Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	97,594	A→C	13.9%	intergenic (+23/+13)	PP_0090 → / ← PP_0091	hypothetical protein/putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	97,594	0	A	C	13.9%	86.1 / 4.5	41	intergenic (+23/+13)	PP_0090/PP_0091	hypothetical protein/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (20/15);  new base C (3/3);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGTCAAA  >  NC_002947/97448‑97741                                                                                                                                                   |                                                                                                                                                    ccAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAagc                                                                                                                                                   <  1:19486/149‑1 (MQ=255)   aaGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAagcag                                                                                                                                                 >  5:82217/1‑149 (MQ=255)    aGGGGTTTCGTCCTACACGCGGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAagcagc                                                                                                                                                <  7:205895/149‑1 (MQ=255)         tttCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGGTGGGGAGCCGCCCcac                                                                                                                                           >  6:51361/1‑149 (MQ=255)         tttCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCTTCGCAGccccgg                                                                                                                                           <  8:79544/149‑3 (MQ=255)         tttCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCca                                                                                                                                            >  4:260811/1‑148 (MQ=255)                       cTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATg                                                                                                                               >  5:108494/1‑147 (MQ=255)                                  gCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAAAACTTCGc                                                                                                                  >  3:84463/1‑149 (MQ=255)                                          gcttgttCAAGGACCAGAACATGGTCGTCGGGACGTGCGAGGGCGGGACTAACAAGATCATATATCACAACACCGATCCATAAGCATCTGGGCCCGGGGCGGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCg                                                                                                          <  4:84463/143‑1 (MQ=255)                                              ggTCAAGGACCATAACATGGTCGTCGGGACCTGCGATGGCGGGACTAAGAAGATCATCTATCATAACAACGATCTATAACCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGtt                                                                                                        <  6:114279/147‑1 (MQ=255)                                                 cAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCCCACTCACGGGATGCAGAACACATCGCAAGGCTCGTTcacca                                                                                                   >  2:238194/1‑149 (MQ=255)                                                  aaGGACCAGAACATGGTAGTCGCGACCTGCGATGGCGGGACGAAGAAGATCATCTACCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGCGATGCAGAACACGTCCCTAGGCTCGTTccacac                                                                                                  >  7:139609/1‑144 (MQ=255)                                                       ccAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGGGAAGCAGCCACCCACAAACGGGCTGCACAACAAATCGCAATGCGCGTTCacccacacc                                                                                              >  2:124758/1‑148 (MQ=255)                                                            aCATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCg                                                                                         <  6:108494/148‑1 (MQ=255)                                                             cttggtggtggGGACCTGGGATGGCGGGACGAAGAAGATCATCTACCATACCAACCATGGATAAGCTTCTGGGCCCGGGGCTTCGAAGCTGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGt                                                                                       <  8:139609/147‑1 (MQ=255)                                                                ggTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCa                                                                                                                             >  6:100606/1‑108 (MQ=255)                                                                ggTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCa                                                                                                                             <  5:100606/108‑1 (MQ=255)                                                                              gCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACCCTCACGGGATGAAGAACACATCTCTAGGCTCGTTCACCACCAACTTTCGGTTGGCGTAAAATagcag                                                                       >  5:8556/1‑148 (MQ=255)                                                                              gCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACGCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCCCCACCGTGCGGGTGGCGGGATATagcag                                                                       >  4:80049/1‑148 (MQ=255)                                                                                       ggACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTAACCACCACCTTGCGGTTGGCGTGATATAGCAGCACCTGCGGc                                                             >  7:274765/1‑149 (MQ=255)                                                                                          cGAATAAGATCATCTATCAGAATAACGATCTATACGCATCAGGGCCCGGGGCTGCTAACCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTcc                                                          <  8:274765/149‑1 (MQ=255)                                                                                                atatcatcTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCGGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCAGCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCAc                                                     <  5:218362/146‑1 (MQ=255)                                                                                                 gaTCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCCCCACCTTGCGGTTGGCGTCATATTGCAGCACCTGCGGCTCCCTCACCg                                                   >  7:73157/1‑149 (MQ=255)                                                                                                      tcTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACGCACGGGCTGCAGAACACATAGCTAGGCTCGTTGACCACCACCGTGCGGGTGGCGTCATATTGCACCACCTGCGGCGCCATCACCGGCGcc                                              >  3:177460/1‑149 (MQ=255)                                                                                                          tCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCAACACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGccc                                             <  1:238194/146‑1 (MQ=255)                                                                                                          tCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACGAACGGGATGCAGAAAACATAGCTAGGGGAGTTAAACACCACCATGCGGGTTGCGTCAAAAAACAGCACCAGCGGCTGCCACAACCGCGcccccc                                          >  4:149559/1‑146 (MQ=255)                                                                                                               agaaCGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTcc                                     <  6:82217/149‑1 (MQ=255)                                                                                                                  aCGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAgg                                  <  4:131348/149‑1 (MQ=255)                                                                                                                       cgatAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCcgc                                           >  6:69772/1‑135 (MQ=255)                                                                                                                       cgatAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCcgc                                           <  5:69772/135‑1 (MQ=255)                                                                                                                        gatAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAg                            >  7:225880/1‑149 (MQ=255)                                                                                                                          tacgCATCAGGGCCCGGGGCTGCGACGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAgcg                          <  4:177460/146‑1 (MQ=255)                                                                                                                          tAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAgc                           <  3:149559/148‑1 (MQ=255)                                                                                                                                 cAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCg                   >  1:60079/1‑149 (MQ=255)                                                                                                                                  aGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCg                   <  8:225880/148‑1 (MQ=255)                                                                                                                                    ggCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGGTGGCGTCATATAGCCGCCCCAGCGGCTCCATCACCGGCGCCCGCGCGTCCAGGCGGTAGCGCTCGCCGGcc                >  2:57721/1‑149 (MQ=255)                                                                                                                                    ggCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGAAGCAGAACACATAGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCCGCGCCCGCGCTTCCAGGCGATAGCGCTAGCCGGc                 >  2:65617/1‑148 (MQ=255)                                                                                                                                        tggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTg             >  6:264074/2‑148 (MQ=255)                                                                                                                                         ggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTTGCGTCATATAGCATCACCTGCGGCTCCAACACCCCCGCCCGCGCTTCCAAGCGATAGCGCTCGCAGGCCATg             >  5:165590/1‑147 (MQ=255)                                                                                                                                         ggggCTGCTTCGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTg             >  5:59739/1‑147 (MQ=255)                                                                                                                                         ggggCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGaa           <  3:260811/149‑1 (MQ=255)                                                                                                                                              tGCGACGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGt      <  3:97416/149‑1 (MQ=255)                                                                                                                                              ggCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGt      <  6:309501/148‑1 (MQ=255)                                                                                                                                               gCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGa                               >  2:262297/1‑123 (MQ=255)                                                                                                                                                  aaGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGTCaaa  >  5:71570/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CCAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCCGGGGCTGCGAAGCAGCCCCACACTCACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGTCAAA  >  NC_002947/97448‑97741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑