Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,041,942	T→G	10.8%	V142G (GTT→GGT)	colR →	two‑component system response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,041,942	0	T	G	10.8%	173.6 / 14.1	65	V142G (GTT→GGT)	colR	two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base T (25/33);  new base G (3/4);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

CTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGCCTG  >  NC_002947/1041796‑1042087                                                                                                                                                    |                                                                                                                                                  cTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTa                                                                                                                                                 >  7:69582/1‑149 (MQ=255)    gggggCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCA‑GGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTaac                                                                                                                                               <  1:199476/147‑3 (MQ=255)        gCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCa                                                                                                                                          <  7:208129/149‑1 (MQ=255)          tGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAgg                                                                                                                                        <  7:182809/149‑1 (MQ=255)          tGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAgg                                                                                                                                        >  3:21790/1‑149 (MQ=255)            acgacTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAggg                                                                                                                                       <  5:271495/148‑1 (MQ=255)            acgacTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAgg                                                                                                                                        <  7:245421/147‑1 (MQ=255)              gacTATCTGCTCCAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCg                                                                                                                                    <  7:136895/149‑1 (MQ=255)                   ccTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAGGGtatcga                                                                                                                                 <  3:210404/147‑6 (MQ=255)                   ccTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTg                                                                                                                                <  7:168350/148‑1 (MQ=255)                       cTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTg                                                                                                                             >  2:288249/1‑147 (MQ=255)                          aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGCCTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAg                                                                                                                          <  1:91252/148‑1 (MQ=255)                          aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGc                                                                                                                         >  6:61797/1‑148 (MQ=255)                          aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCt                                                                                                                        >  6:197500/1‑149 (MQ=255)                           aGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCt                                                                                                                                 >  1:68540/1‑139 (MQ=255)                           aGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCt                                                                                                                                 <  2:68540/139‑1 (MQ=255)                            gCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCa                                                                                                                      <  2:98267/149‑1 (MQ=255)                              cATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAAc                                                                                                                    <  4:72379/149‑1 (MQ=255)                                   gCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGt                                                                                                               >  8:190362/1‑149 (MQ=255)                                     ccTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCg                                                                                                             >  2:131551/1‑149 (MQ=255)                                         tCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGc                                                                                                           <  4:69027/147‑1 (MQ=255)                                                tGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAgctg                                                                                                  >  3:110592/1‑149 (MQ=255)                                                 ggcggcGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAgctgc                                                                                                 >  2:277747/1‑149 (MQ=255)                                                  gcggcgCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAgctgct                                                                                                >  1:143330/1‑149 (MQ=255)                                                    ggcgCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTgg                                                                                              <  1:131551/149‑1 (MQ=255)                                                       gcgcATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGtatcga                                                                                                                                 <  1:109345/111‑6 (MQ=255)                                                       gcgcATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGtatcga                                                                                                                                 >  2:109345/1‑106 (MQ=255)                                                        cgcATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGt                                                                                          <  4:21790/149‑1 (MQ=255)                                                           aTCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACt                                                                                       >  8:158705/1‑149 (MQ=255)                                                             cGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAC‑GAGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTg                                                                                      >  4:4239/1‑148 (MQ=255)                                                              gAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGa                                                                                     <  7:190362/148‑1 (MQ=255)                                                               aaGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTgg                                                                                              >  6:303561/1‑138 (MQ=255)                                                               aaGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTgg                                                                                              <  5:303561/138‑1 (MQ=255)                                                                       ctgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGaaaa                                                                            <  1:288249/148‑1 (MQ=255)                                                                       ctgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGaaaa                                                                            <  5:61797/148‑1 (MQ=255)                                                                            ccGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGGAAAGCCCGc                                                                      <  5:207707/149‑1 (MQ=255)                                                                                   cAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACt                                                               <  5:216624/149‑1 (MQ=255)                                                                                          gTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGcgccgc                                                        >  2:50486/1‑149 (MQ=255)                                                                                           tGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGaa                                                                              <  2:19186/126‑1 (MQ=255)                                                                                           tGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGaa                                                                              >  1:19186/1‑126 (MQ=255)                                                                                            gggCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGa                                                      <  5:197500/149‑1 (MQ=255)                                                                                             ggcggcGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTTCTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAg                                                     <  1:50486/149‑1 (MQ=255)                                                                                                   gcACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTg                                               <  7:158705/149‑1 (MQ=255)                                                                                                           cAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACt                                                               >  8:121491/1‑125 (MQ=255)                                                                                                           cAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACt                                                               <  7:121491/125‑1 (MQ=255)                                                                                                           cAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCTAGGTGCTGGaagaag                                        <  8:69582/148‑1 (MQ=255)                                                                                                           cAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGgaagaa                                         <  1:277747/147‑1 (MQ=255)                                                                                                                   cTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTgg                                >  2:133651/1‑149 (MQ=255)                                                                                                                     gACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTgggg                              <  3:4239/149‑1 (MQ=255)                                                                                                                           aGCTACGTCCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGcgac                          <  2:143330/147‑1 (MQ=255)                                                                                                                           aGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGaaaa                                                                            >  5:24894/1‑97 (MQ=255)                                                                                                                           aGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGaaaa                                                                            <  6:24894/97‑1 (MQ=255)                                                                                                                             cTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTtgtg                                 >  4:193070/1‑138 (MQ=255)                                                                                                                             cTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTtgtg                                 <  3:193070/138‑1 (MQ=255)                                                                                                                              tACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTg                     >  3:285671/1‑149 (MQ=255)                                                                                                                                     tCGATACACTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACACGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGa               >  1:101590/1‑148 (MQ=255)                                                                                                                                      cGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGa               >  2:303642/1‑147 (MQ=255)                                                                                                                                       gATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATa             <  4:110592/148‑1 (MQ=255)                                                                                                                                           cccTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGAc        <  1:133651/149‑1 (MQ=255)                                                                                                                                            ccTGGCGGGTAACCTCCAGGGTCGCCTGTTGAAGCTCCACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCTGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGAGACGACTGCCCTGATAGCGa         >  2:104336/1‑147 (MQ=255)                                                                                                                                            ccTGGCGGGTAACCTCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGc           >  5:133633/1‑145 (MQ=255)                                                                                                                                            ccTGGCGGGTAACCTCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGc           <  6:133633/145‑1 (MQ=255)                                                                                                                                            ccTGGCGGGTAACCTCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGa         >  4:209929/1‑147 (MQ=255)                                                                                                                                             cTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAg      >  2:283179/1‑149 (MQ=255)                                                                                                                                                 aGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGCCTg  <  4:285671/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAG‑GTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGCCTG  >  NC_002947/1041796‑1042087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑