| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 718,457 | C→G | 9.8% | intergenic (+27/+50) | thiO → / ← PP_0613 | FAD‑dependent glycine/D‑amino acid oxidase/amidase family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 718,457 | 0 | C | G | 9.8% | 98.5 / 5.0 | 47 | intergenic (+27/+50) | thiO/PP_0613 | FAD‑dependent glycine/D‑amino acid oxidase/amidase family protein |
| Reads supporting (aligned to +/- strand): ref base C (23/19); new base G (2/2); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-02 | |||||||||||
ATTATCGCAATGGGCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCAGCAAAGCGCCCGGC > NC_002947/718312‑718602 | aTTATCGCAATGGGCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCAGGTAAACCCGCTCCTAc > 3:167338/1‑149 (MQ=255) aTCGCAATGGGCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTTAAACCGCGCCGAagcg > 1:286545/1‑149 (MQ=255) tCGCAATGGGCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATGCCGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTAcgc < 1:238548/148‑1 (MQ=255) cAATGGGCTGGTGCTGGCGCCGGCTTCGTGGCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCg > 1:25131/1‑149 (MQ=255) aaTGGGCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCgg < 5:90960/149‑1 (MQ=255) aaTGGGCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCgg > 1:59971/1‑149 (MQ=255) ggCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGtt < 2:59971/148‑1 (MQ=255) ggCTGGTGCTGGCGCCGGCTGCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGtt < 5:266900/148‑1 (MQ=255) cTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCGCGTAGGAGCGGGTTTAc < 7:236197/149‑1 (MQ=39) gTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCGCGTAGGAGCGGGTTTAcc > 5:129350/1‑147 (MQ=39) gTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCGCGTAGGAGCGGGTTTAcc < 6:129350/147‑1 (MQ=39) gcgcCGGCTTCGGGCCTGCTGTTTACCGTTTTCCTTCCCGGGGCCGAGCGTATAAGCGCCCCGGCGCCTTATG‑CCCCAGACGGGGGTTTGGGCTGAGGACCTCTTCGCGGTTAAACCCGCTCCAACGCGAGCGGGTTTACCCGCG‑AAAg < 2:255381/149‑1 (MQ=255) gcCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAAACGACACGACACGAGGGGGTTTTCCCGAG‑aaaacg > 8:289717/1‑146 (MQ=255) gTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCAGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGaa < 4:167338/148‑1 (MQ=255) tGCCTGCTTTTTACCGATTTGCTGCCCGGGGCCGCGCGTATTAGCGACCCGGCGCGGTATG‑CGCCAGATGGGCGTTTGGGCTGAGCACCTCTTCGCGGTTAACCCCGCTCCTACGCGCGCGGTTTTACCCGCG‑AAAGGGCCGCAGAAGc < 2:286545/149‑1 (MQ=255) gCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAACCGCTCCTAAGCGAGCGGGTTTAACCGCG‑AAAGGGCCGCAGAAGc > 7:172468/1‑148 (MQ=255) ttACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAACCGGTCCTACGCGAGCGGGTTTTCCCGCG‑CAACGGGCCCCGAAGCCAATACAggt > 5:299278/1‑147 (MQ=255) ttACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAACCCCTCCTACGCGCGCGGGTTTTACCGCG‑AAAGGGCCGCAGAAGCCAATACAGAt > 7:5518/1‑149 (MQ=255) tACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACATCTTCGCGGGTAAAACCGCGCCTACGCGGGCCGGGTTTCCCGCG‑AAAGTGCCGCAGAACCCAATAAAGAtt > 7:145098/1‑149 (MQ=255) ggATTTGCTGACAGGGGCCGAGCCTATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGa < 2:223579/131‑1 (MQ=255) aTTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAACCCCAACAAACCGAGCGGGTTAAACCCCG‑AAACGGCCCTATAAGACAATAAAGAtttactg > 6:167922/1‑145 (MQ=255) cTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTTAAACCGCGACGACGCGAGCGGGGTTTACCGGG‑AAAGGGGCGCAGAAGCCAATAAAAATTTGAGGCAGt > 4:258153/1‑148 (MQ=255) cAGGGTCCGAGCCGATTATCTACCGGGGGCCGTATG‑CGCAAGATGGTGGTTTGGGGTTAGGACCTCTTCTCGGGTAAACCCGCTCCTACGCTTGGGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCg < 8:172468/149‑1 (MQ=37) gggCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAACCCCGCCAAAGCGAGCGGGTTTTCCCGCG‑AAAGGGCCCCCGAAGCCAATAAAAATTTAAGCCAGTAATAGGTg > 2:208743/1‑149 (MQ=255) gaTTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTg > 1:29273/1‑149 (MQ=255) cccGGCGCGGTATG‑CGCCTGAAGGGGGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGTGCGGTTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCgg < 8:5518/149‑1 (MQ=39) gcCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGTAAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAAtg < 3:258153/149‑1 (MQ=255) ccGTATG‑CGCCAGACGGGCGTTTGGGCTGAGCACCTCATCGCGGGAAAACCCGCCCCAACGCGCGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCaa < 5:9950/98‑1 (MQ=37) gTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAACCCCGGCAAAACGAGCGGGGGTTACCGCG‑AAAAGGCCCCAGAAACCAAAAAAAATTTAAGGAAGGAATAGGTGCTTTCAGGTGGCCGCGGGGATAAtta > 8:290266/1‑145 (MQ=255) gTAAG‑CGCCAGAACGGAGTTTCGGCTGAGGACCTCTTCGCGGGTAAAACCACACCAACACCACCCGGGTTACAAGCG‑AAACGGCCGCAGAAGCCAAAACAGAAATCAACCAGAAATCAATACTTTCATGTTCCGACTAGGATTAtgctg > 8:131978/1‑149 (MQ=255) aGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTTAAACCGCTACAAAGCGAGCGGGTTTACCCGCC‑AAAGGGGCGGAGAAGCCAAAACAGa > 4:175835/1‑90 (MQ=255) aGGGCGTTTGGGCTGAGGACCTCTTCGCGGGAAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGa < 3:175835/90‑1 (MQ=255) gggCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTg < 8:242458/149‑1 (MQ=255) gggCGTTGGGGCGGCGCCCGTCTTCGCGGGTAAACCCGCTCCTACGCCAGGGGTTTTACCCGCG‑AAAGGGCCGTAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTg < 5:167922/149‑1 (MQ=255) gCGTTTGGGCTGAGGACCTCTTCGCGGGTAAAACCCCACCAACGCGAGCGGGGTTTCCCCCGCAAAGGGCC‑CAGAAACCCAAAAAGAATTAAGCCACTAATACGTTCTTTCAAGTGGCGGCGGGGATAAAACAGGTCAAACAccgatg > 4:279323/1‑144 (MQ=255) cTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCgcag < 2:29273/149‑1 (MQ=255) ccTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTAACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGc < 1:208743/149‑1 (MQ=255) ccTCTTCGCGGGTAAACCCGCGCCTACGCGTGCGGTTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTg < 7:131978/148‑1 (MQ=255) tttCGCGGGTAAACCCGCTCGCGTAGGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCtca > 6:10614/2‑149 (MQ=38) ttCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAAAACAGATTTCAGCCAGTAATCGGTGCTTGCACGTGCCGGCACGGATAATGCTGCTCATACACCTTGCACCCCCGCACCACTTGCtca > 4:260497/1‑148 (MQ=39) gcgGGTAAACCCTCTCTTACGCGTGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCtcatca < 7:289717/148‑1 (MQ=255) aaaCCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCAGCAAAgc > 2:274518/1‑149 (MQ=255) aCCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCTGCAAAgcgc > 3:163783/1‑149 (MQ=255) aCCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCAGCACAgcgc > 4:184557/1‑149 (MQ=255) ccGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCAGCAAAGCGccc > 3:226565/1‑149 (MQ=255) cGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCAGCAAAGCGCCCg < 1:274518/149‑1 (MQ=255) cTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCAGCAAAGCGCCCGGc > 3:3704/1‑149 (MQ=255) | ATTATCGCAATGGGCTGGTGCTGGCGCCGGCTTCGTGCCAGCTGTTTACCGATTTGCTGACAGGGGCCGAGCCGATTATCGACCCGGCGCCGTATG‑CGCCAGAAGGGCGTTTGGGCTGAGGACCTCTTCGCGGGTAAACCCGCTCCTACGCGAGCGGGTTTACCCGCG‑AAAGGGCCGCAGAAGCCAATACAGATTTCAGCCAGTAATCGGTGCTTGCAGGTGCCGGCTCGGATAATGCTGCTCATACACCTTGCACACCCGCAGCACTTGCTCATCAGCAAAGCGCCCGGC > NC_002947/718312‑718602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |