Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 797,020 C→A 8.3% A11A (GCC→GCA) ‡ PP_0682 → inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947797,0200CA 8.3% 140.1 / 4.2 48A11A (GCC→GCA) ‡PP_0682inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (24/20);  new base A (2/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

TGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATCCTGGTGATCCTGTCACT  >  NC_002947/796886‑797166
                                                                                                                                      |                                                                                                                                                  
tgtCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGgctgc                                                                                                                                       <  3:84007/148‑1 (MQ=255)
      gATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTGTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGAGGCATACATcgcgttttc                                                                                                                                          >  4:258483/1‑134 (MQ=255)
      gATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTCTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGAGGCATACATcgcgttttc                                                                                                                                          <  3:258483/139‑6 (MQ=255)
        tCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGa                                                                                                                              >  2:62067/1‑149 (MQ=255)
        tCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTAGCACCGa                                                                                                                              >  3:69576/1‑149 (MQ=255)
         cAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGAt                                                                                                                             >  7:203120/1‑149 (MQ=255)
              tGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACt                                                                                                                        >  6:162013/1‑149 (MQ=255)
                cGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTc                                                                                                                      <  4:69576/149‑1 (MQ=255)
                            cGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTc                                                                                                          >  7:291004/1‑149 (MQ=255)
                               gCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGc                                                                                                       <  1:68875/149‑1 (MQ=255)
                                           aCAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTg                                                                                           <  5:30744/149‑1 (MQ=255)
                                                           aCTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTc                                                                            >  2:253631/1‑148 (MQ=255)
                                                             tCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAg                                                                         >  1:198383/1‑149 (MQ=255)
                                                             tCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAg                                                                         >  8:232396/1‑149 (MQ=255)
                                                                ccGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGaa                                                                      <  8:203120/149‑1 (MQ=255)
                                                                   tCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTg                                                                   <  8:291004/149‑1 (MQ=255)
                                                                   tCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGAGATGTATGCCTCGCGATGGCTGATCGCACCGATCTACTTCGGCTTGTCCCTCGGCATGCTGGCCCTGGCGATGAAATTTTTCCAGGAAGTg                                                                   >  7:147117/1‑149 (MQ=255)
                                                                     gCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCTCTGGCGCTGAAATTTTTCCAGGAAGTGGt                                                                 >  5:297753/1‑149 (MQ=255)
                                                                     gCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGt                                                                 >  2:145316/1‑149 (MQ=255)
                                                                      cTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGACGTggac                                                                <  7:232396/149‑3 (MQ=255)
                                                                          tttAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACg                                                            >  3:26297/1‑149 (MQ=255)
                                                                             aGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTcc                                                         >  3:267855/1‑149 (MQ=255)
                                                                              gTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTcc                                                         <  4:26297/148‑1 (MQ=255)
                                                                               tCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGACGTGGACCACTTCCTg                                                       <  5:154326/149‑1 (MQ=255)
                                                                                 aCCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGAGCTGAAATTTTTCCAGGAAGTGGTCGACGTCCTGcc                                                     >  7:13216/1‑149 (MQ=255)
                                                                                     gatgaGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGc                                                                                                       >  2:96611/1‑95 (MQ=255)
                                                                                     gatgaGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGc                                                                                                       <  1:96611/95‑1 (MQ=255)
                                                                                           tCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCttt                                           >  8:140104/1‑149 (MQ=255)
                                                                                            ccTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCTCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCttt                                           <  6:297753/148‑1 (MQ=255)
                                                                                             cTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGc                                         <  5:162013/149‑1 (MQ=255)
                                                                                             cTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGCCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGc                                         >  3:282974/1‑149 (MQ=255)
                                                                                                aaCTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGtt                                      <  2:198383/149‑1 (MQ=255)
                                                                                                  cTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGa                                    <  2:264238/149‑1 (MQ=255)
                                                                                                      aTGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCg                                 >  4:148953/1‑148 (MQ=255)
                                                                                                         gAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAg                              <  3:197550/148‑1 (MQ=255)
                                                                                                         gAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAg                              >  4:197550/1‑148 (MQ=255)
                                                                                                          aaCGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTcc                                                               >  5:207522/1‑114 (MQ=255)
                                                                                                          aaCGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTcc                                                               <  6:207522/114‑1 (MQ=255)
                                                                                                                  cTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGAt                    >  6:263748/1‑149 (MQ=255)
                                                                                                                     gAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGATGAAATTTTTCCAGGAATTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGAtg                   <  8:147117/147‑2 (MQ=255)
                                                                                                                       aaaCGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATCCTgg               <  1:145316/149‑1 (MQ=255)
                                                                                                                         gcgcgAGGCATACATCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATcc                  <  4:251237/143‑1 (MQ=255)
                                                                                                                         gcgcgAGGCATACATCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATcc                  >  3:251237/2‑144 (MQ=255)
                                                                                                                         aCGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATCCTGGt              >  3:303168/1‑148 (MQ=255)
                                                                                                                            cgATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATCCTGGTGATc          <  4:267855/149‑1 (MQ=255)
                                                                                                                                  aTGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATCCTGGTGATCCTGTCa    >  6:170489/1‑149 (MQ=255)
                                                                                                                                    gCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTc                                                          <  4:171176/93‑1 (MQ=255)
                                                                                                                                    gCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTc                                                          >  3:171176/1‑93 (MQ=255)
                                                                                                                                     ccTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATCCTGGTGATCCTGTCACt  >  5:144858/1‑148 (MQ=255)
                                                                                                                                      |                                                                                                                                                  
TGTCGCGATCAGGCTGCGGAGCAGCCCCCGGGCTTTTGCTGTAACAGGTGGCTGGCTATACTCGCCGTCGCTTTTTTAGTCACCTGATGAGTCCTGAACTTCATGGAACGTATCCTCGAAAACGCGATGTATGCCTCGCGCTGGCTGCTCGCACCGATCTACTTCGGCTTGTCCCTCGGCCTGCTGGCCCTGGCGCTGAAATTTTTCCAGGAAGTGGTCCACGTCCTGCCCAATGTCTTTGCGTTGAGCGAAGCTGACCTGATCCTGGTGATCCTGTCACT  >  NC_002947/796886‑797166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑