Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 754,762 T→G 8.9% intergenic (‑18/+150) PP_0643 ← / ← PP_0645 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947754,7620TG 8.9% 128.7 / 2.6 43intergenic (‑18/+150)PP_0643/PP_0645hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/15);  new base G (2/2);  total (26/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTGCAGCGGCAGCAGCCGGCGCGCGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGCTCTGGCGCGC  >  NC_002947/754614‑754909
                                                                                                                                                    |                                                                                                                                                   
aCGTGCAGCGGCAGCATCCGGCGCGCGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAatt                                                                                                                                                     <  3:189405/149‑1 (MQ=255)
          gcagcaGCCGGCGCGCGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCgg                                                                                                                                           >  5:239400/1‑149 (MQ=255)
          gcagcaGCCGGCGCGCGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCgg                                                                                                                                           >  4:54440/1‑149 (MQ=255)
               agcCGGCGCGCGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCgtt                                                                                                                                      >  7:289507/1‑149 (MQ=255)
                 ccGGCGCGCGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGc                                                                                                                                    <  3:54440/149‑1 (MQ=255)
                    gcgcgcGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCGCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTg                                                                                                                                 <  8:52691/149‑1 (MQ=255)
                    gcgcgcGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTg                                                                                                                                 <  5:237643/149‑1 (MQ=255)
                      gcgcgGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGtt                                                                                                                               <  5:164459/149‑1 (MQ=255)
                                    gtcACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATcc                                                                                                                 >  4:51906/1‑149 (MQ=255)
                                     tcACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCcg                                                                                                                >  4:196063/1‑149 (MQ=255)
                                         ctggctggATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGATATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCga                                                                                                                       <  6:306361/138‑1 (MQ=255)
                                         ctggctggATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGATATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCga                                                                                                                       >  5:306361/1‑138 (MQ=255)
                                            gctggATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATg                                                                                                          <  3:43400/148‑1 (MQ=255)
                                              tggATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGtt                                                                                                       >  5:69801/1‑149 (MQ=255)
                                                            gCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGc                                                                                         <  2:297278/149‑1 (MQ=255)
                                                              cAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGcc                                                                                        >  8:8964/1‑148 (MQ=255)
                                                                        ggCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAg                                                                              <  3:196063/148‑1 (MQ=255)
                                                                               ccGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGc                                                                             >  6:3385/1‑142 (MQ=255)
                                                                               ccGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGc                                                                             <  5:3385/142‑1 (MQ=255)
                                                                                cgcgTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCt                                                                      >  3:25480/1‑148 (MQ=255)
                                                                                cgcgTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTg                                                                     <  6:239400/149‑1 (MQ=255)
                                                                                cgcgTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCAAGGCCCAACATCGAGCCGAAGCt                                                                      >  2:112353/1‑148 (MQ=255)
                                                                                 gcgTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGa                                                                    >  1:137055/1‑149 (MQ=255)
                                                                                            gTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGc                                                                             >  4:81314/1‑129 (MQ=255)
                                                                                            gTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGc                                                                             <  3:81314/129‑1 (MQ=255)
                                                                                              gCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCa                                                        >  3:114015/1‑148 (MQ=255)
                                                                                                       gcgcgcGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGc                                                                       <  1:191534/124‑1 (MQ=255)
                                                                                                       gcgcgcGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGc                                                                       >  2:191534/1‑124 (MQ=255)
                                                                                                        cgcgcgGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATtg                                             >  2:255982/1‑149 (MQ=255)
                                                                                                         gcgcgGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATtgg                                            >  3:207742/1‑149 (MQ=255)
                                                                                                             ggACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTggc                                        >  4:235720/1‑149 (MQ=255)
                                                                                                                   tCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGGTcc                                                                                             >  8:226976/1‑90 (MQ=255)
                                                                                                                     gcgAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCggccagg                                 <  1:38357/148‑1 (MQ=255)
                                                                                                                           tCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGggccaggc                          >  7:21548/1‑149 (MQ=255)
                                                                                                                                 aTTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCAcccc                    >  6:8723/1‑149 (MQ=255)
                                                                                                                                 aTTCACCAGGGCGGCGAATGCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGGTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCAcccc                    <  5:192681/149‑1 (MQ=255)
                                                                                                                                     caaagcccggcgAATGAATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCGGGGCCAGGCCACCCCtgtg                <  4:5119/142‑1 (MQ=255)
                                                                                                                                      gcagGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCtgtg                >  2:235770/1‑148 (MQ=255)
                                                                                                                                      gcagGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGa               <  1:235770/149‑1 (MQ=255)
                                                                                                                                          ggcggcGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGt                                                                                                        >  7:14869/1‑56 (MQ=255)
                                                                                                                                          ggcggcGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGt                                                                                                        <  8:14869/56‑1 (MQ=255)
                                                                                                                                           ccggcgAATGAATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGctc          >  2:109026/2‑149 (MQ=255)
                                                                                                                                            cggcgAATGAATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGctct         >  1:261136/1‑149 (MQ=255)
                                                                                                                                                 aaTTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGCTCTGgcgc    >  3:161254/1‑149 (MQ=255)
                                                                                                                                                   ttcattcGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGCTCTg        >  6:192410/1‑143 (MQ=255)
                                                                                                                                                   ttcattcGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGCTCTg        <  5:192410/143‑1 (MQ=255)
                                                                                                                                                   ttcattcGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGCTCTGgcgcgc  <  7:8964/149‑1 (MQ=255)
                                                                                                                                                   ttcattcGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGCTCTGgcgcgc  <  5:8723/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACGTGCAGCGGCAGCAGCCGGCGCGCGGTTTCGTCCGTCACCTGGCTGGATATCTTGGTGGCCAGCGACTTGGGCACGCCCGCGTTGCGCAGGTGCCGCTCCAGCGCGCGGACGGTCGCGAAATCACGCATTCAGCAGGGCGGCGAATTCATTCGCCGGGCTTTGCCTGTTGCCCCCGATGATCCGCGCATGGTTGGCCGGCTCCAGGCCCAACATCGAGCCGAAGCTGATCATCTGGCGCATAGCCTCATTGGTGGCGGCCAGGGCCAGGCCACCCCTGTGAAGCTCTGGCGCGC  >  NC_002947/754614‑754909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑