Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	97,592	G→T	10.5%	intergenic (+21/+15)	PP_0090 → / ← PP_0091	hypothetical protein/putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	97,592	0	G	T	10.5%	92.1 / 2.9	44	intergenic (+21/+15)	PP_0090/PP_0091	hypothetical protein/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (21/18);  new base T (3/2);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01

CCAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGT  >  NC_002947/97448‑97737                                                                                                                                                 |                                                                                                                                                  ccAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAagc                                                                                                                                               <  1:19486/149‑1 (MQ=255)   aaGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAagcag                                                                                                                                             >  5:82217/1‑149 (MQ=255)    aGGGGTTTCGTCCTACACGCGGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAagcagc                                                                                                                                            <  7:205895/149‑1 (MQ=255)         tttCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGGTGGGGAGCCGCCCcac                                                                                                                                       >  6:51361/1‑149 (MQ=255)         tttCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCTTCGCAGccccgg                                                                                                                                       <  8:79544/149‑3 (MQ=255)         tttCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCca                                                                                                                                        >  4:260811/1‑148 (MQ=255)                       cTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATg                                                                                                                           >  5:108494/1‑147 (MQ=255)                                  gCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAAAACTTCGc                                                                                                              >  3:84463/1‑149 (MQ=255)                                          gcttgttCAAGGACCAGAACATGGTCGTCGGGACGTGCGAGGGCGGGACTAACAAGATCATATATCACAACACCGATCCATAAGCATCTGGGCCCGGGGCGGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCg                                                                                                      <  4:84463/143‑1 (MQ=255)                                              ggTCAAGGACCATAACATGGTCGTCGGGACCTGCGATGGCGGGACTAAGAAGATCATCTATCATAACAACGATCTATAACCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGtt                                                                                                    <  6:114279/147‑1 (MQ=255)                                                 cAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCCCACTCACGGGATGCAGAACACATCGCAAGGCTCGTTcacca                                                                                               >  2:238194/1‑149 (MQ=255)                                                  aaGGACCAGAACATGGTAGTCGCGACCTGCGATGGCGGGACGAAGAAGATCATCTACCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGCGATGCAGAACACGTCCCTAGGCTCGTTccacac                                                                                              >  7:139609/1‑144 (MQ=255)                                                       ccAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGGGAAGCAGCCACCCACAAACGGGCTGCACAACAAATCGCAATGCGCGTTCacccacacc                                                                                          >  2:124758/1‑148 (MQ=255)                                                            aCATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCg                                                                                     <  6:108494/148‑1 (MQ=255)                                                             cttggtggtggGGACCTGGGATGGCGGGACGAAGAAGATCATCTACCATACCAACCATGGATAAGCTTCTGGGCCCGGGGCTTCGAAGCTGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGt                                                                                   <  8:139609/147‑1 (MQ=255)                                                                ggTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCa                                                                                                                         >  6:100606/1‑108 (MQ=255)                                                                ggTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCa                                                                                                                         <  5:100606/108‑1 (MQ=255)                                                                              gCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACCCTCACGGGATGAAGAACACATCTCTAGGCTCGTTCACCACCAACTTTCGGTTGGCGTAAAATagcag                                                                   >  5:8556/1‑148 (MQ=255)                                                                              gCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACGCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCCCCACCGTGCGGGTGGCGGGATATagcag                                                                   >  4:80049/1‑148 (MQ=255)                                                                                       ggACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTAACCACCACCTTGCGGTTGGCGTGATATAGCAGCACCTGCGGc                                                         >  7:274765/1‑149 (MQ=255)                                                                                          cGAATAAGATCATCTATCAGAATAACGATCTATACGCATCAGGGCCCGGGGCTGCTAACCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTcc                                                      <  8:274765/149‑1 (MQ=255)                                                                                                atatcatcTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCGGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCAGCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCAc                                                 <  5:218362/146‑1 (MQ=255)                                                                                                 gaTCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCCCCACCTTGCGGTTGGCGTCATATTGCAGCACCTGCGGCTCCCTCACCg                                               >  7:73157/1‑149 (MQ=255)                                                                                                      tcTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACGCACGGGCTGCAGAACACATAGCTAGGCTCGTTGACCACCACCGTGCGGGTGGCGTCATATTGCACCACCTGCGGCGCCATCACCGGCGcc                                          >  3:177460/1‑149 (MQ=255)                                                                                                          tCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCAACACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGccc                                         <  1:238194/146‑1 (MQ=255)                                                                                                          tCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACGAACGGGATGCAGAAAACATAGCTAGGGGAGTTAAACACCACCATGCGGGTTGCGTCAAAAAACAGCACCAGCGGCTGCCACAACCGCGcccccc                                      >  4:149559/1‑146 (MQ=255)                                                                                                               agaaCGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTcc                                 <  6:82217/149‑1 (MQ=255)                                                                                                                  aCGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAgg                              <  4:131348/149‑1 (MQ=255)                                                                                                                       cgatAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCcgc                                       >  6:69772/1‑135 (MQ=255)                                                                                                                       cgatAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCcgc                                       <  5:69772/135‑1 (MQ=255)                                                                                                                        gatAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAg                        >  7:225880/1‑149 (MQ=255)                                                                                                                          tacgCATCAGGGCCCGGGGCTGCGACGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAgcg                      <  4:177460/146‑1 (MQ=255)                                                                                                                          tAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAgc                       <  3:149559/148‑1 (MQ=255)                                                                                                                                 cAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCg               >  1:60079/1‑149 (MQ=255)                                                                                                                                  aGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCg               <  8:225880/148‑1 (MQ=255)                                                                                                                                    ggCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGGTGGCGTCATATAGCCGCCCCAGCGGCTCCATCACCGGCGCCCGCGCGTCCAGGCGGTAGCGCTCGCCGGcc            >  2:57721/1‑149 (MQ=255)                                                                                                                                    ggCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGAAGCAGAACACATAGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCCGCGCCCGCGCTTCCAGGCGATAGCGCTAGCCGGc             >  2:65617/1‑148 (MQ=255)                                                                                                                                        tggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTg         >  6:264074/2‑148 (MQ=255)                                                                                                                                         ggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTTGCGTCATATAGCATCACCTGCGGCTCCAACACCCCCGCCCGCGCTTCCAAGCGATAGCGCTCGCAGGCCATg         >  5:165590/1‑147 (MQ=255)                                                                                                                                         ggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTg         >  5:59739/1‑147 (MQ=255)                                                                                                                                         ggggCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGaa       <  3:260811/149‑1 (MQ=255)                                                                                                                                              tGCGACGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGt  <  3:97416/149‑1 (MQ=255)                                                                                                                                              ggCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGt  <  6:309501/148‑1 (MQ=255)                                                                                                                                               gCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGa                           >  2:262297/1‑123 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CCAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGT  >  NC_002947/97448‑97737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑