Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 848,503 G→A 7.8% L23F (CTT→TTT)  PP_0730 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947848,5030GA 7.8% 146.4 / 2.2 51L23F (CTT→TTT) PP_0730hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

AATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGTGCG  >  NC_002947/848361‑848651
                                                                                                                                              |                                                                                                                                                    
aaTTCGCCGCCTTCCAGATTATCGCTACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGttt                                                                                                                                                <  1:99905/149‑1 (MQ=255)
aaTTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGttt                                                                                                                                                >  5:114117/1‑149 (MQ=255)
   tCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGc                                                                                                                                             >  4:269/1‑149 (MQ=255)
         ccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAACGGCTTGGCTGccac                                                                                                                                       <  4:11587/149‑3 (MQ=255)
           ttCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCa                                                                                                                                      >  6:168315/1‑148 (MQ=255)
              cAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCaaaa                                                                                                                                   <  1:160057/148‑1 (MQ=255)
                     tCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCcagcag                                                                                                                           >  6:237898/1‑149 (MQ=255)
                        aTACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGcc                                                                                                                        <  7:18337/149‑1 (MQ=255)
                            gCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCtt                                                                                                                    <  5:168315/149‑1 (MQ=255)
                                   aGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTg                                                                                                              <  4:133719/148‑1 (MQ=255)
                                          acaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcgac                                                                                                      >  2:48783/1‑149 (MQ=255)
                                          acaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcgac                                                                                                      >  2:53361/1‑149 (MQ=255)
                                                cGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAAc                                                                                                >  5:274074/1‑149 (MQ=255)
                                                  cAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCa                                                                                              <  1:293532/149‑1 (MQ=255)
                                                   aGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCAc                                                                                             <  3:269/149‑1 (MQ=255)
                                                     gTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTg                                                                                                              >  6:131126/1‑130 (MQ=255)
                                                     gTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTg                                                                                                              <  5:131126/130‑1 (MQ=255)
                                                          gCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGttt                                                                                       >  8:155758/1‑148 (MQ=255)
                                                                 aCGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGaa                                                                               <  6:114117/149‑1 (MQ=255)
                                                                    ggAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGcc                                                                                                                                                    <  2:222157/77‑1 (MQ=255)
                                                                    ggAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGcc                                                                                                                                                    >  1:222157/1‑77 (MQ=255)
                                                                        gtgtCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTAc                                                                                                                  >  5:142674/1‑107 (MQ=255)
                                                                        gtgtCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTAc                                                                                                                  <  6:142674/107‑1 (MQ=255)
                                                                          gtcgtcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGAt                                                                       >  5:222658/1‑148 (MQ=255)
                                                                          gtcgtcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGAt                                                                       >  5:278868/1‑148 (MQ=255)
                                                                           tcgtcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGAtt                                                                      <  6:222658/148‑1 (MQ=255)
                                                                                cTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAgg                                                                >  6:58855/1‑149 (MQ=255)
                                                                                cTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAg                                                                 <  3:77935/148‑1 (MQ=255)
                                                                                   gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGa                                                              <  3:168884/148‑1 (MQ=255)
                                                                                    aTCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTCGAACCTCGGATTACCAGGGAt                                                             >  3:112744/1‑148 (MQ=255)
                                                                                         gaTCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAg                                                        >  1:295086/1‑148 (MQ=255)
                                                                                             gTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTaaa                                                    <  7:155758/148‑1 (MQ=255)
                                                                                             gTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTAGACGAAAACCACGTGTTTCAtt                                                                                   <  7:47081/117‑1 (MQ=255)
                                                                                                 ggAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTg                                               <  6:278868/149‑1 (MQ=255)
                                                                                                 ggAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTg                                               <  1:53361/149‑1 (MQ=255)
                                                                                                 ggAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTg                                               <  1:48783/149‑1 (MQ=255)
                                                                                                  gAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGc                                              >  4:251858/1‑149 (MQ=255)
                                                                                                  gAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGc                                              >  6:78146/1‑149 (MQ=255)
                                                                                                  gAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGc                                              <  5:237898/149‑1 (MQ=255)
                                                                                                                      gaatgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGc                           >  8:179331/1‑148 (MQ=255)
                                                                                                                        atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCg                          >  1:173166/1‑147 (MQ=255)
                                                                                                                        atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                        <  3:251858/149‑1 (MQ=255)
                                                                                                                            aGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagcaa                    <  2:173166/149‑1 (MQ=255)
                                                                                                                              gTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagcaagc                  >  3:302833/1‑149 (MQ=255)
                                                                                                                                  cGTGGCAGCAAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGAc               <  8:167199/148‑1 (MQ=255)
                                                                                                                                   gTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGAAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGAcc              <  6:274074/148‑1 (MQ=255)
                                                                                                                                    aggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGt            >  5:63871/2‑149 (MQ=255)
                                                                                                                                     ggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGaaa                                                         <  2:260337/103‑1 (MQ=255)
                                                                                                                                     ggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGaaa                                                         >  1:260337/1‑103 (MQ=255)
                                                                                                                                            aaGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagc                      >  4:280917/1‑131 (MQ=255)
                                                                                                                                            aaGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagc                      <  3:280917/131‑1 (MQ=255)
                                                                                                                                              gCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGTgcg  >  2:144730/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
AATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGTGCG  >  NC_002947/848361‑848651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑