Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 797,986 T→A 8.3% intergenic (+44/‑42) PP_0683 → / → fklB‑I hypothetical protein/FKBP‑type peptidyl‑prolyl cis‑trans isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947797,9860TA 8.3% 176.2 / 4.5 60intergenic (+44/‑42)PP_0683/fklB‑Ihypothetical protein/FKBP‑type peptidyl‑prolyl cis‑trans isomerase
Reads supporting (aligned to +/- strand):  ref base T (31/24);  new base A (3/2);  total (34/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

ACCTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAGCCTGGA  >  NC_002947/797845‑798134
                                                                                                                                             |                                                                                                                                                    
aCCTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGgc                                                                                                                                               >  8:211808/1‑149 (MQ=255)
  cTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTGATAAAAAGGgcga                                                                                                                                             <  2:284817/149‑2 (MQ=255)
     gTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCgg                                                                                                                                           <  2:290014/148‑1 (MQ=255)
                 gTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGccc                                                                                                                              <  8:268731/149‑1 (MQ=255)
                  tGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCt                                                                                                                             >  3:105574/1‑149 (MQ=255)
                       gACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTGATAAAAAGGgcgag                                                                                                                                            >  8:243166/1‑127 (MQ=255)
                       gACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTGATAAAAAGGgcgag                                                                                                                                            <  7:243166/129‑3 (MQ=255)
                           aaaTGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTTGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAg                                                                                                                    <  5:51290/149‑1 (MQ=255)
                             aTGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATCGCGCCCTGCAGTGGAGCa                                                                                                                  <  7:211808/149‑1 (MQ=255)
                                         ggTACGCACCCGGAAGAAGACCTGAAGGGGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTcc                                                                                                       <  4:243506/148‑1 (MQ=255)
                                             cGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCt                                                                                                                             >  8:94089/1‑122 (MQ=255)
                                             cGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCt                                                                                                                             <  7:94089/122‑1 (MQ=255)
                                               cACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCa                                                                                                <  1:31198/149‑1 (MQ=255)
                                                 cccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAAc                                                                                              <  1:166522/149‑1 (MQ=255)
                                                 cccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAAc                                                                                              >  2:166522/1‑149 (MQ=255)
                                                 cccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGAGGAGCACGACAATTTCAGACCTCAAc                                                                                              >  8:261572/1‑149 (MQ=255)
                                                  ccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAg                                                                                                                                                  >  5:113586/1‑96 (MQ=255)
                                                  ccGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAg                                                                                                                                                  <  6:113586/96‑1 (MQ=255)
                                                     gaagaaGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGACCTCACCCTgg                                                                                          >  7:103722/1‑148 (MQ=255)
                                                     gaagaaGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTg                                                                                           >  7:99379/1‑148 (MQ=255)
                                                     gaagaaGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGt                                                                                          <  3:134261/149‑1 (MQ=255)
                                                        gaagaCCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTa                                                                                       >  8:259342/1‑149 (MQ=255)
                                                              cTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGAGAATGTCCGAACTCAACCTGTCTACcgac                                                                                  >  2:138571/1‑148 (MQ=255)
                                                                               ccAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCTAACTCAACCTGTCTACCGACGaatacc                                                                           >  8:257751/1‑134 (MQ=255)
                                                                                cAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACg                                                               >  6:56137/1‑149 (MQ=255)
                                                                                          ccTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCg                                                     >  4:222987/1‑149 (MQ=255)
                                                                                              gTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAg                                                 >  4:119123/1‑149 (MQ=255)
                                                                                              gTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAg                                                 <  5:161859/149‑1 (MQ=255)
                                                                                                  ctgctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTCCCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTgg                                             <  5:158300/149‑1 (MQ=255)
                                                                                                  ctgctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTgg                                             <  5:56137/149‑1 (MQ=255)
                                                                                                   tgctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCACCCTGTCTACCGCCGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTggg                                            >  6:230865/1‑149 (MQ=255)
                                                                                                    gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCt                                                                                            <  2:109903/100‑1 (MQ=255)
                                                                                                    gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCt                                                                                            >  1:109903/1‑100 (MQ=255)
                                                                                                    gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGt                                           >  3:256067/1‑149 (MQ=255)
                                                                                                    gctCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGt                                           >  5:274454/1‑149 (MQ=255)
                                                                                                      tCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTgg                                         >  4:222214/1‑149 (MQ=255)
                                                                                                        gCAGCGCCCGAGTGTGATAGGCTGCTCGCCCTTTTTATCCAGGGCGCGGCTGCATTGCGCCCTGCAGGGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGcc                                       <  7:261572/149‑1 (MQ=255)
                                                                                                        gCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGc                                        <  4:256067/148‑1 (MQ=255)
                                                                                                                ccAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                               <  3:222987/149‑1 (MQ=255)
                                                                                                                ccAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                               >  1:251651/1‑149 (MQ=255)
                                                                                                                ccAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                               <  4:105574/149‑1 (MQ=255)
                                                                                                                 cAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTgcgc                               >  3:121284/1‑148 (MQ=255)
                                                                                                                   gtgtGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGa                             >  4:75567/1‑148 (MQ=255)
                                                                                                                      tgCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAAc                         >  3:249164/1‑149 (MQ=255)
                                                                                                                        cTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGcc                                                      >  4:254387/1‑118 (MQ=255)
                                                                                                                        cTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGcc                                                      <  3:254387/118‑1 (MQ=255)
                                                                                                                         tAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCg                      >  4:71680/1‑149 (MQ=255)
                                                                                                                         tAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCg                      >  8:121830/1‑149 (MQ=255)
                                                                                                                            gctgctCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCAACAACCCGcca                   <  7:259342/149‑1 (MQ=255)
                                                                                                                              gccgcGCCCTTGATAAAAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGGGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGccac                  >  1:226448/5‑148 (MQ=255)
                                                                                                                               gctCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGccac                  >  5:23928/1‑147 (MQ=255)
                                                                                                                               ccgcGCCCTTGATAAAAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGccac                  >  1:77427/4‑147 (MQ=255)
                                                                                                                                  cGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc             <  3:119123/149‑1 (MQ=255)
                                                                                                                                   gCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc             >  7:151860/1‑148 (MQ=255)
                                                                                                                                   gCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc             <  2:251651/148‑1 (MQ=255)
                                                                                                                                   gCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGc             <  8:151860/148‑1 (MQ=255)
                                                                                                                                     ccTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGt           >  6:242874/1‑148 (MQ=255)
                                                                                                                                      cTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGt           >  3:32478/1‑147 (MQ=255)
                                                                                                                                      cTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTa         <  4:121284/149‑1 (MQ=255)
                                                                                                                                       tttttATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAg        >  7:189097/1‑149 (MQ=255)
                                                                                                                                             tCAATGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAAc                         <  5:106541/126‑1 (MQ=255)
                                                                                                                                             tCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAGCCTgg   >  5:157983/1‑148 (MQ=255)
                                                                                                                                             tCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAGCCTGGa  <  3:75567/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
ACCTGGTGCACTGGTCGGTGCAGGACGAAATGTGCGGCATGGGTACGCACCCGGAAGAAGACCTGAAGGTGCCGATTTCCCAGCGTTCGGCCTGGTAGCTGCTCGCAGCGCCCCAGTGTGCTAGGCTGCTCGCCCTTTTTATCAAGGGCGCGGCTGCATTGCGCCCTGCAGTGGAGCACGACAATGTCCGAACTCAACCTGTCTACCGACGAAACGCGCGTCAGCTACGGTATCGGCCGTCAGCTGGGTGGCCAGCTGCGCGACAACCCGCCACCAGGCGTTAGCCTGGA  >  NC_002947/797845‑798134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑