Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 938,626 C→T 8.5% V2332V (GTG→GTA)  PP_0806 ← surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947938,6260CT 8.5% 132.7 / 2.1 47V2332V (GTG→GTA) PP_0806surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (21/22);  new base T (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

CGGCCGTGGAGGTGTTGCCGGCGGCGTCGGTGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGCCATCGATGGC  >  NC_002947/938481‑938772
                                                                                                                                                  |                                                                                                                                                  
cggccgTGGAGGTGTTGCCGGCGGCGTCGGTGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGcaca                                                                                                                                                 >  1:136782/1‑149 (MQ=255)
      tGGAGGCGTTGCCGGCGGCGTCGGTGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCAcacgcacg                                                                                                                                           <  5:228144/149‑1 (MQ=255)
                gCCGGCGGCGTCGGTGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCa                                                                                                                                 >  8:179850/1‑149 (MQ=255)
                   ggcggcGTCGGTGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACtt                                                                                                                              >  3:63984/1‑149 (MQ=255)
                           cGGTGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCgg                                                                                                                      <  7:179850/149‑1 (MQ=255)
                           cGGGGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGGCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCgg                                                                                                                      <  8:59607/149‑1 (MQ=255)
                              tGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCg                                                                                                                   <  6:2185/149‑1 (MQ=255)
                                gCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGt                                                                                                                 >  3:20056/1‑149 (MQ=255)
                                 cGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGCCCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTg                                                                                                                >  3:188626/1‑149 (MQ=255)
                                 cGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCGCGCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGt                                                                                                                 <  1:62658/149‑1 (MQ=255)
                                 cGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTg                                                                                                                <  3:150896/149‑1 (MQ=255)
                                        ttGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGc                                                                                                         >  6:159114/1‑149 (MQ=255)
                                        ttGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACGTCGGCGCGGCCGGTGACCTCGc                                                                                                         >  7:122825/1‑149 (MQ=255)
                                                ccTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGTACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTg                                                                                                 >  2:289136/1‑149 (MQ=255)
                                                ccTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGTACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTg                                                                                                 >  2:293718/1‑149 (MQ=255)
                                                 ctctCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTgg                                                                                                >  5:232126/1‑149 (MQ=255)
                                                  tctcCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTgg                                                                                                >  4:92377/1‑148 (MQ=255)
                                                      cACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCa                                                                                              <  6:135112/146‑1 (MQ=255)
                                                      cACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCa                                                                                              <  4:63984/146‑1 (MQ=255)
                                                      cACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTg                                                                                           <  5:159114/149‑1 (MQ=255)
                                                             cTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGTACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTa                                                                                    <  1:289136/149‑1 (MQ=255)
                                                             cTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGTACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTa                                                                                    <  1:293718/149‑1 (MQ=255)
                                                                cggcggGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCg                                                                                       <  7:146740/143‑1 (MQ=255)
                                                                cggcggGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCg                                                                                       >  8:146740/1‑143 (MQ=255)
                                                                  gcggGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAgctcg                                                                               >  3:277973/1‑149 (MQ=255)
                                                                  gcggGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAgctcg                                                                               >  6:17321/1‑149 (MQ=255)
                                                                        cGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCggg                                                                         <  7:235938/149‑1 (MQ=255)
                                                                                  gCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACcggcg                                                               >  3:95942/1‑149 (MQ=255)
                                                                                  gCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACcggcg                                                               >  6:176606/1‑149 (MQ=255)
                                                                                      aaaCTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCgggg                                                            <  4:20056/148‑1 (MQ=255)
                                                                                      aaaCTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCggg                                                             <  4:277973/147‑1 (MQ=255)
                                                                                      aaaCTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGAACGCTAGCTCGCTCGGGGTGACCGGCgggg                                                            <  1:111148/148‑1 (MQ=255)
                                                                                           gCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAACACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGt                                                      >  1:199937/1‑149 (MQ=255)
                                                                                              aGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTg                                                   <  5:176606/149‑1 (MQ=255)
                                                                                               gTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTgg                                                  <  3:92377/149‑1 (MQ=255)
                                                                                                  gCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACCCGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGATGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGCGTGATGTCTGgcg                                                >  3:234691/1‑148 (MQ=255)
                                                                                                        accaccACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGAt                                         <  2:103625/149‑1 (MQ=255)
                                                                                                           accacACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTg                                      <  5:17321/149‑1 (MQ=255)
                                                                                                                     ccAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCt                                                                                     >  8:214919/1‑92 (MQ=255)
                                                                                                                      cAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAgg                            >  2:283811/1‑148 (MQ=255)
                                                                                                                                  gCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCgg               <  4:188626/149‑1 (MQ=255)
                                                                                                                                   ccATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGt              <  6:232126/149‑1 (MQ=255)
                                                                                                                                     aTCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGc            >  5:29995/1‑149 (MQ=255)
                                                                                                                                              ccAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGCCATCGATg    <  7:171631/149‑1 (MQ=255)
                                                                                                                                               cAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGCCATCGATgg   >  3:79726/1‑149 (MQ=255)
                                                                                                                                                 gCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGCCATCGAt     <  1:45937/145‑1 (MQ=255)
                                                                                                                                                 gCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGCCATCGAt     >  2:45937/1‑145 (MQ=255)
                                                                                                                                                 gCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTAGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGCCATCGATGGc  >  5:227774/1‑148 (MQ=255)
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CGGCCGTGGAGGTGTTGCCGGCGGCGTCGGTGGCGGTGACTTGTAGTACCTCTCCACTCACCTGCGGCGGGTCGAGGGTGATGCTGAAACTGCCAGTGGCGTCGACCACCACACTACCCAGCACGGTGGTGCCATCG‑GCGGCCAGCACACGCACGGTGCTGCCCACTTCGGCGCGGCCGGTGACCTCGCTGCCGTTGGCACTGATCGCTAGCTCGCTCGGGGTGACCGGCGGGGTGATGTCTGGCGCAGCGATTTGCACCGGCAGGGACGAAGCGCCGGTGCCATCGATGGC  >  NC_002947/938481‑938772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑