Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 797,983 T→A 7.9% intergenic (+41/‑45) PP_0683 → / → fklB‑I hypothetical protein/FKBP‑type peptidyl‑prolyl cis‑trans isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947797,9830TA 7.9% 170.8 / 2.9 59intergenic (+41/‑45)PP_0683/fklB‑Ihypothetical protein/FKBP‑type peptidyl‑prolyl cis‑trans isomerase
Reads supporting (aligned to +/- strand):  ref base T (30/24);  new base A (3/2);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TCTCGATGAACCTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAG  >  NC_002947/797836‑798128
                                                                                                                                                   |                                                                                                                                                 
tctcGATGAACCTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCttttt                                                                                                                                                  <  6:17275/149‑1 (MQ=255)
         aCCTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGgc                                                                                                                                         >  8:211808/1‑149 (MQ=255)
           cTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTGATAAAAAGGgcga                                                                                                                                       <  2:284817/149‑2 (MQ=255)
              gTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCgg                                                                                                                                     <  2:290014/148‑1 (MQ=255)
                          gTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGccc                                                                                                                        <  8:268731/149‑1 (MQ=255)
                           tGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCt                                                                                                                       >  3:105574/1‑149 (MQ=255)
                                gACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTGATAAAAAGGgcgag                                                                                                                                      >  8:243166/1‑127 (MQ=255)
                                gACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTGATAAAAAGGgcgag                                                                                                                                      <  7:243166/129‑3 (MQ=255)
                                    aaaTGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTTGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAg                                                                                                              <  5:51290/149‑1 (MQ=255)
                                      aTGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATCGCGCCCTGCAGTGGAGCa                                                                                                            <  7:211808/149‑1 (MQ=255)
                                                  ggTACGCACCCGGAAGAAGACCTGAAGGGGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTcc                                                                                                 <  4:243506/148‑1 (MQ=255)
                                                      cGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCt                                                                                                                       >  8:94089/1‑122 (MQ=255)
                                                      cGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCt                                                                                                                       <  7:94089/122‑1 (MQ=255)
                                                        cACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCa                                                                                          <  1:31198/149‑1 (MQ=255)
                                                          cccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAAc                                                                                        <  1:166522/149‑1 (MQ=255)
                                                          cccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAAc                                                                                        >  2:166522/1‑149 (MQ=255)
                                                          cccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGAGGAGCACGACAATTTCAGACCTCAAc                                                                                        >  8:261572/1‑149 (MQ=255)
                                                           ccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAg                                                                                                                                            >  5:113586/1‑96 (MQ=255)
                                                           ccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAg                                                                                                                                            <  6:113586/96‑1 (MQ=255)
                                                              gaagaaGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGACCTCACCCTgg                                                                                    >  7:103722/1‑148 (MQ=255)
                                                              gaagaaGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTg                                                                                     >  7:99379/1‑148 (MQ=255)
                                                              gaagaaGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGt                                                                                    <  3:134261/149‑1 (MQ=255)
                                                                 gaagaCCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTa                                                                                 >  8:259342/1‑149 (MQ=255)
                                                                       cTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGAGAATGTCCGAACTCAACCTGTCTACcgac                                                                            >  2:138571/1‑148 (MQ=255)
                                                                                        ccAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCTAACTCAACCTGTCTACCGACGaatacc                                                                     >  8:257751/1‑134 (MQ=255)
                                                                                         cAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACg                                                         >  6:56137/1‑149 (MQ=255)
                                                                                                   ccTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCg                                               >  4:222987/1‑149 (MQ=255)
                                                                                                       gTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAg                                           >  4:119123/1‑149 (MQ=255)
                                                                                                       gTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAg                                           <  5:161859/149‑1 (MQ=255)
                                                                                                           ctgctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTCCCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTgg                                       <  5:158300/149‑1 (MQ=255)
                                                                                                           ctgctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTgg                                       <  5:56137/149‑1 (MQ=255)
                                                                                                            tgctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCACCCTGTCTACCGCCGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTggg                                      >  6:230865/1‑149 (MQ=255)
                                                                                                             gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCt                                                                                      <  2:109903/100‑1 (MQ=255)
                                                                                                             gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCt                                                                                      >  1:109903/1‑100 (MQ=255)
                                                                                                             gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGt                                     >  5:274454/1‑149 (MQ=255)
                                                                                                             gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGt                                     >  3:256067/1‑149 (MQ=255)
                                                                                                               tCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTgg                                   >  4:222214/1‑149 (MQ=255)
                                                                                                                 gCAGCGCCCGAGTGTGATAGGCTGCTCGCCCTTTTTATCCAGGGCGCGGCTGCATTGCGCCCTGCAGGGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGcc                                 <  7:261572/149‑1 (MQ=255)
                                                                                                                 gCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGc                                  <  4:256067/148‑1 (MQ=255)
                                                                                                                         ccAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                         <  3:222987/149‑1 (MQ=255)
                                                                                                                         ccAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                         >  1:251651/1‑149 (MQ=255)
                                                                                                                         ccAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                         <  4:105574/149‑1 (MQ=255)
                                                                                                                          cAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                         >  3:121284/1‑148 (MQ=255)
                                                                                                                            gtgtGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGa                       >  4:75567/1‑148 (MQ=255)
                                                                                                                               tgCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAAc                   >  3:249164/1‑149 (MQ=255)
                                                                                                                                 cTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGcc                                                >  4:254387/1‑118 (MQ=255)
                                                                                                                                 cTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGcc                                                <  3:254387/118‑1 (MQ=255)
                                                                                                                                  tAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCg                >  4:71680/1‑149 (MQ=255)
                                                                                                                                  tAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCg                >  8:121830/1‑149 (MQ=255)
                                                                                                                                     gctgctCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCAACAACCCGcca             <  7:259342/149‑1 (MQ=255)
                                                                                                                                       gccgcGCCCTTGATAAAAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGGGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGccac            >  1:226448/5‑148 (MQ=255)
                                                                                                                                        gctCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGccac            >  5:23928/1‑147 (MQ=255)
                                                                                                                                        ccgcGCCCTTGATAAAAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGccac            >  1:77427/4‑147 (MQ=255)
                                                                                                                                           cGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc       <  3:119123/149‑1 (MQ=255)
                                                                                                                                            gCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc       <  8:151860/148‑1 (MQ=255)
                                                                                                                                            gCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc       <  2:251651/148‑1 (MQ=255)
                                                                                                                                            gCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc       >  7:151860/1‑148 (MQ=255)
                                                                                                                                              ccTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGt     >  6:242874/1‑148 (MQ=255)
                                                                                                                                               cTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGt     >  3:32478/1‑147 (MQ=255)
                                                                                                                                               cTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTa   <  4:121284/149‑1 (MQ=255)
                                                                                                                                                tttttATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAg  >  7:189097/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCTCGATGAACCTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAG  >  NC_002947/797836‑798128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑