Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	747,418	C→T	7.5%	intergenic (‑545/‑249)	PP_0638 ← / → PP_5448	transposase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	747,418	0	C	T	7.5%	191.0 / 4.2	66	intergenic (‑545/‑249)	PP_0638/PP_5448	transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (30/31);  new base T (2/3);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

CCAACGGGGGCGTTGCTCGCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCT  >  NC_002947/747270‑747566                                                                                                                                                     |                                                                                                                                                      ccAACGGGGGCGTTGCTCGCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAAcc                                                                                                                                                       >  5:258848/1‑149 (MQ=255)           cGTTGCTCGCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTgg                                                                                                                                             <  1:198106/149‑1 (MQ=255)             ttGCTCGCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGa                                                                                                                                           <  7:49212/149‑1 (MQ=255)                 tCGCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTg                                                                                                                                      >  6:23895/1‑149 (MQ=255)                  cGCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACTCCCAGGATGGGGG‑TTgg                                                                                                                                     <  3:221264/149‑1 (MQ=255)                   gCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACTCCCAGGATGGGGG‑TTGGc                                                                                                                                    <  6:39114/149‑1 (MQ=255)                   gCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTgg                                                                                                                                     <  8:206043/148‑1 (MQ=255)                       cTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGAtt                                                                                                                                >  6:255169/1‑149 (MQ=255)                          aGCTTGATGTTCTCGTTCTATGGGAATCTTCAAAAAAGCACTGAGATTCTACCGGCAGTGATTTCGGTGGTGTAACGACGCGACTGACTGTCACTAAAATCCCATCAGGATACTTTGGCCAACCCCCTTCCTGGGAG‑TTGGAGAttttt                                                                                                                             <  2:285599/149‑1 (MQ=255)                          aGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGAttt                                                                                                                               <  6:78754/147‑1 (MQ=255)                          aGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGAttt                                                                                                                               <  5:23895/147‑1 (MQ=255)                           gCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACTCCCAGGATGGGGG‑TTGGccaca                                                                                                                                <  1:83382/145‑5 (MQ=255)                           gCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACTCCCAGGATGGGGG‑TTGGccaca                                                                                                                                >  2:83382/1‑141 (MQ=255)                           gCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTttg                                                                                                                            <  3:168438/149‑1 (MQ=255)                            cTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTttgt                                                                                                                           >  3:49895/1‑149 (MQ=255)                               gATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCTACCCCCATCCTGGGAG‑TTGGCGATTTttgttg                                                                                                                         <  1:36595/148‑1 (MQ=255)                                     cgcgTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGa                                                                                                                       <  3:79295/144‑1 (MQ=255)                                        tttCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGTAGGTGTAACGACGCCACTGACTGTCACTCTAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGAt                                                                                                               <  1:171274/148‑1 (MQ=255)                                        gTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGAt                                                                                                               <  4:49895/149‑1 (MQ=255)                                         ttCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGAt                                                                                                               >  1:122281/1‑148 (MQ=255)                                                ggAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGacacac                                                                                                        >  8:293408/1‑148 (MQ=255)                                                ggAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGacacac                                                                                                        >  6:104019/1‑148 (MQ=255)                                                 gAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGa                                                                                                                       >  5:254221/1‑132 (MQ=255)                                                   aTCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGaaa                                                                                                    <  4:179479/149‑1 (MQ=255)                                                      ttCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAatca                                                                                                 >  5:112667/1‑149 (MQ=255)                                                      ttCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAatca                                                                                                 >  6:69639/1‑149 (MQ=255)                                                       tCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAatcat                                                                                                >  6:75608/1‑149 (MQ=255)                                                         aaaaaaCCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAatcatc                                                                                               <  7:293408/148‑1 (MQ=255)                                                            aaaCCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGac                                                                                                            <  3:186423/132‑1 (MQ=255)                                                            aaaCCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGac                                                                                                            >  4:186423/1‑132 (MQ=255)                                                                 aCTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGt                                                                                      <  5:255169/149‑1 (MQ=255)                                                                  cTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGCTTAGCGTg                                                                                     >  7:205475/1‑149 (MQ=255)                                                                               ggCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTGAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATg                                                                        <  6:61459/149‑1 (MQ=255)                                                                                  aGTCATTGCGGAGGTGTAACTACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTag                                                                     >  3:167024/1‑149 (MQ=255)                                                                                    tCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTagga                                                                   <  5:104019/149‑1 (MQ=255)                                                                                      aTTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAatcatc                                                                                               <  2:186568/119‑1 (MQ=255)                                                                                      aTTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAatcatc                                                                                               >  1:186568/1‑119 (MQ=255)                                                                                      aTTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTaggag                                                                  >  6:132256/1‑148 (MQ=255)                                                                                              ggTGTAACTACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGt                                                         <  4:167024/149‑1 (MQ=255)                                                                                                 gtAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTAt                                                       >  4:150261/1‑148 (MQ=255)                                                                                                      gacgCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACCCGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGCTCTCCGTATGAGAAc                                                 >  5:238721/1‑149 (MQ=255)                                                                                                       acgCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACg                                                >  2:210438/1‑149 (MQ=255)                                                                                                       acgCCACTGACTGTCACTCAAATCCAATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACg                                                <  8:205475/149‑1 (MQ=255)                                                                                                              tgactgTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTttgt                                         >  5:276382/1‑149 (MQ=255)                                                                                                                actgTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTttgtt                                        <  6:258848/148‑1 (MQ=255)                                                                                                                actgTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTttgtt                                        <  6:112667/148‑1 (MQ=255)                                                                                                                  tgTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCa                                                                                 <  7:87482/105‑1 (MQ=255)                                                                                                                  tgTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCa                                                                                 >  8:87482/1‑105 (MQ=255)                                                                                                                        tCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATga                                                     <  5:131695/127‑1 (MQ=255)                                                                                                                        tCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATga                                                     >  6:131695/1‑127 (MQ=255)                                                                                                                        tCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTttgtt                                        <  1:142480/140‑1 (MQ=255)                                                                                                                        tCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTttgtt                                        >  2:142480/1‑140 (MQ=255)                                                                                                                        tCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTgg                               >  7:243721/1‑149 (MQ=255)                                                                                                                          aaaTCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGATATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGc                             >  5:228661/1‑149 (MQ=255)                                                                                                                                 aTCAGCATACTGTGGCCAACCCCCATCCTGGGAGTTTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGc                         >  1:49959/1‑147 (MQ=255)                                                                                                                                 aTCAGCATACTGTGGCCAAACCCCATCCTGGCAG‑TTGGCGATATTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAg                      >  3:296516/1‑149 (MQ=255)                                                                                                                                     gCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGATATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCa                  <  6:228661/149‑1 (MQ=255)                                                                                                                                       aTACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAgg                >  6:60133/1‑149 (MQ=255)                                                                                                                                          cTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGc                   <  3:51973/143‑1 (MQ=255)                                                                                                                                          cTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGc                   >  4:51973/1‑143 (MQ=255)                                                                                                                                           tgtgGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCTTGTAGGAGGATCTCCGGATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTc            >  8:273921/1‑149 (MQ=255)                                                                                                                                            gtgGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGt                                                         >  4:261973/1‑103 (MQ=255)                                                                                                                                            gtgGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGt                                                         <  3:261973/103‑1 (MQ=255)                                                                                                                                             tgGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCtg          <  5:75608/149‑1 (MQ=255)                                                                                                                                             tgGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCt           <  8:243721/148‑1 (MQ=255)                                                                                                                                               gCCAACTCCCAGGATGGGGG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCtgtg        >  2:273448/1‑149 (MQ=255)                                                                                                                                                ccAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCtgtgt       <  5:132256/149‑1 (MQ=255)                                                                                                                                                     ccccATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGct  >  3:273086/1‑149 (MQ=255)                                                                                                                                                     ccccATCCTGGGAC‑TTGGCGATTTTTGTTGGAGGGACGATCACACACCAAATCATCCGTTAGCGTGTAAACTCACCATGTAGGAGGATCTCCGTATCAGAACGGTTATGATCTGTCTTGGGCTTGCAAGTGCAGGCTTATCTCTGGGc   >  4:248873/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CCAACGGGGGCGTTGCTCGCACCTGAGCTTGATCTACGCGTTCTCTGGGAATCTTCAAAAAACCACTGAGATTCTACCGGCAGTCATTGCGGAGGTGTAACGACGCCACTGACTGTCACTCAAATCCCATCAGCATACTGTGGCCAACCCCCATCCTGGGAG‑TTGGCGATTTTTGTTGGAGGGACGATGACACACGAAATCATCGGTTAGCGTGGCCACTCACCATGTAGGAGGATCTCCGTATGAGAACGGTTTTGTTGTGTCTTGGGCTTGCAAGTGCAGGCTTCTGTGTGGGCT  >  NC_002947/747270‑747566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑