Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,001,196 A→T 6.4% Q58L (CAG→CTG)  PP_0863 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,001,1960AT 6.4% 233.6 / 2.9 77Q58L (CAG→CTG) PP_0863hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (42/30);  new base T (3/2);  total (45/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCG  >  NC_002947/1001054‑1001337
                                                                                                                                              |                                                                                                                                             
gtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGCTTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCCGCAGGATGc                                                                                                                                         >  7:171029/1‑149 (MQ=255)
   gATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCTGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCAc                                                                                                                                      <  1:283776/149‑1 (MQ=255)
   gATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCAc                                                                                                                                      >  4:289843/1‑149 (MQ=255)
   gATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCAc                                                                                                                                      >  2:191535/1‑149 (MQ=255)
    aTGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACg                                                                                                                                     >  7:74305/1‑149 (MQ=255)
               ttGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCg                                                                                                                          >  7:89460/1‑149 (MQ=255)
                         tGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGt                                                                                                                <  3:255949/149‑1 (MQ=255)
                          gCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTa                                                                                                               >  7:121692/1‑149 (MQ=255)
                            tCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGt                                                                                                                >  5:259370/1‑146 (MQ=255)
                            tCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGt                                                                                                                <  6:259370/146‑1 (MQ=255)
                              gAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGc                                                                                                           >  4:101004/1‑149 (MQ=255)
                               aGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                                          >  2:150556/1‑149 (MQ=255)
                               aGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCt                                                                                                          <  7:272397/149‑1 (MQ=255)
                                ggAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTg                                                                                                         <  8:104796/149‑1 (MQ=255)
                                ggAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCAGTATCGCTg                                                                                                         >  6:221102/1‑149 (MQ=255)
                                      tCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAgg                                                                                                   >  7:94084/1‑149 (MQ=255)
                                         cGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTg                                                                                                <  3:289843/149‑1 (MQ=255)
                                          ggCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAg                                                                                                    >  1:135039/1‑144 (MQ=255)
                                          ggCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAg                                                                                                    <  2:135039/144‑1 (MQ=255)
                                              aGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGt                                                                                           <  4:157345/149‑1 (MQ=255)
                                                gccgccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGc                                                                                         >  7:203414/1‑149 (MQ=255)
                                                   gccCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCAt                                                                                      <  8:188414/149‑1 (MQ=255)
                                                    cccAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATg                                                                                     <  8:74305/149‑1 (MQ=255)
                                                      cAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGGGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGgc                                                                                   <  1:191535/149‑1 (MQ=255)
                                                         gCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGgcgc                                                                                 <  6:13995/148‑1 (MQ=255)
                                                         gCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGgcgc                                                                                 >  5:13995/1‑148 (MQ=255)
                                                                  gcggcAGGTGGGCAGGGTGTGGTAGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCTTCGTTGGTTCGGTATAGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGc                                                                        <  8:203414/148‑1 (MQ=255)
                                                                    ggcAGCTGGGCAGGGTGGGGTCGAGGCGCAGGTGCTGCTGGGGCAGATCCTGCGGGATGGGCGGGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCg                                                                     <  8:171029/149‑1 (MQ=255)
                                                                      cAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCggg                                                                   >  7:109106/1‑149 (MQ=255)
                                                                         cGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCTTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGTGCGt                                                                <  8:89460/149‑1 (MQ=255)
                                                                          ggggCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGtt                                                               >  8:37343/1‑149 (MQ=255)
                                                                           gggCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTg                                                              >  4:292611/1‑149 (MQ=255)
                                                                            ggCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGc                                                             >  7:34775/1‑149 (MQ=255)
                                                                            ggCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGc                                                             >  7:39559/1‑149 (MQ=255)
                                                                                  gtgtGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAg                                                       >  2:305009/1‑149 (MQ=255)
                                                                                       gTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATgg                                                  >  3:135828/1‑149 (MQ=255)
                                                                                         cGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCt                                                >  2:303250/1‑149 (MQ=255)
                                                                                          gAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                               <  1:185842/149‑1 (MQ=255)
                                                                                          gAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                               <  8:109106/149‑1 (MQ=255)
                                                                                            ggCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTggg                                             >  5:194812/1‑149 (MQ=255)
                                                                                               gcAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTg                                          >  6:300577/1‑149 (MQ=255)
                                                                                               gcAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTg                                          >  2:200966/1‑149 (MQ=255)
                                                                                                     gctgctGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAg                                     <  1:151869/148‑1 (MQ=255)
                                                                                                      ctgctgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGt                                   <  6:272496/149‑1 (MQ=255)
                                                                                                        gctgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTg                                  >  8:199648/1‑148 (MQ=255)
                                                                                                         ctgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGaa                                >  3:275979/1‑149 (MQ=255)
                                                                                                          tgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAAt                               >  8:136129/1‑149 (MQ=255)
                                                                                                          tgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAAt                               >  7:95514/1‑149 (MQ=255)
                                                                                                          tgGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCAACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTAGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAAt                               >  7:236078/1‑149 (MQ=255)
                                                                                                            gggCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAAtt                              <  7:136129/148‑1 (MQ=255)
                                                                                                               cAGATCCTGGTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGt                          <  5:149367/149‑1 (MQ=255)
                                                                                                               cAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGt                          <  3:292611/149‑1 (MQ=255)
                                                                                                                aGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                               <  3:175227/127‑1 (MQ=255)
                                                                                                                aGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTg                                               >  4:175227/1‑127 (MQ=255)
                                                                                                                 gATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGc                        <  7:37343/149‑1 (MQ=255)
                                                                                                                  aTCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTg                         >  1:191778/1‑147 (MQ=255)
                                                                                                                  aTCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTg                         <  3:295774/147‑1 (MQ=255)
                                                                                                                  aTCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTg                         >  4:295774/1‑147 (MQ=255)
                                                                                                                    ccTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAgg                     >  6:216272/1‑149 (MQ=255)
                                                                                                                      tgctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAggcg                   >  6:127704/1‑149 (MQ=255)
                                                                                                                       gctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGt                                   >  8:224950/1‑132 (MQ=255)
                                                                                                                       gctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGt                                   <  7:224950/132‑1 (MQ=255)
                                                                                                                       gctgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAggcgg                  >  5:246903/1‑149 (MQ=255)
                                                                                                                         tgGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGcc                <  3:101004/149‑1 (MQ=255)
                                                                                                                            aTGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGtt             <  1:303250/149‑1 (MQ=255)
                                                                                                                             tGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGtt             >  2:236520/1‑148 (MQ=255)
                                                                                                                               ggCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCa           <  5:127704/148‑1 (MQ=255)
                                                                                                                                gCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCAc          >  6:112462/1‑148 (MQ=255)
                                                                                                                                ccGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCAc          >  3:33654/2‑148 (MQ=255)
                                                                                                                                 cGTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTa        >  3:110130/1‑149 (MQ=255)
                                                                                                                                  gTGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTAc       <  4:33654/149‑1 (MQ=255)
                                                                                                                                  gTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTAc       <  4:135828/149‑1 (MQ=255)
                                                                                                                                   tcgCATCCAGCAGGATGCCACGGATGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGACATCGCGCACAACAAGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACg      >  4:116089/3‑149 (MQ=255)
                                                                                                                                   tGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGt                                   <  3:135011/120‑1 (MQ=255)
                                                                                                                                   tGGCATCCTGCTGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGt                                   >  4:135011/1‑120 (MQ=255)
                                                                                                                                    ggCATCCAGCAGGAGGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGc     >  7:263747/1‑149 (MQ=255)
                                                                                                                                       aTCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCg  >  3:23129/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                             
GTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGTGGCAGTGCCATGGCGCACAACATGCTCGGGCGTTGCCTGGAGCATGGCTGGGGTGGCGAGGTGAATTTGGTGCAGGCGGCCGTTCACTACGCCCG  >  NC_002947/1001054‑1001337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑