Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 660,941 A→T 7.8% intergenic (+73/‑94) speA → / → PP_0568 biosynthetic arginine decarboxylase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947660,9410AT 7.8% 143.5 / 3.3 51intergenic (+73/‑94)speA/PP_0568biosynthetic arginine decarboxylase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (22/25);  new base T (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

GTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCGGTCGAGTGTCTGAATCCGG  >  NC_002947/660801‑661087
                                                                                                                                            |                                                                                                                                                  
gTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCa                                                                                                                                            >  7:224955/1‑149 (MQ=255)
  gAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCAc                                                                                                                                           >  3:178987/1‑148 (MQ=255)
       gACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCTTCGCCCTCatatc                                                                                                                                        >  5:271227/1‑142 (MQ=255)
       gACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCTTCGCCCTCatatc                                                                                                                                        <  6:271227/146‑5 (MQ=255)
       gACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCTGCGt                                                                                                                                     <  3:257799/149‑1 (MQ=255)
       gACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGt                                                                                                                                     <  6:211/149‑1 (MQ=255)
         cccAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTca                                                                                                                                   <  6:31535/149‑1 (MQ=255)
                tCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCg                                                                                                                             <  4:53168/148‑1 (MQ=255)
                    gATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTaa                                                                                                                         <  8:224955/148‑1 (MQ=255)
                         cTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATcc                                                                                                                   <  1:60328/149‑1 (MQ=255)
                            gcgcCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGc                                                                                                                >  5:232171/1‑149 (MQ=255)
                                           ccGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCAc                                                                                                 >  5:277554/1‑149 (MQ=255)
                                           ccGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCAc                                                                                                 <  4:178987/149‑1 (MQ=255)
                                              gTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCaa                                                                                              >  8:43073/1‑149 (MQ=255)
                                                    gTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGACCCTGGTCCATCGCACCAATGCCg                                                                                         <  7:43073/148‑1 (MQ=255)
                                                            cgtcgtAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCTTCGCCCTCatatc                                                                                                                                        >  6:305114/1‑89 (MQ=38)
                                                            cgtcgtAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCTTCGCCCTCatatc                                                                                                                                        <  5:305114/93‑5 (MQ=38)
                                                             gtcgtAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGa                                                                               <  5:28867/149‑1 (MQ=255)
                                                                 tAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCttt                                                                                 >  4:97242/1‑143 (MQ=255)
                                                                 tAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCttt                                                                                 <  3:97242/143‑1 (MQ=255)
                                                                 tAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATg                                                                            >  3:231442/1‑148 (MQ=255)
                                                                    ttGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAAt                                                                        <  6:232171/149‑1 (MQ=255)
                                                                       gcaaggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCCCTACCTTTGAATGCAATGta                                                                     >  7:255618/5‑148 (MQ=25)
                                                                          gggCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCa                                                                   >  4:1242/1‑148 (MQ=255)
                                                                           ggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGACCTTGTCCATCGCACCAAAGCCGCTACCTATGAATGCAATCTTCa                                                                   >  7:148599/1‑147 (MQ=25)
                                                                               gCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCTGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAaggag                                                             >  8:10115/1‑149 (MQ=255)
                                                                                    tGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGa                                                        >  4:141931/1‑149 (MQ=255)
                                                                                      cAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGata                                                      >  6:109727/1‑149 (MQ=255)
                                                                                      cAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGa                                                        <  6:277554/147‑1 (MQ=255)
                                                                                       aaaaGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCTGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGatat                                                     <  7:10115/149‑1 (MQ=255)
                                                                                       aaaaGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGatat                                                     <  3:141931/149‑1 (MQ=255)
                                                                                           gCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCaa                                                 >  7:159962/1‑149 (MQ=255)
                                                                                                    gcCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGat                                                       >  7:109180/1‑134 (MQ=255)
                                                                                                    gcCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGat                                                       <  8:109180/134‑1 (MQ=255)
                                                                                                      cTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTc                                      <  8:159962/149‑1 (MQ=255)
                                                                                                       ttttGTTGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCa                                                                          <  4:56020/112‑1 (MQ=255)
                                                                                                       ttttGTTGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCa                                                                          >  3:56020/1‑112 (MQ=255)
                                                                                                        tttGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTcc                                     >  5:35625/1‑148 (MQ=255)
                                                                                                          tgtgGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCa                                   <  3:1242/148‑1 (MQ=255)
                                                                                                            tgGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATc                                 <  2:159085/148‑1 (MQ=255)
                                                                                                            tgGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATc                                 >  1:159085/1‑148 (MQ=255)
                                                                                                            tgGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATc                                 <  4:231442/148‑1 (MQ=255)
                                                                                                             gggAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGt                               >  6:65024/1‑149 (MQ=255)
                                                                                                                     ccgtGCGGCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTgg                       <  6:26724/146‑1 (MQ=255)
                                                                                                                       tagCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCg                     >  1:240339/3‑149 (MQ=255)
                                                                                                                            tCGCTATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCGAGGCTCCCATCGTTTACGTGGCGGTCGa                <  6:4327/149‑1 (MQ=255)
                                                                                                                            tCGCGATATGAGGTCGAAGCCCTCACCAGCTTCACTACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCGGTCGa                <  8:255618/149‑1 (MQ=255)
                                                                                                                                  tatGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCGGTCGAGTGTCt          <  2:186664/149‑1 (MQ=255)
                                                                                                                                    tGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCGGTCGa                <  6:228381/141‑1 (MQ=255)
                                                                                                                                    tGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCGGTCGa                >  5:228381/1‑141 (MQ=255)
                                                                                                                                          cGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCGGTCGAGTGTCTGAATCCgg  >  1:33205/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGGCTCCCATCGTTTACGTGGCGGTCGAGTGTCTGAATCCGG  >  NC_002947/660801‑661087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑