Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 913,888 G→A 9.7% G200D (GGC→GAC)  fruA → PTS fructose transporter subunit IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947913,8880GA 9.7% 106.5 / 2.2 41G200D (GGC→GAC) fruAPTS fructose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base G (17/20);  new base A (2/2);  total (19/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGACCGTCGAGACCCAGGGCTCGGTCGGTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTACAAGCACTTGCTGACCGGCGTCTCG  >  NC_002947/913740‑914033
                                                                                                                                                    |                                                                                                                                                 
ttGACCGTCGAGACCCAGGGCTCGGTCGGTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTgg                                                                                                                                                   >  6:84210/1‑149 (MQ=255)
       tCGAGACCCAGGGCTCGGTCGGTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGcc                                                                                                                                            <  6:159910/149‑1 (MQ=255)
              ccAGGGCTCGGTCGGTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAg                                                                                                                                      >  5:19757/1‑148 (MQ=255)
              ccAGGGCTCGGTCGGTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAg                                                                                                                                      >  1:72937/1‑148 (MQ=255)
               cAGGGCTCGGTCGGTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGct                                                                                                                                    <  7:221456/149‑2 (MQ=255)
                     tcggtcggTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCcg                                                                                                                              >  1:10921/1‑149 (MQ=255)
                       ggtcggtGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCcgc                                                                                                                             <  2:72937/148‑1 (MQ=255)
                             agcgcgcAATCCCTTGTCTGCTGAAGCCATCGCAGAGTCGGACGTGCTGCTGCTCGCCGCCAACATTGAAATACACACAGCACCTTTCGCCAGCAAACGTATTTACCCTTGAGGTACTGGCATTGACCTCAAGCAGGCCCGCGCTAccc                                                                                                                      >  6:95872/2‑149 (MQ=255)
                               cgcgcAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCt                                                                                                                     <  6:289755/148‑1 (MQ=255)
                                      tCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAg                                                                                                             >  5:258206/1‑149 (MQ=255)
                                         cTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGccc                                                                                                          >  4:71831/1‑149 (MQ=255)
                                            gTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTg                                                                                                        <  6:19757/148‑1 (MQ=255)
                                            gTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTg                                                                                                        <  3:12244/148‑1 (MQ=255)
                                            gTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTg                                                                                                        <  3:288881/148‑1 (MQ=255)
                                              ctgctgTAGCCATCGATTCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGACTTACCCACTTCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGgc                                                                                                      <  8:274109/148‑1 (MQ=255)
                                                  tgAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAgg                                                                                                 <  3:71831/149‑1 (MQ=255)
                                                                   cggACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGt                                                                                <  8:147623/149‑1 (MQ=255)
                                                                              ctgctgGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGc                                                                     <  5:84210/149‑1 (MQ=255)
                                                                                gctgGCCGCCGCCATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTAGCGTTGTGGTACTGGCATTGCCCTCAAGCAGGACCGCGCTACCCTGGACACAGCCCTGGCGCAGGCCACGGTGGAACACTGTGCCGACGCTGccg                                                                   >  5:151291/1‑147 (MQ=255)
                                                                                      cgccgACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAATCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGa                                                             <  6:258206/149‑1 (MQ=255)
                                                                                             aTTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGcc                                                      >  2:207111/1‑149 (MQ=255)
                                                                                              ttGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGcc                                                      >  3:50924/1‑148 (MQ=255)
                                                                                                gAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGAc                                                   >  1:219843/1‑149 (MQ=255)
                                                                                                   gTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCaa                                                >  4:303109/1‑149 (MQ=255)
                                                                                                      cccACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTgg                                                                                        >  4:148428/1‑106 (MQ=255)
                                                                                                      cccACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTgg                                                                                        <  3:148428/106‑1 (MQ=255)
                                                                                                        cACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCg                                           <  5:89303/149‑1 (MQ=255)
                                                                                                         aCTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCga                                          <  2:219843/149‑1 (MQ=255)
                                                                                                         aCTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCga                                          <  2:10921/149‑1 (MQ=255)
                                                                                                             cACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGTCAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGaa                                       >  6:311361/1‑148 (MQ=255)
                                                                                                               cGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAAc                                    <  2:73891/149‑1 (MQ=255)
                                                                                                                gTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAAcc                                   <  1:207111/149‑1 (MQ=255)
                                                                                                                         gCAAACGTATTTACCGTTGTGGTACTGACATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTa                           >  6:199864/1‑148 (MQ=255)
                                                                                                                         gCAAACGTATTTACCGTTGTGGTACTGACATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTa                           >  6:195425/1‑148 (MQ=255)
                                                                                                                           aaaCGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTAc                          >  3:55207/1‑147 (MQ=255)
                                                                                                                                    tACCGTTGTGGTACTGACATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTACAAGCACTTGc                <  5:199864/148‑1 (MQ=255)
                                                                                                                                    tACCGTTGTGGTACTGACATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTACAAGCACTTGc                <  5:195425/148‑1 (MQ=255)
                                                                                                                                      ccGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTACAAGCACTTGCTGa             <  4:50924/149‑1 (MQ=255)
                                                                                                                                       cGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTACAAGCACTTGCTGAc            >  4:288816/1‑149 (MQ=255)
                                                                                                                                                aCTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGc                                                       <  5:70391/97‑1 (MQ=255)
                                                                                                                                                aCTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGc                                                       >  6:70391/1‑97 (MQ=255)
                                                                                                                                                 cTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTACAAGCACTTGCTGACCGGCGTCTCg  >  8:1253/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTGACCGTCGAGACCCAGGGCTCGGTCGGTGCGCGCAATCCCTTGTCTGCTGAAGCCATCGCAGCGGCGGACGTGGTGCTGCTGGCCGCCGACATTGAAGTACCCACTGCACGTTTCGCCGGCAAACGTATTTACCGTTGTGGTACTGGCATTGCCCTCAAGCAGGCCCGCGCTACCCTGGACAAAGCCCTGGCGCAGGCCACGGTGGAAAACGGTGCCGACGCTGCAGCGGCGACCACGCCGACCAAGAGCGAGAAAACCGGCGTGTACAAGCACTTGCTGACCGGCGTCTCG  >  NC_002947/913740‑914033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑