Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 766,609 A→T 9.8% Q34L (CAG→CTG)  metB → cystathionine gamma‑synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947766,6090AT 9.8% 135.5 / 4.5 51Q34L (CAG→CTG) metBcystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base A (25/21);  new base T (2/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

CGATGCTGCATCGGTGTACACAACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCTGG  >  NC_002947/766464‑766755
                                                                                                                                                 |                                                                                                                                                  
cGATGCTGCATCGGTGTACACAACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGAc                                                                                                                                                 <  1:295596/149‑1 (MQ=255)
              tgtACACAACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCa                                                                                                                                   <  1:129552/149‑1 (MQ=255)
                  cacaACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCTGACCACGATCGGGGTCTgcg                                                                                                                                <  1:158914/148‑1 (MQ=255)
                  cacaACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAgcgc                                                                                                                               >  8:256391/1‑149 (MQ=255)
                    caACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCTGACCACGATCGGGGTCTgcgtgg                                                                                                                             <  1:195990/149‑4 (MQ=255)
                     aaCTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGcgccgc                                                                                                                            >  8:176510/1‑149 (MQ=255)
                     aaCTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGcgccgc                                                                                                                            >  2:66077/1‑149 (MQ=255)
                           gCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATgg                                                                                                                      <  4:196576/149‑1 (MQ=255)
                            cTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGc                                                                                                                     >  7:225517/1‑149 (MQ=255)
                             tGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGc                                                                                                                     >  6:264677/1‑148 (MQ=255)
                                gCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTAc                                                                                                                  >  2:73706/1‑148 (MQ=255)
                                 cGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCa                                                                                                                                   <  4:6562/130‑1 (MQ=255)
                                 cGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCa                                                                                                                                   >  3:6562/1‑130 (MQ=255)
                                     aaCCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGa                                                                                                             >  5:298417/1‑148 (MQ=255)
                                       ccGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACAt                                                                                                          <  1:73706/149‑1 (MQ=255)
                                        cGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATc                                                                                                         <  7:176510/149‑1 (MQ=255)
                                           atatGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGAt                                                                                                      <  7:84414/149‑1 (MQ=255)
                                           atatGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGAt                                                                                                      <  6:298417/149‑1 (MQ=255)
                                                 acaacaacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTg                                                                                                 <  7:256391/148‑1 (MQ=255)
                                                   aacaacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTa                                                                                              <  2:282932/149‑1 (MQ=255)
                                                     caacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTAt                                                                                                                        >  7:118089/1‑121 (MQ=255)
                                                     caacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTAt                                                                                                                        <  8:118089/121‑1 (MQ=255)
                                                      aacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTacga                                                                                           >  6:307160/1‑149 (MQ=255)
                                                          aaaaaTTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGc                                                                                                    <  3:130321/136‑1 (MQ=255)
                                                          aaaaaTTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGAGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGc                                                                                                    >  4:130321/1‑136 (MQ=255)
                                                           aaaaTTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTgg                                                                                      <  8:225517/149‑1 (MQ=255)
                                                                  gAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTg                                                                                                 <  6:173337/131‑1 (MQ=255)
                                                                  gAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTg                                                                                                 >  5:173337/1‑131 (MQ=255)
                                                                   aGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGCTGCCTGGTACGACGTGGCGCAggg                                                                               >  8:242074/1‑148 (MQ=255)
                                                                   aGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAggg                                                                               >  4:66509/1‑148 (MQ=255)
                                                                           gctgcGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGcc                                                                      >  7:230224/1‑149 (MQ=255)
                                                                                  ccGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTc                                                               <  5:307160/149‑1 (MQ=255)
                                                                                      tgtgGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCGTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATc                                                            >  8:256822/1‑148 (MQ=255)
                                                                                          gggACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACa                                                        <  1:66077/148‑1 (MQ=255)
                                                                                                cgGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCAt                                                 <  5:264677/149‑1 (MQ=255)
                                                                                                    tCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAg                                              >  3:301160/1‑148 (MQ=255)
                                                                                                                gggAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTc                                 >  6:115723/1‑149 (MQ=255)
                                                                                                                     cAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGc                                             >  7:206339/1‑132 (MQ=255)
                                                                                                                     cAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGc                                             <  8:206339/132‑1 (MQ=255)
                                                                                                                        gTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCTCACGGTCGCCACGCt                         >  8:135788/1‑149 (MQ=255)
                                                                                                                             gCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAg                    <  2:211807/149‑1 (MQ=255)
                                                                                                                              cACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAg                    >  7:225576/1‑148 (MQ=255)
                                                                                                                               aCCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGc                   >  5:259973/1‑148 (MQ=255)
                                                                                                                                 ccGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCaa                 >  3:138020/1‑148 (MQ=255)
                                                                                                                                         cGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCg        <  6:259973/149‑1 (MQ=255)
                                                                                                                                          gCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGc          >  2:170583/1‑146 (MQ=255)
                                                                                                                                          gCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGc          <  1:170583/146‑1 (MQ=255)
                                                                                                                                             gcGCTGACCACGATCGGGGTCTGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCt    >  1:156181/2‑149 (MQ=255)
                                                                                                                                             gcGCTGACCACGATCGGGGTCTGCGCCGCTTATGGCTACAACGACATCGATGCATGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTgg                       <  1:86464/129‑1 (MQ=255)
                                                                                                                                             gcGCTGACCACGATCGGGGTCTGCGCCGCTTATGGCTACAACGACATCGATGACTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCAACAGCAAACACACGGTCGCCACCCTga                       >  2:86464/2‑129 (MQ=255)
                                                                                                                                               gCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCTgg  >  5:101669/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGATGCTGCATCGGTGTACACAACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCTGG  >  NC_002947/766464‑766755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑