Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 931,507 A→C 6.0% D4705E (GAT→GAG)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947931,5070AC 6.0% 202.7 / 3.0 67D4705E (GAT→GAG) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base A (33/30);  new base C (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGA  >  NC_002947/931363‑931652
                                                                                                                                                |                                                                                                                                                 
cagcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAc                                                                                                                                               <  5:191068/149‑1 (MQ=255)
  gcTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGATGGTGTCACCCTCACCg                                                                                                                                             <  5:42401/149‑1 (MQ=255)
   cTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCg                                                                                                                                             >  7:190460/1‑148 (MQ=255)
    tCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCgg                                                                                                                                            >  2:255017/1‑148 (MQ=255)
          ttGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGc                                                                                                                                     <  8:181997/149‑1 (MQ=255)
            gCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGc                                                                                                                                     >  3:54716/1‑147 (MQ=255)
              ccAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCCGTGAGGGTGACACCATCACCGGAAACGGc                                                                                                                                     >  2:268339/1‑145 (MQ=255)
                    ccGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCg                                                                                                                           >  1:152553/1‑149 (MQ=255)
                       ggCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAcc                                                                                                                                              <  1:39834/127‑1 (MQ=255)
                       ggCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCAcc                                                                                                                                              >  2:39834/1‑127 (MQ=255)
                        gCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTg                                                                                                                       >  2:60560/1‑149 (MQ=255)
                          tCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGcc                                                                                                                     >  3:217370/1‑149 (MQ=255)
                             gCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGAt                                                                                                                  <  1:255017/149‑1 (MQ=255)
                                  aTGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCgg                                                                                                             >  4:66730/1‑149 (MQ=255)
                                  aTGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCg                                                                                                              <  5:214169/148‑1 (MQ=255)
                                   tGGTGAAGTGGCCACTGGCATCGATGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgt                                                                                                            <  5:139258/149‑1 (MQ=255)
                                    ggTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtg                                                                                                           >  2:12164/1‑149 (MQ=255)
                                     gTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                          >  1:55341/1‑149 (MQ=255)
                                      tGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGtgtg                                                                                                         <  8:190460/149‑1 (MQ=255)
                                      tGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGtgtg                                                                                                         <  2:20676/149‑1 (MQ=255)
                                             gCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGATGGTGTCACCCTCACCg                                                                                                                                             >  6:144611/1‑106 (MQ=255)
                                             gCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGATGGTGTCACCCTCACCg                                                                                                                                             <  5:144611/106‑1 (MQ=255)
                                              ccACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                          >  8:230610/1‑140 (MQ=255)
                                              ccACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGgtgt                                                                                                          <  7:230610/140‑1 (MQ=255)
                                              ccACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCggg                                                                                                 >  1:178177/1‑149 (MQ=255)
                                                  tGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCg                                                                                             >  2:112069/1‑149 (MQ=255)
                                                       tCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGa                                                                                         >  4:161255/1‑148 (MQ=255)
                                                            ggTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAgcgc                                                                                   <  2:152553/149‑1 (MQ=255)
                                                              tGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTg                                                                                 <  8:7802/149‑1 (MQ=255)
                                                              tGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTg                                                                                 >  3:290383/1‑149 (MQ=255)
                                                              tGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTg                                                                                 >  1:293461/1‑149 (MQ=255)
                                                                  gcTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTggcgg                                                                             >  2:262275/1‑149 (MQ=255)
                                                                    tGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAgcgc                                                                                   >  7:90083/1‑141 (MQ=255)
                                                                    tGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAgcgc                                                                                   <  8:90083/141‑1 (MQ=255)
                                                                              cTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgc                                                                 >  1:60886/1‑149 (MQ=255)
                                                                              cTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtg                                                                  <  3:274338/148‑1 (MQ=255)
                                                                                 gCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTCATAGCGCTGGCGGCAGACTGGTtgcctg                                                              <  1:60560/149‑1 (MQ=255)
                                                                                  ccTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcctgc                                                             <  8:159847/149‑1 (MQ=255)
                                                                                  ccTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcctgc                                                             <  4:54716/149‑1 (MQ=255)
                                                                                   cTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTgg                                                                     <  2:242704/140‑1 (MQ=255)
                                                                                   cTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTgg                                                                     >  1:242704/1‑140 (MQ=255)
                                                                                   cTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTtgcctgcc                                                            <  1:112069/149‑1 (MQ=255)
                                                                                          gTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTggcg                                                                              >  5:102779/1‑124 (MQ=255)
                                                                                          gTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTggcg                                                                              <  6:102779/124‑1 (MQ=255)
                                                                                           ttGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGc                                                    <  1:149454/149‑1 (MQ=255)
                                                                                                 aCGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGcac                                              >  2:111515/1‑149 (MQ=255)
                                                                                                  cGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGcac                                              >  6:83592/1‑148 (MQ=255)
                                                                                                                        gccgcGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttgg                       <  2:55341/149‑1 (MQ=255)
                                                                                                                        gccgcGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttg                        <  1:262275/148‑1 (MQ=255)
                                                                                                                         ccgcGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttggc                      <  4:290383/149‑1 (MQ=255)
                                                                                                                           gcgGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCt                    >  4:185807/1‑149 (MQ=255)
                                                                                                                             ttccGGTGATGGTGTCACCCTCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCt                    >  1:121750/3‑147 (MQ=255)
                                                                                                                              gCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgt                                 <  5:238785/133‑1 (MQ=255)
                                                                                                                              gCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgt                                 >  6:238785/1‑133 (MQ=255)
                                                                                                                                 ggTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGt                                                                    <  2:15334/95‑1 (MQ=255)
                                                                                                                                 ggTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGt                                                                    >  1:15334/1‑95 (MQ=255)
                                                                                                                                 ggTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGc               >  3:270461/1‑148 (MQ=255)
                                                                                                                                     aGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGc                                       >  4:219636/1‑120 (MQ=255)
                                                                                                                                     aGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGc                                       <  3:219636/120‑1 (MQ=255)
                                                                                                                                       ggTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATAc          >  7:10418/1‑147 (MQ=255)
                                                                                                                                       ggTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGATGCCTGCCGCATCGGCCAGCCCCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATAc          >  1:280323/1‑147 (MQ=255)
                                                                                                                                         tGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAgg      <  3:185807/149‑1 (MQ=255)
                                                                                                                                         tGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAgg      <  2:60886/149‑1 (MQ=255)
                                                                                                                                         tGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTAGCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAgg      <  3:66730/149‑1 (MQ=255)
                                                                                                                                           acacCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttggcc                     <  4:15331/132‑1 (MQ=255)
                                                                                                                                           acacCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTgttggccgttggcc                     >  3:15331/1‑132 (MQ=255)
                                                                                                                                             acCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGa  >  5:274518/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CAGCTCGGCGTTGCCCAGTGCCGGGCTCAGCGTGATGGTGAAGTGGCCACTGGCATCGACGGTGGCGCTGCCGATTTCCTCGCCTGCCGGGTTGGTCACGGTAACCCGTGCGCCCACTTCGCCGCGGCCGGTGAGGGTGACACCATCACCGGAAACGGCCAGTTCGCCGGGTGCCGATGGCGGTGTGGTATCGGGTGCGGTGATAGCGCTGGCGGCAGACTGGTTGCCTGCCGCATCGGCCAGCACCACTTGCACCTGTTGGCCGTTGGCCTGAGGCGATACGAGGTTGA  >  NC_002947/931363‑931652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑