Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 913,036 T→C 6.6% F231L (TTC→CTC)  fruK → 1‑phosphofructokinase monomer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947913,0360TC 6.6% 167.0 / 2.1 61F231L (TTC→CTC) fruK1‑phosphofructokinase monomer
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base C (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCCAATACCGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGACCCGGGCCACTGCCA  >  NC_002947/912896‑913183
                                                                                                                                            |                                                                                                                                                   
aGCCCAATACCGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTcgc                                                                                                                                             <  8:177761/149‑1 (MQ=255)
 gCCCAATACCGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTcgc                                                                                                                                             >  2:128748/1‑148 (MQ=255)
    cAATACCGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATc                                                                                                                                         >  3:17346/1‑149 (MQ=255)
         ccGACGAACTGGGCGACGTCCTGCTCCTGGCTGTGGATAACCTGAGGCAACAGCGCGCTGCCGCCACGCGCCTGCTGGACAGTGGCGATGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGTTTCAGTCGCGATCTGGcc                                                                                                                                    <  8:277950/149‑1 (MQ=255)
          cGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGAt                                                                                                                                          <  7:28676/142‑1 (MQ=255)
          cGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGAt                                                                                                                                          >  8:28676/1‑142 (MQ=255)
          cGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGccc                                                                                                                                   >  2:79090/1‑149 (MQ=255)
          cGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGccc                                                                                                                                   >  3:146528/1‑149 (MQ=255)
           gaagaaCTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCt                                                                                                                                  >  7:165338/1‑149 (MQ=255)
                  tGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGgcc                                                                                                                           >  6:275481/1‑149 (MQ=255)
                    ggCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTc                                                                                                                                               >  2:114336/1‑127 (MQ=255)
                    ggCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTc                                                                                                                                               <  1:114336/127‑1 (MQ=255)
                    ggCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTc                                                                                                                                               >  6:226613/1‑127 (MQ=255)
                    ggCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTc                                                                                                                                               <  5:226613/127‑1 (MQ=255)
                       gAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGgcccgccc                                                                                                                      >  2:124284/1‑149 (MQ=255)
                               gggCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAgg                                                                                                              >  8:129230/1‑149 (MQ=255)
                                  ccTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGc                                                                                                           <  1:79090/149‑1 (MQ=255)
                                      gCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCgg                                                                                                         <  1:128748/147‑1 (MQ=255)
                                       cTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTgg                                                                                                                                      <  7:229342/117‑1 (MQ=255)
                                       cTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTgg                                                                                                                                      >  8:229342/1‑117 (MQ=255)
                                               aaCCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGCTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCAc                                                                                              >  7:57920/1‑149 (MQ=255)
                                               aaCCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGCTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCAc                                                                                              >  7:57930/1‑149 (MQ=255)
                                                           cAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCggg                                                                                  <  8:165338/149‑1 (MQ=255)
                                                                     ccgccAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTGCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCtg                                                                        <  8:181520/149‑1 (MQ=255)
                                                                     ccgccAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGGGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGAGCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGc                                                                          <  7:127972/147‑1 (MQ=255)
                                                                      cgccAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCtgg                                                                       >  3:91236/1‑149 (MQ=255)
                                                                       gccAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCtggt                                                                      >  7:42090/1‑149 (MQ=255)
                                                                            gcgcCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCg                                                                 <  5:275481/149‑1 (MQ=255)
                                                                            gcgcCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCg                                                                 <  4:13670/149‑1 (MQ=255)
                                                                            gcgcCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCg                                                                 <  8:42090/149‑1 (MQ=255)
                                                                              gcCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGc                                                               <  7:129230/149‑1 (MQ=255)
                                                                               ccTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCa                                                              >  6:231722/1‑149 (MQ=255)
                                                                               ccTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCa                                                              <  4:17346/149‑1 (MQ=255)
                                                                                ctgctgGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCAt                                                             >  8:174563/1‑149 (MQ=255)
                                                                                     ggACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTcc                                                        >  7:83192/1‑149 (MQ=255)
                                                                                      gACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTTGCCCTGCAGTCCCGCCCACCCAAGGTGCGGGtt                                                                                                      <  7:47251/102‑1 (MQ=255)
                                                                                      gACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGtt                                                                                                      >  8:47251/1‑102 (MQ=255)
                                                                                        cAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACg                                                     <  5:56056/149‑1 (MQ=255)
                                                                                           tGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGcc                                                  >  4:122313/1‑149 (MQ=255)
                                                                                            ggCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCct                                                 >  5:133951/1‑149 (MQ=255)
                                                                                                      acGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGcc                                       <  4:146528/149‑1 (MQ=255)
                                                                                                      acGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGcc                                       >  5:130414/1‑149 (MQ=255)
                                                                                                         tggtggtgTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAg                                    <  3:122313/149‑1 (MQ=255)
                                                                                                         tggtggtgTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATCGTCCACGGCCTGCTGCTGGCCGAg                                    <  1:124284/149‑1 (MQ=255)
                                                                                                             ggtgTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGgcgc                                <  6:210814/149‑1 (MQ=255)
                                                                                                                 tCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTgc                            <  7:174563/149‑1 (MQ=255)
                                                                                                                 tCTGCGGGCGAGCAGGGCGTCAGTTGGCTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCTGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTgc                            <  8:57930/149‑1 (MQ=255)
                                                                                                                 tCTGCGGGCGAGCAGGGCGTCAGTTGGCTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTgc                            <  8:57920/149‑1 (MQ=255)
                                                                                                                   tGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTgcg                           >  8:67317/1‑148 (MQ=255)
                                                                                                                   tGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTgcg                           >  5:13337/1‑148 (MQ=255)
                                                                                                                     cGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTgcg                           <  6:13337/146‑1 (MQ=255)
                                                                                                                      gggCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGa                       >  5:170492/1‑149 (MQ=255)
                                                                                                                            gCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCACTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGa                 >  4:159067/1‑149 (MQ=255)
                                                                                                                             cAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCACCTGCGCAGACGTTGAc                >  5:164563/1‑149 (MQ=255)
                                                                                                                              aGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGAcc               <  5:231722/149‑1 (MQ=255)
                                                                                                                              aGGGCGTCAGGGGGGGCAGGCGCGAGCGGGCCCTGCAGGCCCGCCCCCCCAAGGGGCGGGTTGCCAGCACGGTGGGGGCGGGGGCGGCGCGGGTGGCCGGCATGGTCCACGGCCGGCTGCTGGCCGCGGCGCCTGCGCCGGCGtggccc               >  4:192653/1‑144 (MQ=255)
                                                                                                                                 gCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGACCCgg            <  6:170492/149‑1 (MQ=255)
                                                                                                                                       gTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGACCCGGGCCACt      >  2:17087/1‑149 (MQ=255)
                                                                                                                                       gTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACCGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGACCCGGGCCACt      >  6:73757/1‑149 (MQ=255)
                                                                                                                                           gTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGACCCGGGCCACTGCCa  >  7:60640/1‑149 (MQ=255)
                                                                                                                                           gTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGACCCGGGCCACTGCCa  <  6:133951/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
AGCCCAATACCGAAGAACTGGGCGAAGTGCTGGGCCTGGCTGTGGATAACCTGACGCAACAGCGCGCTGCCGCCAAGCGCCTGCTGGACAGTGGCGTTGAACACGTGGTGGTGTCTGCGGGCGAGCAGGGCGTCAGTTGGTTCAGTCGCGATCTGGCCCTGCAGGCCCGCCCACCCAAGGTGCGGGTTGCCAGCACGGTGGGGGCGGGGGATTCGCTGGTGGCCGGCATGGTCCACGGCCTGCTGCTGGCCGAGGCGCCTGCGCAGACGTTGACCCGGGCCACTGCCA  >  NC_002947/912896‑913183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑