Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 753,230 T→C 7.4% intergenic (‑469/‑514) PP_0641 ← / → PP_5452 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947753,2300TC 7.4% 157.0 / 2.5 54intergenic (‑469/‑514)PP_0641/PP_5452hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (26/24);  new base C (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

GAGGCATCAGTGCGCCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGCAAACTGCCCAAATATCCGCTTTTCCGGG  >  NC_002947/753088‑753377
                                                                                                                                              |                                                                                                                                                   
gAGGCATCAGTGCGCCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTAt                                                                                                                                                  >  4:42882/1‑146 (MQ=255)
gAGGCATCAGTGCGCCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTAt                                                                                                                                                  <  3:42882/146‑1 (MQ=255)
 aGGCATCAGTGCGCCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCat                                                                                                                                              >  6:211181/1‑149 (MQ=255)
  ggCATCAGTGCGCCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACtagatcgt                                                                                                                                              >  3:144686/1‑141 (MQ=255)
     aTCAGTGCGCCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCattat                                                                                                                                           >  1:82821/1‑148 (MQ=255)
         gTGCGCCTACTACCCAACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTAAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAg                                                                                                                                      >  7:20577/1‑149 (MQ=255)
          tGCGCCTACTCCCCCACCACCATGTCCTCACTTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAgg                                                                                                                                     <  5:76326/149‑1 (MQ=255)
            cgcCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGGCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAggg                                                                                                                                    <  2:2059/148‑1 (MQ=255)
            cgcCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAggg                                                                                                                                    <  5:211181/148‑1 (MQ=255)
             gcCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGgt                                                                                                                                  >  6:134817/1‑149 (MQ=255)
               cTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGgtg                                                                                                                                 >  8:113482/1‑148 (MQ=255)
                           caccaTGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAAtt                                                                                                                    <  8:252027/149‑1 (MQ=255)
                              caTGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGa                                                                                                                 <  7:113482/149‑1 (MQ=255)
                                tGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCAAGTGATCGTCAGGCTGGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGAt                                                                                                                >  6:306466/1‑148 (MQ=255)
                                    cTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGAt                                                                                                                >  3:35913/1‑144 (MQ=255)
                                    cTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGAt                                                                                                                <  4:35913/144‑1 (MQ=255)
                                    cTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTg                                                                                                           >  8:173946/1‑149 (MQ=255)
                                    cTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCATTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCt                                                                                                            <  1:97088/148‑1 (MQ=255)
                                      cACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCattatt                                                                                                                                          <  7:242893/116‑1 (MQ=255)
                                      cACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCattatt                                                                                                                                          >  8:242893/1‑116 (MQ=255)
                                       aCGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGtct                                                                                                        <  2:82821/149‑1 (MQ=255)
                                         gTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTcc                                                                                                      >  6:166507/1‑149 (MQ=255)
                                           cccGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACCTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCg                                                                                                     >  1:11962/1‑148 (MQ=255)
                                           cccGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACCTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCg                                                                                                     >  1:7216/1‑148 (MQ=255)
                                               gTTGGATTGCGGTGACTGTTTCCGTCCAGTGTTAGTCAGGCTCGTTCAGGTTTAGTGCCTTTTTGAGTAGCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGTGTGTACGGCACAAATTCGATTCCTGTCTGCGCACCa                                                                                                <  8:20577/149‑1 (MQ=255)
                                                    aTTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAg                                                                                               <  2:68680/145‑1 (MQ=255)
                                                    aTTCCGGTAACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAg                                                                                               >  1:68680/1‑145 (MQ=255)
                                                      tCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGtcaat                                                                                          >  4:154566/1‑148 (MQ=255)
                                                                gTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGa                                                                                                                 <  4:210906/115‑1 (MQ=255)
                                                                gTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGa                                                                                                                 >  3:210906/1‑115 (MQ=255)
                                                                 tttCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCa                                                                              >  8:177369/1‑149 (MQ=255)
                                                                     cGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGTGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAgcgc                                                                          <  2:218760/149‑1 (MQ=255)
                                                                      gCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAgcgc                                                                          >  4:96956/1‑148 (MQ=255)
                                                                          aGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTccc                                                                     >  4:51568/1‑149 (MQ=255)
                                                                              aTCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCt                                                                  <  7:177369/148‑1 (MQ=255)
                                                                                 gTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCt                                                                                                            <  7:79537/103‑1 (MQ=255)
                                                                                 gTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCt                                                                                                            >  8:79537/1‑103 (MQ=255)
                                                                                   cAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCt                                                                                                            <  6:149978/101‑1 (MQ=255)
                                                                                   cAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCt                                                                                                            >  5:149978/1‑101 (MQ=255)
                                                                                     ggCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTc                                                          <  7:173946/149‑1 (MQ=255)
                                                                                               ggTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCACGCTATCACCTCCCGCCCACCg                                                >  3:15073/1‑149 (MQ=255)
                                                                                                    agcgCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGc                                             >  4:268419/4‑147 (MQ=255)
                                                                                                        ccTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCcagcag                                       <  3:96956/149‑1 (MQ=255)
                                                                                                        ccTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCcagca                                        <  5:306466/148‑1 (MQ=255)
                                                                                                         cTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCagcagc                                      <  5:134817/149‑1 (MQ=255)
                                                                                                         cTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCagcagc                                      <  3:51568/149‑1 (MQ=255)
                                                                                                            tttGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAATGGGTGTACGGAACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATAACCTCCCGCCCAGCGTGCCAGCAGCGt                                    <  6:267770/148‑1 (MQ=255)
                                                                                                              tGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGa                                  <  5:166507/148‑1 (MQ=255)
                                                                                                                     cTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGCAAAc                          >  4:223019/1‑149 (MQ=255)
                                                                                                                     cTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGCAAAc                          >  2:175778/1‑149 (MQ=255)
                                                                                                                     cTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGAAAAc                          >  2:171234/1‑149 (MQ=255)
                                                                                                                             tttATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGCAAACTGCCCaaa                  >  1:221438/1‑149 (MQ=255)
                                                                                                                                aTGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGCAAACTGCCCAAatat               >  3:72095/1‑149 (MQ=255)
                                                                                                                                   aaaTACGATACCTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGGGTGCCAGCAGCGTGACAGCAAAGTGCCCAAATATCCg            <  2:7216/149‑1 (MQ=255)
                                                                                                                                    aaTACGATACCTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGCAAACTGCCCAAATATCCg            <  2:11962/148‑1 (MQ=255)
                                                                                                                                              ttATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCca                                           <  3:151690/107‑1 (MQ=255)
                                                                                                                                              ttATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCca                                           >  4:151690/1‑107 (MQ=255)
                                                                                                                                              ttATCCATTATTAAAGGGGTGTAAGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTAGCACCTCCCGCCCAGCGTGCAAGCAGCGGGAGAGCAAACTGAGCAAATATACGCTTTTCCgtg  >  6:227134/1‑146 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
GAGGCATCAGTGCGCCTACTCCCCCACCACCATGTCCTCACGTCCCGGTTCGATTCCGGTCACTGTTTCCGCCCAGTGATCGTCAGGCTCGATCAGGTTGAGTGCCTTTTTGAGTACCTATTCACTTTATGAAATACGATACTTATCCATTATTAAAGGGGTGTACGGCACAAATTCGATTCCTGTCTCCGCACCAGTCAATTCAATGCCTCCAGCGCCTCCCGCTATCACCTCCCGCCCAGCGTGCCAGCAGCGTGACAGCAAACTGCCCAAATATCCGCTTTTCCGGG  >  NC_002947/753088‑753377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑