| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,071,037 | G→C | 6.8% | L117V (CTG→GTG) | PP_0928 ← | K+‑dependent Na+/Ca+ exchanger‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,071,037 | 0 | G | C | 6.8% | 188.7 / 4.5 | 59 | L117V (CTG→GTG) | PP_0928 | K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base G (27/28); new base C (2/2); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GCGTACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGTTGCTGC > NC_002947/1070893‑1071183 | gcGTACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTgg < 1:186361/149‑1 (MQ=255)gcGTACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTgg < 8:9659/149‑1 (MQ=255) tACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCa > 4:78016/1‑149 (MQ=255) tACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCa > 4:75866/1‑149 (MQ=255) cTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTg > 8:192208/1‑149 (MQ=255) cTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTg > 7:100606/1‑149 (MQ=255) aCCAGAAGCGCCCGGCCCCGGAGCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCACCTGGGCagg > 7:194614/1‑143 (MQ=255) aCCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCACCTGGGCagg < 8:194614/145‑3 (MQ=255) ccAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCgg < 6:204177/149‑1 (MQ=255) cAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGa < 1:224247/149‑1 (MQ=255) cAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGa > 3:8585/1‑149 (MQ=255) cAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGa > 4:219098/1‑149 (MQ=255) gcgcCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGAc < 6:230976/145‑1 (MQ=255) gcgcCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGAc > 5:230976/1‑145 (MQ=255) gcCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCa < 6:120852/149‑1 (MQ=255) ccGGCCCCGGACCTGGCGGCGGCCGGGGGAGGGTAGGTGCGTGGGGAATGGCGGGAATGATGCCATAGCATTGCCAGGTAACCGGCCAGTCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATa < 4:43749/149‑1 (MQ=255) gCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCAGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATacac < 2:35360/149‑1 (MQ=255) cGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCACACACCAGc < 2:157257/149‑1 (MQ=255) cTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCt < 3:78016/149‑1 (MQ=255) cTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCt < 3:75866/149‑1 (MQ=255) ccgTCCGGGGCAGGGTAGGTGCTTGCGTAATGGCGCGACTGATGCCAGAGAATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTCACCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACACCGCATACACAAGCCCGCTGGCAAg > 2:97247/2‑149 (MQ=255) gggCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATacac > 4:264078/1‑127 (MQ=255) gggCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATacac < 3:264078/127‑1 (MQ=255) cgcgACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTg < 8:100606/149‑1 (MQ=255) gcgACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCTGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTgg < 1:97247/149‑1 (MQ=255) gACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCg > 3:42689/1‑149 (MQ=255) gACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCg < 2:24992/149‑1 (MQ=255) aCTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGt < 5:280945/149‑1 (MQ=255) tGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGa > 2:143096/1‑149 (MQ=255) gATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGa > 6:111812/1‑148 (MQ=255) aTGCCAGTGCATTGCCAGGTAAGCGGGCAGCCGCAGGAATAGCAGTGCCCCTTCAACGCTGCGCAGGTGGGCATTGGCGGACTGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTg < 1:70474/146‑1 (MQ=255) gCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACAcc > 5:305635/1‑149 (MQ=255) ccAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACAccc < 1:143096/149‑1 (MQ=255) cAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCg > 4:263060/1‑149 (MQ=255) agagCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGTCAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCg > 8:138806/1‑148 (MQ=255) cATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGatc < 4:274075/103‑1 (MQ=255) cATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGatc > 3:274075/1‑103 (MQ=255) ccAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGgat > 2:279094/1‑148 (MQ=255) ccAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGgat < 4:42689/148‑1 (MQ=255) cAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCa < 3:263060/149‑1 (MQ=255) ccAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGcc > 2:138347/1‑148 (MQ=255) cAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCga < 3:219098/149‑1 (MQ=255) cAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCga < 1:279094/149‑1 (MQ=255) cAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCga < 4:8585/149‑1 (MQ=255) aaTAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAAt > 6:51797/1‑149 (MQ=255) aTAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAAt > 5:61163/1‑148 (MQ=255) ccccTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACg > 2:14242/1‑149 (MQ=255) ccTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGtt > 5:47910/1‑149 (MQ=255) ttCAACCCTGCCCAGGTGGGCATTGGTGGACAGCGCATACAACAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTg > 2:80991/1‑148 (MQ=255) aaCCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAg < 3:292980/148‑1 (MQ=255) ccaatGCCCACCTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGc < 8:164868/120‑1 (MQ=255) ccaatGCCCACCTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGc > 7:164868/5‑124 (MQ=255) aCCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGAt < 6:61163/149‑1 (MQ=255) cccTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAAt > 8:97898/1‑130 (MQ=255) cccTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATg > 6:115443/1‑149 (MQ=255) ccTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGt > 2:307494/1‑149 (MQ=255) cTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGtt > 4:48951/1‑149 (MQ=255) cccAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGTtg < 1:307494/147‑1 (MQ=255) cAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGTtgctgc < 3:48951/149‑1 (MQ=255) | GCGTACCTGACCAGAAGCGCCCGGCCCCGGACCTGGCGGCGTCCGGGGCAGGGTAGGTGCGTGCGTAATGGCGCGACTGATGCCAGAGCATTGCCAGGTAACCGGCCAGCCCCAGCAATAGCAGTGCCCCTTCAACCCTGCCCAGGTGGGCATTGGCGGACAGCGCATACACCAGCCCGCTGGCAAGGATCATCAGGGGAATGTCCAGCCGCACCAGCTGGCGTGACACCCGCAGCGGGATGATCAGCGCGGCCAGGCCGAGAATGACCAGTACGTTGAAGATGTTGCTGC > NC_002947/1070893‑1071183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |