Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 914,163 A→T 8.5% K292* (AAA→TAA)  fruA → PTS fructose transporter subunit IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947914,1630AT 8.5% 135.6 / 3.0 47K292* (AAA→TAA) fruAPTS fructose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (20/23);  new base T (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TTGCTGACCGGCGTCTCGTTCATGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCATTGTCGCCGGTTTCCTCGCCG  >  NC_002947/914016‑914307
                                                                                                                                                   |                                                                                                                                                
ttGCTGACCGGCGTCTCGTTCATGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGCTTGGCGGTGAGGCTGCCTTCa                                                                                                                                                  >  8:94324/1‑148 (MQ=255)
   cTGACCGGCGTCTCGTTCATGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACt                                                                                                                                              >  4:80338/1‑149 (MQ=255)
       ccGGCGTGTGGTTCATGCTGCCGATGGGGGTGGCCGGCGGCCTGGGGATTGCGTTGTCGTTCGGGTTCGCTAGCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGGGGCGTTGATGCAGATTGGCGGTGAGGCGGCCTTCAAACTGATg                                                                                                                                          <  6:151291/149‑1 (MQ=255)
             tctcGTTCATGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCg                                                                                                                                    <  3:272740/149‑1 (MQ=255)
                 gTTCATGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTAc                                                                                                                                >  3:206033/1‑149 (MQ=255)
                    cATGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTg                                                                                                                              <  3:54904/148‑1 (MQ=255)
                     aTGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTgg                                                                                                                             <  5:9200/148‑1 (MQ=255)
                     aTGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGGATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGATCCGCTACTGGc                                                                                                                            <  4:86732/149‑1 (MQ=255)
                            cGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCta                                                                                                                      >  5:181702/1‑148 (MQ=255)
                             gATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCtata                                                                                                                    >  5:225727/1‑149 (MQ=255)
                                  tggtggCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGcc                                                                                                               <  6:199277/149‑1 (MQ=255)
                                    gtggCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCt                                                                                                              <  6:289993/148‑1 (MQ=255)
                                    gtggCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTg                                                                                                             >  2:255687/1‑149 (MQ=255)
                                      ggCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGt                                                                                                           <  4:206033/149‑1 (MQ=255)
                                         cggcggCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTa                                                                                                        >  5:128825/1‑149 (MQ=255)
                                                ctgctgATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGa                                                                                                 >  6:230391/1‑149 (MQ=255)
                                                   ctgATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCTAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCg                                                                                              >  6:197462/1‑149 (MQ=255)
                                                   ctgATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCTAACTGATGGTTCCGCTACAGGCAGGCTATATCGCCTGGTCTATCGCCGACCg                                                                                              >  6:201887/1‑149 (MQ=255)
                                                           gTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGc                                                                                      <  6:16918/149‑1 (MQ=255)
                                                            ttGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCt                                                                                     <  2:135237/149‑1 (MQ=255)
                                                              gTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTg                                                                                    <  1:255687/148‑1 (MQ=255)
                                                                 gttcgtGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGccc                                                                                >  2:305559/1‑149 (MQ=255)
                                                                     gtgtTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCgg                                                                             <  6:110713/148‑1 (MQ=255)
                                                                       gtTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCgggaa                                                                          >  6:256292/1‑147 (MQ=255)
                                                                          cGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGcc                                                                                                   >  6:312818/1‑121 (MQ=255)
                                                                          cGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGcc                                                                                                   <  5:312818/121‑1 (MQ=255)
                                                                           ggTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGa                                                                       <  6:181702/148‑1 (MQ=255)
                                                                              aTCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGc                                                                           <  4:225745/141‑1 (MQ=255)
                                                                              aTCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGc                                                                           >  3:225745/1‑141 (MQ=255)
                                                                                 gAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATcggcgg                                                                <  2:252744/149‑1 (MQ=255)
                                                                                    gCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCt                                                             >  5:172778/1‑149 (MQ=255)
                                                                                                gCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCa                                                  >  8:121111/1‑148 (MQ=255)
                                                                                                      aCCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTggg                                           >  3:223074/1‑149 (MQ=255)
                                                                                                      aCCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTggg                                           <  5:230391/149‑1 (MQ=255)
                                                                                                      aCCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTggg                                           >  7:28591/1‑149 (MQ=255)
                                                                                                      aCCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTggg                                           >  8:225542/1‑149 (MQ=255)
                                                                                                       ccTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTgggg                                          <  7:225542/149‑1 (MQ=255)
                                                                                                           gCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGcc                                       <  8:234396/148‑1 (MQ=255)
                                                                                                                cggcgTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCTGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTc                                 <  4:223074/149‑1 (MQ=255)
                                                                                                                       gATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGt                           <  7:94324/148‑1 (MQ=255)
                                                                                                                        aTGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCGGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTg                          <  6:128825/148‑1 (MQ=255)
                                                                                                                         tGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTgg                         >  1:15831/1‑148 (MQ=255)
                                                                                                                           cAGATTGGCGGTGAGGCTGCCTTCTAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCTGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCa                       <  5:197462/148‑1 (MQ=255)
                                                                                                                           cAGATTGGCGGTGAGGCTGCCTTCTAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCa                       <  5:201887/148‑1 (MQ=255)
                                                                                                                                tGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCATTGTCg                 >  8:50041/1‑149 (MQ=255)
                                                                                                                                           cTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCATTGTCGCCGGTTTCCTc      >  2:285575/1‑149 (MQ=255)
                                                                                                                                              ccTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCGCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCATTGTCGCCGGTTTCCTCGcc   <  7:121111/149‑1 (MQ=255)
                                                                                                                                               cTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCATTGTCGCCGGTTTCCTCGCCg  >  7:197732/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTGCTGACCGGCGTCTCGTTCATGCTTCCGATGGTGGTGGCCGGCGGCCTGCTGATTGCGTTGTCGTTCGTGTTCGGTATCGAGGCCTACAAAGAGGCGGGCACCTTGCCGGCGGCGTTGATGCAGATTGGCGGTGAGGCTGCCTTCAAACTGATGGTTCCGCTACTGGCAGGCTATATCGCCTGGTCTATCGCCGACCGCCCCGGGCTGGCCCCGGGCATGATCGGCGGCCTGTTGGCCAGCACCTTGGGGGCCGGCTTCATCGGTGGCATTGTCGCCGGTTTCCTCGCCG  >  NC_002947/914016‑914307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑