Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,025,788 C→A 15.0% A394S (GCG→TCG) ‡ dppA‑II ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,025,7880CA15.0% 97.8 / 10.1 40A394S (GCG→TCG) ‡dppA‑IIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (13/21);  new base A (3/3);  total (16/24)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.28e-01

CCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTTGATG  >  NC_002947/1025647‑1025936
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ccagccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCACATTCGTAGCTGACGAGTCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAg                                                                                                                                                >  8:222271/1‑148 (MQ=38)
  agccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaa                                                                                                                                             <  2:198659/149‑2 (MQ=255)
   gccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaag                                                                                                                                            >  6:230736/1‑147 (MQ=255)
   gccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaag                                                                                                                                            <  5:230736/149‑3 (MQ=255)
     cagTTGGCCGGGTCGCCGTTGTCAGCGGTCGAGCGGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGATCTTGCTCCAGGCGGACTGGAGCaag                                                                                                                                            <  7:222271/147‑3 (MQ=255)
       gTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCgg                                                                                                                                        >  1:269288/1‑149 (MQ=255)
              gggTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAg                                                                                                                                 >  1:145851/1‑149 (MQ=255)
               ggTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGc                                                                                                                                <  8:71314/149‑1 (MQ=255)
                   gCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTg                                                                                                                             <  5:164672/148‑1 (MQ=255)
                    ccGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGc                                                                                                                           <  8:6264/149‑1 (MQ=255)
                      gTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACTATGCGCACATTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGAATTTCGGCAATCAGATTGGCAt                                                                                                                         >  4:237790/1‑149 (MQ=255)
                         gTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTg                                                                                                                       <  7:590/148‑1 (MQ=255)
                          tCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTgg                                                                                                                      <  3:237790/148‑1 (MQ=255)
                            aCCGGTCCAGGGGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCGATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTgggg                                                                                                                    <  7:123868/148‑1 (MQ=255)
                            aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTgggg                                                                                                                    <  1:114912/148‑1 (MQ=255)
                               ggTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGt                                                                                                                <  2:145851/149‑1 (MQ=255)
                                    aGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACggg                                                                                                           >  5:291411/1‑149 (MQ=255)
                                         aTCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTg                                                                                                      <  6:193805/149‑1 (MQ=255)
                                              ggCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCg                                                                                                 <  2:17914/149‑1 (MQ=255)
                                                          cTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCca                                                                                       >  8:71817/1‑147 (MQ=255)
                                                             aCCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAggg                                                                                  <  2:269288/149‑1 (MQ=255)
                                                                       aTGCGCTTGAGGTATTCTCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCCGCCTGTAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGt                                                                        <  2:114339/149‑1 (MQ=255)
                                                                           gcTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTTATCTCGGTGCCa                                                                    <  6:291411/149‑1 (MQ=255)
                                                                             ttGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAgg                                                                  >  2:58917/1‑149 (MQ=255)
                                                                               gAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGtt                                                                                                       <  7:230000/110‑1 (MQ=255)
                                                                               gAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGtt                                                                                                       >  8:230000/1‑110 (MQ=255)
                                                                                aGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGttt                                                               <  3:277412/149‑1 (MQ=255)
                                                                                  gTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGa                                                             >  1:11000/1‑149 (MQ=255)
                                                                                         ccccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGa                                                                                                                                                 >  5:258803/1‑58 (MQ=255)
                                                                                         ccccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGa                                                                                                                                                 <  6:258803/58‑1 (MQ=255)
                                                                                                    gCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCa                                           <  4:285137/149‑1 (MQ=255)
                                                                                                         cGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGt                                      <  2:11000/149‑1 (MQ=255)
                                                                                                           aTGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCt                                    <  2:139501/149‑1 (MQ=255)
                                                                                                              cgcACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGc                                 <  7:71817/149‑1 (MQ=255)
                                                                                                                    ttGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTc                           >  8:190205/1‑149 (MQ=255)
                                                                                                                             aGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTa                  >  1:18085/1‑149 (MQ=255)
                                                                                                                              aaatGCTCCAGGCGGACTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTAc                 >  2:55421/4‑149 (MQ=255)
                                                                                                                                  gCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACggg              <  3:293050/148‑1 (MQ=255)
                                                                                                                                      cAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTc         >  6:151739/1‑149 (MQ=255)
                                                                                                                                       aGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTcc        >  7:272936/1‑149 (MQ=255)
                                                                                                                                             ccTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTTGATg  >  1:85944/1‑149 (MQ=255)
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CCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTTGATG  >  NC_002947/1025647‑1025936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑