Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,046,227 C→G 8.7% M717I (ATG→ATC)  PP_0906 ← RND family multidrug transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,046,2270CG 8.7% 162.6 / 3.8 56M717I (ATG→ATC) PP_0906RND family multidrug transporter
Reads supporting (aligned to +/- strand):  ref base C (29/22);  new base G (2/3);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GTTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCCAGCAGTGCCG  >  NC_002947/1046092‑1046375
                                                                                                                                       |                                                                                                                                                    
gttgttCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACAccccc                                                                                                                                          <  6:141366/148‑1 (MQ=255)
 ttgttCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGGGGGTGTCGATGGACAccctcgg                                                                                                                                        <  1:72760/149‑5 (MQ=255)
     tCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTgc                                                                                                                                    >  7:280520/1‑149 (MQ=255)
         ccagccGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGcgccg                                                                                                                                >  2:259173/1‑149 (MQ=255)
             ccGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTgc                                                                                                                                    <  4:244541/141‑1 (MQ=255)
             ccGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTgc                                                                                                                                    >  3:244541/1‑141 (MQ=255)
             ccGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCtt                                                                                                                            >  2:150681/1‑149 (MQ=255)
              cGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCttt                                                                                                                           >  7:9357/1‑149 (MQ=255)
               ggggTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTg                                                                                                                          <  1:259173/149‑1 (MQ=255)
                   ttGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCTCg                                                                                                                      >  7:273128/1‑149 (MQ=255)
                      tCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAGCGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCg                                                                                                                      <  4:264791/146‑1 (MQ=255)
                      tCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAGCGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCg                                                                                                                      >  3:264791/1‑146 (MQ=255)
                        gcgGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGa                                                                                                                 >  6:14586/1‑149 (MQ=255)
                            tAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGTAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTc                                                                                                             <  1:6315/149‑1 (MQ=255)
                             aaGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTcc                                                                                                            >  7:159959/1‑149 (MQ=255)
                              aGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTcc                                                                                                            <  5:14586/148‑1 (MQ=255)
                                     tCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGCCGCCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCcacaaccac                                                                                                    >  7:85364/1‑149 (MQ=255)
                                          cggggCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCgg                                                                                               <  6:7540/147‑1 (MQ=255)
                                          cTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCgg                                                                                               <  8:159959/149‑1 (MQ=255)
                                                    aCCGTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGCGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGaa                                                                                     <  5:5850/149‑1 (MQ=255)
                                                     ccTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAg                                                                                    <  2:132372/149‑1 (MQ=255)
                                                     ccTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAg                                                                                    <  8:9357/149‑1 (MQ=255)
                                                      cTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGac                                                                                                                                                <  7:276541/88‑1 (MQ=255)
                                                      cTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGac                                                                                                                                                >  8:276541/1‑88 (MQ=255)
                                                      cTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCt                                                                                                                             >  3:80556/1‑107 (MQ=255)
                                                      cTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCt                                                                                                                             <  4:80556/107‑1 (MQ=255)
                                                        tGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGc                                                                                  >  5:28318/1‑148 (MQ=255)
                                                            aCTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGAt                                                                             <  3:203248/149‑1 (MQ=255)
                                                             cTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTccacga                                                                                                        <  4:301127/121‑3 (MQ=255)
                                                             cTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTccacca                                                                                                        >  3:301127/1‑121 (MQ=255)
                                                                cGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGa                                                                          >  7:258992/1‑148 (MQ=255)
                                                                 ggCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACCCCACCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGCGTTTGTAGAAGGCAAGATCGata                                                                        >  3:60273/1‑149 (MQ=255)
                                                                    cTTCAAGGGTGAAAGGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATc                                                                      >  7:262693/1‑148 (MQ=255)
                                                                    cTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCTCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCg                                                                     <  8:273128/149‑1 (MQ=255)
                                                                        aaGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAgg                                                                  <  1:150681/148‑1 (MQ=255)
                                                                              gAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCg                                                            >  5:68336/1‑148 (MQ=255)
                                                                               aaaCGGTTGATTTACGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGCCACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGCCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGaa                                                          >  7:158069/1‑149 (MQ=255)
                                                                                               ccTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGa                                          <  8:262693/149‑1 (MQ=255)
                                                                                                   gCCCAGCAGGGTCGCCAATGTGCCGCCGAGGGTGTCCATCGACACCCCCTTCTGCGCCGCCTTTGCCCGGTCGTTGTCCACCAACACTTCGGGTTTGTCGATGTAAAGATCGATATCTAGGAAGGCGAACTTGCCCGATTCCCGAgc                                        <  4:60273/147‑1 (MQ=255)
                                                                                                     ccAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAATGCAAGATCGATATCGAGGAAGGCGAACTTGCACGATTACTGAgcgcgc                                    >  8:206279/1‑149 (MQ=255)
                                                                                                     ccAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAgcgcgc                                    >  4:55360/1‑149 (MQ=255)
                                                                                                            ggTCGCCAAGATGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGa                             >  8:132214/1‑149 (MQ=255)
                                                                                                             gTCGCCAAGATGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGAt                            <  7:132214/149‑1 (MQ=255)
                                                                                                                gCCAAGGTGCCGCCGCTGGTGTCCATCGACAACCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCg                                                            <  2:181213/114‑1 (MQ=255)
                                                                                                                gCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGTTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCTAGATCGAGATCGAGGAAgggg                                                            >  1:181213/1‑112 (MQ=255)
                                                                                                                gCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCg                         >  2:229912/1‑149 (MQ=255)
                                                                                                                   aaGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTg                      <  8:280520/149‑1 (MQ=255)
                                                                                                                           gccgAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGt              >  1:11666/1‑149 (MQ=255)
                                                                                                                           gccgAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGt              >  5:120006/1‑149 (MQ=255)
                                                                                                                              gggggTGTCGATGGACACCCTCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAg                                                               >  5:147055/3‑97 (MQ=255)
                                                                                                                              gggggTGTCGATGGACACCCTCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAg                                                               <  6:147055/95‑1 (MQ=255)
                                                                                                                                gggTGTCGATGGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTg                      >  8:143135/1‑136 (MQ=255)
                                                                                                                                gggTGTCGATGGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTg                      <  7:143135/136‑1 (MQ=255)
                                                                                                                                 ggTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCcag          >  4:107435/1‑147 (MQ=255)
                                                                                                                                 ggTGTCCATCGACACCCCCATCTGCGCCGCCTTTGACCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCcag          >  6:82427/1‑147 (MQ=255)
                                                                                                                                     tCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCCAGCAGTGc    >  3:187373/1‑149 (MQ=255)
                                                                                                                                       cATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTCGGCCACGTCCAGCAGTGCCg  >  5:297821/1‑149 (MQ=255)
                                                                                                                                       |                                                                                                                                                    
GTTGTTCAGCCAGCCGGGGTTGTCGCGGTAAGGCCGTTCAACCTGGGCGATCACCTTGTAACTGCGGCCTTCAAGGGTGAAACGGTTGATTTCCGCCTCGCCCAGCAGGGTCGCCAAGGTGCCGCCGAGGGTGTCCATCGACACCCCCATCTGCGCCGCCTTTGCCCGGTCGATGTCCACCACCACTTCGGGTTTGTCGAAGGCAAGATCGATATCGAGGAAGGCGAACTTGCCCGATTCCTGAGCGCGCGTCTTGATGCGTTGGGCCACGTCCAGCAGTGCCG  >  NC_002947/1046092‑1046375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑