                                                                                                          cccGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTg                                                      >  5:63574/2‑134 (MQ=255)                                                                                                          aCCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCaa                                       <  7:224681/149‑1 (MQ=255)                                                                                                          aCCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCa                                        <  5:107912/148‑1 (MQ=255)                                                                                                              tCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCcg                                   >  8:188814/1‑149 (MQ=255)                                                                                                                       gAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTcta                           <  7:188814/148‑1 (MQ=255)                                                                                                                       gAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTc                             <  6:72237/146‑1 (MQ=255)                                                                                                                            gCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCt                     >  7:70815/1‑149 (MQ=255)                                                                                                                             cACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTc                             >  8:52827/1‑140 (MQ=255)                                                                                                                             cACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTc                             <  7:52827/140‑1 (MQ=255)                                                                                                                               ccAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCg                  >  1:145249/1‑149 (MQ=255)                                                                                                                                cAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACGGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCgg                 <  4:82975/149‑1 (MQ=255)                                                                                                                                cAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGcc                                                                                    <  3:93304/82‑1 (MQ=255)                                                                                                                                cAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGcc                                                                                    >  4:93304/1‑82 (MQ=255)                                                                                                                                cAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCgg                 >  4:84150/1‑149 (MQ=255)                                                                                                                                 aGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGc                <  8:70815/149‑1 (MQ=255)                                                                                                                                      tgctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGAc           >  8:93743/1‑149 (MQ=255)                                                                                                                                        ctgACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCga              <  3:149736/144‑1 (MQ=255)                                                                                                                                           cgccccGACCATGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTg        >  2:105509/3‑147 (MQ=255)                                                                                                                                            gccccGACCATGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGc                >  3:7441/2‑138 (MQ=255)                                                                                                                                            gccccGACCATGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGc                <  4:7441/137‑1 (MQ=255)                                                                                                                                             ccccGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTgcag     >  5:222502/1‑148 (MQ=255)                                                                                                                                                cGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGcagcag  >  7:264936/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                               AAACGCTCCTCGTGAATGATCGATGAACGTCTCGGGGCGAACAAAATGCGCGCGCAAGGGCGCCCGGATGGCCCCTGCATGACGCCTTCTTTCATCCGGACTATGACCGTCGGCTCTGGAGTTGCACCAGATCTGCTGACCCCCGACATGGTCGGGGCGCTCGCGGGCTCATCTTTCGATTTACCGCCGGTGGGGACTTACACCCCGCCCTGAAGACCGACCCAGCATACAGCACAGTGCCACCCCAGTGGCAACCCGCGGTTCTACTACCTTCGGCGAGACTTGCAGCAG  >  NC_002947/616303‑616593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑