Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	797,509	G→A	9.3%	intergenic (+33/‑44)	PP_0682 → / → PP_0683	inner membrane protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	797,509	0	G	A	9.3%	176.4 / 6.8	64	intergenic (+33/‑44)	PP_0682/PP_0683	inner membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/26);  new base A (3/3);  total (35/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TCATGGATGCGCAGAACATCTCCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCATCAGCTCAA  >  NC_002947/797361‑797656                                                                                                                                                     |                                                                                                                                                     tCATGGATGCGCAGAACATCTCCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTgggg                                                                                                                                                      >  3:37471/1‑149 (MQ=255)   aTGGATGCGCAGAACATCTCCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTgggg                                                                                                                                                      >  7:60401/1‑147 (MQ=255)      gATGCGCAGAACATCTCCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGgggcggg                                                                                                                                                 <  1:187236/149‑1 (MQ=255)               aaCATCTCCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGc                                                                                                                                        <  6:41622/149‑1 (MQ=255)                  aTCTCCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTcc                                                                                                                                     >  5:78122/1‑149 (MQ=255)                   tctcCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTgggg                                                                                                                                                      >  6:102686/1‑131 (MQ=255)                   tctcCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTgggg                                                                                                                                                      <  5:102686/131‑1 (MQ=255)                     tcCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCca                                                                                                                                   >  8:130532/1‑148 (MQ=255)                           gATTACCTGATGTGGTAAGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑Gatat                                                                                                                           <  8:60401/149‑1 (MQ=255)                               agcTGTTGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑TATATCGa                                                                                                                        <  3:278281/146‑1 (MQ=255)                                ccTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAgg                                                                                                                      >  6:56117/1‑149 (MQ=255)                                ccTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAg                                                                                                                       <  1:54481/148‑1 (MQ=255)                                                 tcatcCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGc                                                                                                     <  6:78122/149‑1 (MQ=255)                                                 tcatcCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGc                                                                                                     <  7:130532/149‑1 (MQ=255)                                                   atcCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCgt                                                                                                   >  4:234900/1‑149 (MQ=255)                                                   atcCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCACACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCgt                                                                                                   >  2:225468/1‑149 (MQ=255)                                                   atcCACATGACCTTCGTGGTCTCGGCGTTATGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCgt                                                                                                   >  1:178889/1‑149 (MQ=255)                                                                     gTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGAc                                                                                                            >  1:116099/1‑122 (MQ=255)                                                                     gTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGAc                                                                                                            <  2:116099/122‑1 (MQ=255)                                                                     gTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCCAGGATCAACTGACGACTGGCATCAGTCAAGCCTTGCGCa                                                                                  >  5:305552/1‑148 (MQ=255)                                                                              ttCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTc                                                                        <  5:56117/149‑1 (MQ=255)                                                                                   cATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTccagct                                                                   >  1:207421/1‑145 (MQ=255)                                                                                   cATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTc                                                                        <  2:207421/144‑1 (MQ=255)                                                                                   cATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTa                                                                   >  1:39041/1‑149 (MQ=255)                                                                                   cATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCGTGCGCAGTCTCCCGTCCTGTa                                                                   >  8:171336/1‑149 (MQ=255)                                                                                      gggCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTc                                                                        >  4:278192/1‑141 (MQ=255)                                                                                      gggCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTc                                                                        <  3:278192/141‑1 (MQ=255)                                                                                      gggCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACa                                                                 >  5:256060/1‑148 (MQ=255)                                                                                          tACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACaaaaaa                                                            >  5:224133/1‑149 (MQ=255)                                                                                            ccTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAAt                                                           <  4:37471/148‑1 (MQ=255)                                                                                             cTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGa                                                         <  1:298484/149‑1 (MQ=255)                                                                                                     gCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTg                                                                     <  2:148394/129‑1 (MQ=255)                                                                                                     gCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTg                                                                     >  1:148394/1‑129 (MQ=255)                                                                                                     gCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCAt                                                 >  6:194363/1‑149 (MQ=255)                                                                                                     gCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCAt                                                 <  2:178889/149‑1 (MQ=255)                                                                                                     gCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCAt                                                 >  7:165509/1‑149 (MQ=255)                                                                                                       tGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGc                                               >  2:215521/1‑149 (MQ=255)                                                                                                         aCCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGt                                             >  5:12172/1‑149 (MQ=255)                                                                                                           cAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTgg                                           >  1:60705/1‑149 (MQ=255)                                                                                                            aaGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGTCGAGTGGCATCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGt                                          >  6:288273/1‑149 (MQ=255)                                                                                                             aGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCACTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGa                                                         >  5:110866/1‑133 (MQ=255)                                                                                                             aGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCACTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGa                                                         <  6:110866/133‑1 (MQ=255)                                                                                                               cACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTcc                                       <  7:171336/149‑1 (MQ=255)                                                                                                                 cTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCa                                     >  7:144086/1‑149 (MQ=255)                                                                                                                  tGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCag                                    >  4:237445/1‑148 (MQ=255)                                                                                                                  tGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCAt                                    >  1:278347/1‑149 (MQ=255)                                                                                                                    aGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATc                                   <  6:256060/148‑1 (MQ=255)                                                                                                                          cccAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGGATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGt                            >  3:236767/1‑149 (MQ=255)                                                                                                                              ggCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGctc                        <  2:39041/149‑1 (MQ=255)                                                                                                                                ccTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCtctc                      <  6:224133/149‑1 (MQ=255)                                                                                                                                 cTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACAGGATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGctct                       >  1:214464/1‑148 (MQ=255)                                                                                                                                 cTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCa                     <  1:215521/149‑1 (MQ=255)                                                                                                                                 cTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCa                     >  4:219807/1‑149 (MQ=255)                                                                                                                                      tGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAAc                >  5:91668/1‑149 (MQ=255)                                                                                                                                        cAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAg                              <  4:295536/133‑1 (MQ=255)                                                                                                                                        cAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAg                              >  3:295536/1‑133 (MQ=255)                                                                                                                                          aCTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGc            <  3:219807/149‑1 (MQ=255)                                                                                                                                          aCTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGc            <  8:165509/149‑1 (MQ=255)                                                                                                                                            tCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCAt          <  2:278347/149‑1 (MQ=255)                                                                                                                                            atcTGTGGGAGCGGGCATGCCCGCCCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCgtcag                                                                                                <  5:75553/61‑1 (MQ=25)                                                                                                                                            atcTGTGGGAGCGGGCATGCCCGCCCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCgtcag                                                                                                >  6:75553/3‑63 (MQ=25)                                                                                                                                              cTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCAt          >  3:115643/1‑147 (MQ=255)                                                                                                                                              cTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCATc         <  6:12172/148‑1 (MQ=255)                                                                                                                                              cTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCATCa        <  1:225468/149‑1 (MQ=255)                                                                                                                                              cTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCAAGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCAt          >  7:203376/1‑147 (MQ=255)                                                                                                                                               tgtgGGAGCGGGCATGCCCGCCCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCa                     <  7:95436/135‑1 (MQ=255)                                                                                                                                               tgtgGGAGCGGGCATGCCCGCCCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCa                     >  8:95436/1‑135 (MQ=255)                                                                                                                                               tgtgGGAGCGGGCATGCCCGCCCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCa                     >  8:90920/1‑135 (MQ=255)                                                                                                                                               tgtgGGAGCGGGCATGCCCGCCCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCa                     <  7:90920/135‑1 (MQ=255)                                                                                                                                                   ggggCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCATCAGCTc    >  6:17947/1‑148 (MQ=255)                                                                                                                                                    gggcgggcATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCAAGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCATCAGCTCaa  <  8:203376/149‑1 (MQ=255)                                                                                                                                                     ggcgggcATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGtt                                         >  8:11996/1‑109 (MQ=255)                                                                                                                                                     ggcgggcATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGtt                                         <  7:11996/109‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TCATGGATGCGCAGAACATCTCCACCGATTACCTGATGTGGTACGTGATCATCCACATGACCTTCGTGGTCTCGGCGTTCTGCATGGGCTACCTGGACAAGCTGACCAAGCACTGAGTCCCCCCAGGCCTCACTGCAACTCCTGTGGGGGCGGGCATGCCCGCTCCCACA‑GATATCGAGGCTCAACTGACGAGTGGCGTCAGTCATGCCTTGCGCATTCTCCCTTCCTGTACAAAAAATGAGCTCTCATGCGTGGTTCCCATCGCGAGGTGCTCTCATGAACCTGCATCAGCTCAA  >  NC_002947/797361‑797656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑