Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	875,283	A→C	6.5%	intergenic (+22/+24)	PP_0759 → / ← PP_0760	hypothetical protein/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	875,283	0	A	C	6.5%	190.8 / 4.2	62	intergenic (+22/+24)	PP_0759/PP_0760	hypothetical protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base A (27/31);  new base C (2/2);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

AGGCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAA  >  NC_002947/875137‑875423                                                                                                                                                   |                                                                                                                                              aGGCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATg                                                                                                                                             >  8:216506/1‑149 (MQ=255)  ggCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCAGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATgg                                                                                                                                            >  4:152685/1‑149 (MQ=255)   gCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGc                                                                                                                                           >  8:11345/1‑149 (MQ=255)       ccGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGcc                                                                                                                                       >  6:259624/1‑149 (MQ=255)         gAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGcccc                                                                                                                                     >  3:73135/1‑149 (MQ=255)          aaGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCCATGCAGcccca                                                                                                                                    <  7:155490/149‑2 (MQ=255)            ggTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGccccc‑cc                                                                                                                                 <  4:120116/149‑1 (MQ=255)                cACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCAGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGtt                                                                                                                             <  3:152685/149‑1 (MQ=255)                    gCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTcc                                                                                                                         <  3:41937/149‑1 (MQ=255)                     ccTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTccc                                                                                                                        <  7:216506/149‑1 (MQ=255)                         aGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCtt                                                                                                                      <  1:28006/147‑1 (MQ=255)                         aGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTa                                                                                                                    <  5:122784/149‑1 (MQ=255)                          gTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTa                                                                                                                    <  5:259624/148‑1 (MQ=255)                          gTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTAc                                                                                                                   <  3:184419/149‑1 (MQ=255)                           ttCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTAcc                                                                                                                  <  7:11345/149‑1 (MQ=255)                            tCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTAcc                                                                                                                  <  4:55979/148‑1 (MQ=255)                            tCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTAcc                                                                                                                  >  8:221036/1‑148 (MQ=255)                              ataaGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCaa                                                                                                               >  6:82624/4‑149 (MQ=255)                              aTGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCaa                                                                                                               <  4:73135/149‑1 (MQ=255)                               tGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAAt                                                                                                              >  4:181189/1‑149 (MQ=255)                                gAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATc                                                                                                             >  2:193028/1‑149 (MQ=255)                                   tACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGGTTTCCCTTTACCCAATCCAg                                                                                                          >  5:206058/1‑149 (MQ=255)                                      tCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCATGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCa                                                                                                       >  5:186718/1‑149 (MQ=255)                                        aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTAc                                                                                                                   <  3:271531/135‑1 (MQ=255)                                        aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTAc                                                                                                                   >  4:271531/1‑135 (MQ=255)                                        aCCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACt                                                                                                     >  1:54047/1‑149 (MQ=255)                                          ccGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTgg                                                                                                   >  3:262520/1‑149 (MQ=255)                                           cGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCCACCGGTTTTCCCTTTACCCAATCCAGCCACTgg                                                                                                   <  1:193028/149‑1 (MQ=255)                                               gTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTc                                                                                              <  7:221036/149‑1 (MQ=255)                                                 ccGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCAt                                                                                            >  8:43218/1‑149 (MQ=255)                                                   gcgAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTg                                                                                          >  1:165792/1‑149 (MQ=255)                                                    cgAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGc                                                                                         >  8:210415/1‑149 (MQ=255)                                                        ccGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGa                                                                                      <  7:43218/148‑1 (MQ=255)                                                            aTTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGa                                                                                 >  2:93980/1‑149 (MQ=255)                                                             ttGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGAc                                                                                <  1:93980/149‑1 (MQ=255)                                                               gCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACg                                                                               <  2:165792/148‑1 (MQ=255)                                                                   cGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGcc                                                                            <  5:82624/147‑1 (MQ=255)                                                                     cTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGa                                                                        <  3:181189/149‑1 (MQ=255)                                                                      tGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGAt                                                                       >  5:11259/1‑149 (MQ=255)                                                                        cAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGAtt                                                                      <  6:11259/148‑1 (MQ=255)                                                                             ggcgATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCAtt                                                                                           >  5:28754/1‑122 (MQ=255)                                                                             ggcgATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCAtt                                                                                           <  6:28754/122‑1 (MQ=255)                                                                                gATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTAc                                                              >  5:68479/1‑148 (MQ=255)                                                                                   tCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACg                                                             >  5:211451/1‑146 (MQ=255)                                                                                     cGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCCATGCAGcccca                                                                                                                                    >  8:192153/1‑73 (MQ=39)                                                                                     cGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCCATGCAGcccca                                                                                                                                    <  7:192153/74‑2 (MQ=39)                                                                                       aaGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTg                                                                                                    <  7:268013/103‑1 (MQ=255)                                                                                       aaGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTg                                                                                                    >  8:268013/1‑103 (MQ=255)                                                                                                  gCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGTCACGATTGCCAGTACGAAGAACGCCGTTgccgcc                                           <  2:166541/149‑1 (MQ=255)                                                                                                   cTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCa                                          <  6:68479/149‑1 (MQ=255)                                                                                                   cTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCa                                          <  3:48543/149‑1 (MQ=255)                                                                                                     gTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAgg                                        >  4:230870/1‑149 (MQ=255)                                                                                                        tCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcgg                                     <  3:258941/149‑1 (MQ=255)                                                                                                        tCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAggcg                                      <  6:186718/148‑1 (MQ=255)                                                                                                                 cgccAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGc                            <  7:210415/149‑1 (MQ=255)                                                                                                                  gccAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGcc                                                    >  2:67611/1‑124 (MQ=255)                                                                                                                  gccAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGcc                                                    <  1:67611/124‑1 (MQ=255)                                                                                                                  gccAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCg                           >  2:195566/1‑149 (MQ=255)                                                                                                                         cGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACgg                    <  4:262520/149‑1 (MQ=255)                                                                                                                                       tCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCcg      >  3:115647/1‑149 (MQ=255)                                                                                                                                        gggggggCTGCCATGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCcgc     >  1:101136/4‑149 (MQ=255)                                                                                                                                            gggCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTCCCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGaa  <  3:7470/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                              AGGCTTCCGAAGGTGCACCGCCTGAGTTCATGAGTACTCACCCGGCGTCCGCGAGCCGCATTGCTTCGCTGCAGGCGGCGATTCCGAAGGTGATGCCGCTGTATCAGGCTGCCGCCAAGCCGTAAATGCCTGAATCCTGGGGCTGCATGGCAGCCCCC‑CCGGTTTTCCCTTTACCCAATCCAGCCACTGGCCTTCATTGCCGAATAGACGGCCACGATTGCCAGTACGAAGAACGCCGTTGCCGCCAGGCGGCGAATCAGCGTCAACGGCAGTTTGTCTGCCGCGAA  >  NC_002947/875137‑875423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑