Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 766,614 C→A 9.8% P36T (CCG→ACG)  metB → cystathionine gamma‑synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947766,6140CA 9.8% 145.9 / 4.9 50P36T (CCG→ACG) metBcystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base C (25/20);  new base A (2/3);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

TGTACACAACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCTGGAGCAAG  >  NC_002947/766478‑766761
                                                                                                                                        |                                                                                                                                                   
tgtACACAACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCa                                                                                                                                         <  1:129552/149‑1 (MQ=255)
    cacaACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCTGACCACGATCGGGGTCTgcg                                                                                                                                      <  1:158914/148‑1 (MQ=255)
    cacaACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAgcgc                                                                                                                                     >  8:256391/1‑149 (MQ=255)
      caACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCTGACCACGATCGGGGTCTgcgtgg                                                                                                                                   <  1:195990/149‑4 (MQ=255)
       aaCTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGcgccgc                                                                                                                                  >  2:66077/1‑149 (MQ=255)
       aaCTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGcgccgc                                                                                                                                  >  8:176510/1‑149 (MQ=255)
             gCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATgg                                                                                                                            <  4:196576/149‑1 (MQ=255)
              cTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGc                                                                                                                           >  7:225517/1‑149 (MQ=255)
               tGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGc                                                                                                                           >  6:264677/1‑148 (MQ=255)
                  gCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTAc                                                                                                                        >  2:73706/1‑148 (MQ=255)
                   cGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCa                                                                                                                                         >  3:6562/1‑130 (MQ=255)
                   cGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCa                                                                                                                                         <  4:6562/130‑1 (MQ=255)
                       aaCCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGa                                                                                                                   >  5:298417/1‑148 (MQ=255)
                         ccGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACAt                                                                                                                <  1:73706/149‑1 (MQ=255)
                          cGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATc                                                                                                               <  7:176510/149‑1 (MQ=255)
                             atatGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGAt                                                                                                            <  7:84414/149‑1 (MQ=255)
                             atatGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGAt                                                                                                            <  6:298417/149‑1 (MQ=255)
                                   acaacaacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTg                                                                                                       <  7:256391/148‑1 (MQ=255)
                                     aacaacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTa                                                                                                    <  2:282932/149‑1 (MQ=255)
                                       caacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTAt                                                                                                                              >  7:118089/1‑121 (MQ=255)
                                       caacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTAt                                                                                                                              <  8:118089/121‑1 (MQ=255)
                                        aacGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTacga                                                                                                 >  6:307160/1‑149 (MQ=255)
                                            aaaaaTTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGc                                                                                                          <  3:130321/136‑1 (MQ=255)
                                            aaaaaTTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGAGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGc                                                                                                          >  4:130321/1‑136 (MQ=255)
                                             aaaaTTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTgg                                                                                            <  8:225517/149‑1 (MQ=255)
                                                    gAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTg                                                                                                       >  5:173337/1‑131 (MQ=255)
                                                    gAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTg                                                                                                       <  6:173337/131‑1 (MQ=255)
                                                     aGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGCTGCCTGGTACGACGTGGCGCAggg                                                                                     >  8:242074/1‑148 (MQ=255)
                                                     aGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAggg                                                                                     >  4:66509/1‑148 (MQ=255)
                                                             gctgcGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGcc                                                                            >  7:230224/1‑149 (MQ=255)
                                                                    ccGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTc                                                                     <  5:307160/149‑1 (MQ=255)
                                                                        tgtgGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCGTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATc                                                                  >  8:256822/1‑148 (MQ=255)
                                                                            gggACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACa                                                              <  1:66077/148‑1 (MQ=255)
                                                                                  cgGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCAt                                                       <  5:264677/149‑1 (MQ=255)
                                                                                      tCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAg                                                    >  3:301160/1‑148 (MQ=255)
                                                                                                  gggAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTc                                       >  6:115723/1‑149 (MQ=255)
                                                                                                       cAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGc                                                   >  7:206339/1‑132 (MQ=255)
                                                                                                       cAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGc                                                   <  8:206339/132‑1 (MQ=255)
                                                                                                          gTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCTCACGGTCGCCACGCt                               >  8:135788/1‑149 (MQ=255)
                                                                                                               gCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAg                          <  2:211807/149‑1 (MQ=255)
                                                                                                                cACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAg                          >  7:225576/1‑148 (MQ=255)
                                                                                                                 aCCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGc                         >  5:259973/1‑148 (MQ=255)
                                                                                                                   ccGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCaa                       >  3:138020/1‑148 (MQ=255)
                                                                                                                           cGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCg              <  6:259973/149‑1 (MQ=255)
                                                                                                                            gCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGc                >  2:170583/1‑146 (MQ=255)
                                                                                                                            gCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGc                <  1:170583/146‑1 (MQ=255)
                                                                                                                               gcGCTGACCACGATCGGGGTCTGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCt          >  1:156181/2‑149 (MQ=255)
                                                                                                                               gcGCTGACCACGATCGGGGTCTGCGCCGCTTATGGCTACAACGACATCGATGCATGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTgg                             <  1:86464/129‑1 (MQ=255)
                                                                                                                               gcGCTGACCACGATCGGGGTCTGCGCCGCTTATGGCTACAACGACATCGATGACTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCAACAGCAAACACACGGTCGCCACCCTga                             >  2:86464/2‑129 (MQ=255)
                                                                                                                                 gCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCTgg        >  5:101669/1‑149 (MQ=255)
                                                                                                                                       cccGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCTGGAGCAAg  >  7:278775/1‑149 (MQ=255)
                                                                                                                                        |                                                                                                                                                   
TGTACACAACTCAGCTGGGCGGGAACCGGATATGAACAACAACGAAAAATTCGAGGGCAATGCTGCGGCCGGTGTGGGGACGCGGGTCGTGTGGAGCGGGGAGCAGGTACAGCACCCGTACAACGCCACGCAGACCCCGATCGTGGTCAGCGCCGCTTATGGCTACAACGACATCGATGCCTGGTACGACGTGGCGCAGGGCAAGCAGCCGGGCTTCATCTACAGCCGCATGAGCAACCCCACGGTCGCCACGCTGGAAGCAAAACTGGCCGAGCTGGAGCAAG  >  NC_002947/766478‑766761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑