Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 931,502 A→T 6.0% V4707D (GTC→GAC)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947931,5020AT 6.0% 201.6 / 2.2 66V4707D (GTC→GAC) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (32/30);  new base T (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGG  >  NC_002947/931357‑931648
                                                                                                                                                 |                                                                                                                                                  
gCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGacac                                                                                                                                                 >  7:159847/1‑149 (MQ=255)
      cagcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAc                                                                                                                                           <  5:191068/149‑1 (MQ=255)
        gcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGATGGTGTCACCCTCACCg                                                                                                                                         <  5:42401/149‑1 (MQ=255)
         cTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCg                                                                                                                                         >  7:190460/1‑148 (MQ=255)
          tCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCgg                                                                                                                                        >  2:255017/1‑148 (MQ=255)
                ttGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGc                                                                                                                                 <  8:181997/149‑1 (MQ=255)
                  gCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGc                                                                                                                                 >  3:54716/1‑147 (MQ=255)
                    ccAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCCGTGAGGGTGACACCATCACCGGAAACGGc                                                                                                                                 >  2:268339/1‑145 (MQ=255)
                          ccGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCg                                                                                                                       >  1:152553/1‑149 (MQ=255)
                             ggCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAcc                                                                                                                                          >  2:39834/1‑127 (MQ=255)
                             ggCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAcc                                                                                                                                          <  1:39834/127‑1 (MQ=255)
                              gCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTg                                                                                                                   >  2:60560/1‑149 (MQ=255)
                                tCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGcc                                                                                                                 >  3:217370/1‑149 (MQ=255)
                                   gCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGAt                                                                                                              <  1:255017/149‑1 (MQ=255)
                                        aTGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCgg                                                                                                         >  4:66730/1‑149 (MQ=255)
                                        aTGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCg                                                                                                          <  5:214169/148‑1 (MQ=255)
                                         tGGTGAAGTGGCCACTGGCATCGATGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgt                                                                                                        <  5:139258/149‑1 (MQ=255)
                                          ggTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtg                                                                                                       >  2:12164/1‑149 (MQ=255)
                                           gTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                      >  1:55341/1‑149 (MQ=255)
                                            tGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGtgtg                                                                                                     <  2:20676/149‑1 (MQ=255)
                                            tGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGtgtg                                                                                                     <  8:190460/149‑1 (MQ=255)
                                                gTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACAccggccg                                                                                                                                           >  7:81005/1‑102 (MQ=255)
                                                gTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACAccggccg                                                                                                                                           <  8:81005/107‑6 (MQ=255)
                                                   gCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGATGGTGTCACCCTCACCg                                                                                                                                         <  5:144611/106‑1 (MQ=255)
                                                   gCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGATGGTGTCACCCTCACCg                                                                                                                                         >  6:144611/1‑106 (MQ=255)
                                                    ccACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                      <  7:230610/140‑1 (MQ=255)
                                                    ccACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                      >  8:230610/1‑140 (MQ=255)
                                                    ccACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCggg                                                                                             >  1:178177/1‑149 (MQ=255)
                                                        tGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCg                                                                                         >  2:112069/1‑149 (MQ=255)
                                                             tCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGa                                                                                     >  4:161255/1‑148 (MQ=255)
                                                                  ggTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAgcgc                                                                               <  2:152553/149‑1 (MQ=255)
                                                                    tGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTg                                                                             >  1:293461/1‑149 (MQ=255)
                                                                    tGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTg                                                                             >  3:290383/1‑149 (MQ=255)
                                                                    tGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTg                                                                             <  8:7802/149‑1 (MQ=255)
                                                                        gcTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTggcgg                                                                         >  2:262275/1‑149 (MQ=255)
                                                                          tGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAgcgc                                                                               >  7:90083/1‑141 (MQ=255)
                                                                          tGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAgcgc                                                                               <  8:90083/141‑1 (MQ=255)
                                                                                    cTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgc                                                             >  1:60886/1‑149 (MQ=255)
                                                                                    cTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtg                                                              <  3:274338/148‑1 (MQ=255)
                                                                                       gCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTCATAGCGCTGGCGGCAGACTGGTtgcctg                                                          <  1:60560/149‑1 (MQ=255)
                                                                                        ccTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcctgc                                                         <  4:54716/149‑1 (MQ=255)
                                                                                        ccTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcctgc                                                         <  8:159847/149‑1 (MQ=255)
                                                                                         cTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTgg                                                                 >  1:242704/1‑140 (MQ=255)
                                                                                         cTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTgg                                                                 <  2:242704/140‑1 (MQ=255)
                                                                                         cTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcctgcc                                                        <  1:112069/149‑1 (MQ=255)
                                                                                                gTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTggcg                                                                          >  5:102779/1‑124 (MQ=255)
                                                                                                gTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTggcg                                                                          <  6:102779/124‑1 (MQ=255)
                                                                                                 ttGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGc                                                <  1:149454/149‑1 (MQ=255)
                                                                                                       aCGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGcac                                          >  2:111515/1‑149 (MQ=255)
                                                                                                        cGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGcac                                          >  6:83592/1‑148 (MQ=255)
                                                                                                                              gccgcGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttgg                   <  2:55341/149‑1 (MQ=255)
                                                                                                                              gccgcGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttg                    <  1:262275/148‑1 (MQ=255)
                                                                                                                               ccgcGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttggc                  <  4:290383/149‑1 (MQ=255)
                                                                                                                                 gcgGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCt                >  4:185807/1‑149 (MQ=255)
                                                                                                                                   ttccGGTGATGGTGTCACCCTCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCt                >  1:121750/3‑147 (MQ=255)
                                                                                                                                    gCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgt                             >  6:238785/1‑133 (MQ=255)
                                                                                                                                    gCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgt                             <  5:238785/133‑1 (MQ=255)
                                                                                                                                       ggTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGt                                                                <  2:15334/95‑1 (MQ=255)
                                                                                                                                       ggTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGt                                                                >  1:15334/1‑95 (MQ=255)
                                                                                                                                       ggTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGc           >  3:270461/1‑148 (MQ=255)
                                                                                                                                           aGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGc                                   <  3:219636/120‑1 (MQ=255)
                                                                                                                                           aGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGc                                   >  4:219636/1‑120 (MQ=255)
                                                                                                                                             ggTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATAc      >  7:10418/1‑147 (MQ=255)
                                                                                                                                             ggTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGATGCCTGCCGCATCGGCCAGCCCCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATAc      >  1:280323/1‑147 (MQ=255)
                                                                                                                                               tGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAgg  <  2:60886/149‑1 (MQ=255)
                                                                                                                                               tGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAgg  <  3:185807/149‑1 (MQ=255)
                                                                                                                                               tGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTAGCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAgg  <  3:66730/149‑1 (MQ=255)
                                                                                                                                                 acacCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttggcc                 >  3:15331/1‑132 (MQ=255)
                                                                                                                                                 acacCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttggcc                 <  4:15331/132‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCTGAGCAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGG  >  NC_002947/931357‑931648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑