| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,104,396 | T→C | 6.8% | V385V (GTT→GTC) | murA → | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,104,396 | 0 | T | C | 6.8% | 170.6 / 2.1 | 59 | V385V (GTT→GTC) | murA | UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (26/29); new base C (2/2); total (28/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCAAGGACGCACCTTTTGA > NC_002947/1104254‑1104538 | tttACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGCCCTGTGGGTCCTACCCCCAGCCTGGTGAGGTCGCCGCTGT‑TTGcc > 1:102357/1‑148 (MQ=255)tttACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TCGCCg > 7:175190/1‑147 (MQ=255) aCGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAgg < 1:169192/148‑1 (MQ=255) cACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATAccc < 2:92540/148‑1 (MQ=255) cGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTgat < 8:115186/149‑1 (MQ=255) ggCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTgatcgatc < 8:22159/148‑1 (MQ=255) gcgcgcAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGatcgatcg > 4:215635/1‑148 (MQ=255) gcgcgcAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGc < 2:66423/149‑1 (MQ=255) aGATCCAGGCTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTAc < 8:195879/149‑1 (MQ=255) aGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATc < 6:14792/149‑1 (MQ=255) aGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATc > 4:30943/1‑149 (MQ=255) ggTTGAAGGCAACACCGCCATCGTCTCTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGGATCTACCACATc > 8:262753/1‑148 (MQ=255) gTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGa > 7:190374/1‑149 (MQ=255) gTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGa < 3:198484/149‑1 (MQ=255) gTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGa > 4:47120/1‑149 (MQ=255) gAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTgat < 1:229727/125‑1 (MQ=255) aGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTg > 2:107842/1‑149 (MQ=255) ggCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTgg > 2:181517/1‑149 (MQ=255) ggCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTgg > 2:98095/1‑149 (MQ=255) gaaCACCGCCATCGTCACTGGGGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGTTGCTGTCGGCGCTGGCTTGCCGAAGGCGATACCCTGATCGATCGc < 1:22477/127‑1 (MQ=255) cAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGc > 2:22477/1‑127 (MQ=255) cacCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTAc > 5:245169/1‑148 (MQ=255) acCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGa < 7:151605/149‑1 (MQ=255) acCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TCGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGa < 8:175190/149‑1 (MQ=255) cGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACAGTGGTAACGAGt > 3:145352/1‑149 (MQ=255) ccATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGc < 1:181517/149‑1 (MQ=255) ccATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGc < 6:245169/149‑1 (MQ=255) gTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGa < 3:30943/149‑1 (MQ=255) aCTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAgg < 7:87720/148‑1 (MQ=255) aaGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCa < 3:215635/149‑1 (MQ=255) aaGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCCGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCa < 4:114332/149‑1 (MQ=255) tGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTc > 7:292765/1‑149 (MQ=255) gAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCg > 8:171171/1‑149 (MQ=255) aaGGGTGCCCCGGGCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCTATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCgg < 2:102357/149‑1 (MQ=255) tGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGcgcg > 6:92364/1‑148 (MQ=255) ccccGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATAccc > 8:12128/1‑75 (MQ=255) ccccGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATAccc < 7:12128/75‑1 (MQ=255) ggTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATcc < 8:190374/149‑1 (MQ=255) aTGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATccgccg > 6:262955/1‑149 (MQ=255) gCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCcgcg > 8:13521/1‑148 (MQ=255) gCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCCGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACTACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTc > 7:135009/1‑129 (MQ=255) gCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCCGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACTACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTc < 8:135009/129‑1 (MQ=255) ccACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTa > 6:31380/1‑149 (MQ=255) aCC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACACTGATCGATCGCATCTACCACATCGA‑CGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGc > 5:66091/1‑148 (MQ=255) gCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAg > 8:283138/1‑148 (MQ=255) gCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGaaa < 8:178099/123‑1 (MQ=255) cTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTAcccc > 2:42327/1‑149 (MQ=255) ccGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCcgcg > 3:187426/1‑149 (MQ=255) cGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCgg < 7:171171/149‑1 (MQ=255) ccAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGt < 5:31380/149‑1 (MQ=255) ccTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCaa < 1:98095/149‑1 (MQ=255) gTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCAAgg < 5:262955/147‑1 (MQ=255) cTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCAAGGACGCa > 3:8153/1‑149 (MQ=255) tCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCAAGGACGCAc < 1:42327/147‑1 (MQ=255) tCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCAAGGACGCACCt < 7:262753/149‑1 (MQ=255) ggCTCTGG‑TTGACGAAGGCGATACCCTGATCGATCGCATCTACCACATAGACAGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTAGGCTCGAAAATCCTCCGCGTACCGTGCTAGTACCCAGCGGGTGCAAGGACGCACCttt > 8:112537/1‑149 (MQ=255) gcgcTGG‑TCGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCcgc > 8:167219/1‑128 (MQ=255) gcgcTGG‑TCGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCcgc < 7:167219/128‑1 (MQ=255) cgcTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGA‑CGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCAAGGACGCACCTTttga < 6:66091/149‑1 (MQ=255) | TTTACGAAATGCACCGCATGGGCGCGCAGATCCAGGTTGAAGGCAACACCGCCATCGTCACTGGCGTCAAGGCGCTGAAGGGTGCCCCGGTCATGGCCACCGACC‑‑TGCGTGCTTCCGCCAGCCTGGTGCTGTCGGCGCTGG‑TTGCCGAAGGCGATACCCTGATCGATCGCATCTACCACATCGACCGTGGTTACGAGTGCATCGAGGAAAAACTGCAGATGCTCGGCGCGAAAATCCGCCGCGTACCGGGCTAGTACCCCGCGGGTGCAAGGACGCACCTTTTGA > NC_002947/1104254‑1104538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |