Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 828,921 C→G 7.7% T178S (ACT→AGT)  kefB‑I → CPA2 family Kef‑type potassium/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947828,9210CG 7.7% 152.8 / 4.1 52T178S (ACT→AGT) kefB‑ICPA2 family Kef‑type potassium/proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (25/22);  new base G (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

TGGCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCT  >  NC_002947/828784‑829065
                                                                                                                                          |                                                                                                                                                 
tGGCTTGCAGGTACTGGTCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGAcatc                                                                                                                                         <  2:230512/148‑4 (MQ=255)
tGGCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCAGTGCTGAcat                                                                                                                                          <  7:261802/147‑3 (MQ=255)
       cAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTg                                                                                                                                 <  5:172730/149‑1 (MQ=255)
         ggTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGc                                                                                                                               >  4:150943/1‑149 (MQ=255)
                gCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTgct                                                                                                                        >  4:198800/1‑149 (MQ=255)
                     gcgcAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTgctggctg                                                                                                                   <  1:52850/149‑1 (MQ=255)
                       gcAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCtgtg                                                                                                                 <  1:106776/149‑1 (MQ=255)
                             aCCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTc                                                                                                           <  6:255720/149‑1 (MQ=255)
                               cTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCgg                                                                                                         >  5:118946/1‑149 (MQ=255)
                               cTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCATGCTGGCTGTGGTTGTCgg                                                                                                         >  2:236956/1‑149 (MQ=255)
                                   gCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCAtt                                                                                                     <  4:7723/149‑1 (MQ=255)
                                       aCCTAATGGCCGCATGGCCGTGGCCATCCTTGTGTTCCAGTAAATTTCCCCCATTCCCTTTTTTTGCGTGGTGGCCGTGCTCGGGGGTTTATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGgt                                                                                                  <  1:87323/149‑1 (MQ=255)
                                            atggccgcatggccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCt                                                                                             >  2:62583/1‑148 (MQ=255)
                                              ggccgcatggccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGAt                                                                                          >  6:299280/1‑149 (MQ=255)
                                                        ccgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑c                                                                               >  4:76771/1‑149 (MQ=255)
                                                         cgTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑cc                                                                              <  6:118946/149‑1 (MQ=255)
                                                             gCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTa                                                                          >  8:85090/1‑149 (MQ=255)
                                                               cATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATc                                                                        >  4:298813/1‑149 (MQ=255)
                                                                aTCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGtgtg                                                                                               <  5:43061/126‑1 (MQ=255)
                                                                aTCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGtgtg                                                                                               >  6:43061/1‑126 (MQ=255)
                                                                    tgctgTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATctgct                                                                    >  5:278306/1‑148 (MQ=255)
                                                                            cAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGa                                                            >  5:280811/1‑148 (MQ=255)
                                                                                aCATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTc                                                       <  3:298813/149‑1 (MQ=255)
                                                                                  aTTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCaa                                                     <  6:280811/149‑1 (MQ=255)
                                                                                  aTTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCaa                                                     <  7:85090/149‑1 (MQ=255)
                                                                                     gccgccATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGt                                                   <  6:278306/148‑1 (MQ=255)
                                                                                     gccgccATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGt                                                   <  3:150943/148‑1 (MQ=255)
                                                                                        gccATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGata                                                          >  1:22402/1‑138 (MQ=255)
                                                                                        gccATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGata                                                          <  2:22402/138‑1 (MQ=255)
                                                                                              cccTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTggg                                         >  6:95259/1‑149 (MQ=255)
                                                                                               ccTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGatat                                                         >  1:221583/1‑132 (MQ=255)
                                                                                               ccTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGatat                                                         <  2:221583/132‑1 (MQ=255)
                                                                                               ccTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGt                                        >  2:157990/1‑149 (MQ=255)
                                                                                                   gATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCgg                                    >  8:34393/1‑149 (MQ=255)
                                                                                                    aTTGCCGTGGTGCCGCTGGTCGGTGG‑GGTTGTCATCAATTCTGTAGTAGGGGCCTGGCCCTTGCTGGGTGGGGTTGTCGGCATTGGGGGGCTGATTGTTTTTGGTCCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCgg                                    <  1:62583/149‑1 (MQ=255)
                                                                                                     ttGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgc                                  <  1:157990/149‑1 (MQ=255)
                                                                                                        ccGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATCGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcct                                <  3:198800/148‑1 (MQ=255)
                                                                                                        ccGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCATGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcctg                               <  1:236956/149‑1 (MQ=255)
                                                                                                            ggtgCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcctgcct                            >  3:256106/1‑148 (MQ=255)
                                                                                                            ggtgCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGgcctgcct                            >  7:124170/1‑148 (MQ=255)
                                                                                                                  gctgctCGGTGG‑CGATCTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGt                     >  4:62530/1‑149 (MQ=255)
                                                                                                                    tgctCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTc                    >  2:238084/1‑148 (MQ=255)
                                                                                                                      ctCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGACATTGGTGTGCTGATTGTTTTTGG‑CCg                                                                             <  2:102309/89‑1 (MQ=255)
                                                                                                                          gTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGt                                                                            >  7:105115/1‑86 (MQ=255)
                                                                                                                          gTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGt                                                                            <  8:105115/86‑1 (MQ=255)
                                                                                                                          gTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAAc                        <  4:133817/138‑1 (MQ=255)
                                                                                                                          gTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAAc                        >  3:133817/1‑138 (MQ=255)
                                                                                                                               cttcgTCAGCAGTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGACACgg         >  7:123124/5‑149 (MQ=255)
                                                                                                                                ttcgTCAGCAGTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGc        >  7:253819/4‑149 (MQ=255)
                                                                                                                                     cAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGAccagc    >  6:92448/1‑145 (MQ=255)
                                                                                                                                      aGCAGTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGc   <  8:123124/148‑1 (MQ=255)
                                                                                                                                      aGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCt  >  4:174169/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                 
TGGCTTGCAGGTACTGGCCGAGCGCAAGGACCTGGGCAAACCTCATGGCCGCATGGCCGTGGCCATCCTGCTGTTCCAGGACATTGCCGCCATTCCCTTGATTGCCGTGGTGCCGCTGCTCGGTGG‑CGATGTCAGCACTGCTGACGAAGGTGCCTGGCCCTTGCTGGCTGTGGTTGTCGGCATTGGTGTGCTGATTGTTTTTGG‑CCGTTATCTGCTGACCCCGATATTCAAGTGGACGGTGGGTTCGGGCCTGCCTGAACTGTCGACCGCCACGGCCTTGCT  >  NC_002947/828784‑829065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑