Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,109,140	T→A	6.6%	D38V (GAC→GTC)	PP_0968 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,109,140	0	T	A	6.6%	203.8 / 2.8	72	D38V (GAC→GTC)	PP_0968	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/28);  new base A (2/3);  total (40/32)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

ATCCCCCCGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCT  >  NC_002947/1109002‑1109287                                                                                                                                           |                                                                                                                                                     aTCCCCCCGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCgg                                                                                                                                            >  8:266311/1‑149 (MQ=255) aTCCCCCCGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCgg                                                                                                                                            >  6:107283/1‑149 (MQ=255)  tCCCCCCGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCTGTGACccggcccggc                                                                                                                                           <  1:208773/149‑1 (MQ=255)    cccccGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCt                                                                                                                                          <  8:143584/148‑1 (MQ=255)      cccGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTg                                                                                                                                         >  7:75550/1‑147 (MQ=255)       ccGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAg                                                                                                                                      >  6:187919/1‑149 (MQ=255)        cGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGc                                                                                                                                        >  6:298850/1‑146 (MQ=255)        cGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGc                                                                                                                                        <  5:298850/146‑1 (MQ=255)            gTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCTGTGACccggcccggca                                                                                                                                          <  6:272323/140‑2 (MQ=255)            gTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCTGTGACccggcccggca                                                                                                                                          >  5:272323/1‑139 (MQ=255)            gTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCAGGGCCGGGTCACAGCCCGGCTGCAGGGc                                                                                                                                   >  1:113807/1‑147 (MQ=255)                            tGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAg                                                                                                                 >  7:112947/1‑149 (MQ=255)                                  cGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCCGCAGGGCCAGGAACAGCAATAACAGCA‑AACt                                                                                                          >  8:101228/1‑149 (MQ=255)                                   gCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACtt                                                                                                         >  3:294513/1‑149 (MQ=255)                                   gCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACtt                                                                                                         >  6:161169/1‑149 (MQ=255)                                   gCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCCGCAGGGCCAGGAACAGCAATAACAGCA‑AACtt                                                                                                         <  7:101228/149‑1 (MQ=255)                                       tttCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGTCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGc                                                                                                       <  1:221702/147‑1 (MQ=255)                                       tttCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCAGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCa                                                                                                     <  2:113807/149‑1 (MQ=255)                                          cAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTc                                                                                                  >  6:188187/1‑149 (MQ=255)                                            ggTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGc                                                                                                <  5:161169/149‑1 (MQ=255)                                             gTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCa                                                                                               >  2:214443/1‑149 (MQ=255)                                                 gTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATggg                                                                                           <  4:43370/149‑1 (MQ=255)                                                 gTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATggg                                                                                           <  3:272925/149‑1 (MQ=255)                                                    gCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGt                                                                                         <  5:107283/148‑1 (MQ=255)                                                       tCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCa                                                                                     >  5:96810/1‑149 (MQ=255)                                                          cccAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATcc                                                                                  <  8:112947/149‑1 (MQ=255)                                                           ccAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATaa                                                                                                                    <  3:35921/115‑1 (MQ=255)                                                           ccAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATaa                                                                                                                    >  4:35921/1‑115 (MQ=255)                                                            cAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCtt                                                                                <  5:187919/149‑1 (MQ=255)                                                            cAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCtt                                                                                <  7:266311/149‑1 (MQ=255)                                                                 gCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAg                                                                           >  1:275666/1‑149 (MQ=255)                                                                         tgctgcAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACCGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAAc                                                                   >  4:211559/1‑149 (MQ=255)                                                                         tgctgcAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAAc                                                                   >  7:143878/1‑149 (MQ=255)                                                                           ctgcAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCACAACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAAc                                                                   <  1:214443/148‑1 (MQ=255)                                                                             gcAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGc                                                               >  2:11185/1‑149 (MQ=255)                                                                               aaTTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGt                                                             >  8:100847/1‑149 (MQ=255)                                                                                 ttGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTg                                                           >  6:149290/1‑149 (MQ=255)                                                                                    cACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGcaa                                                        >  4:91162/1‑149 (MQ=255)                                                                                     aCGCCGTCAAGGCTGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAAAAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGcaa                                                        >  3:286191/1‑148 (MQ=255)                                                                                     aCGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAAt                                                                                                                      >  4:42961/1‑87 (MQ=255)                                                                                     aCGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAAt                                                                                                                      <  3:42961/87‑1 (MQ=255)                                                                                     aCGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGc                                                                          <  7:253115/130‑1 (MQ=255)                                                                                     aCGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGc                                                                          >  8:253115/1‑130 (MQ=255)                                                                                     aCGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGcaac                                                       <  6:96810/149‑1 (MQ=255)                                                                                       gCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGcaaca                                                      >  5:23587/1‑148 (MQ=255)                                                                                       gCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGcaaca                                                      >  1:96774/1‑148 (MQ=255)                                                                                           tCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGt                                                             <  7:126259/137‑1 (MQ=255)                                                                                           tCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGt                                                             >  8:126259/1‑137 (MQ=255)                                                                                           tCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTg                                                 >  4:283207/1‑149 (MQ=255)                                                                                             aaGGCTGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTgg                                                <  4:286191/148‑1 (MQ=255)                                                                                                         cAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGCCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATg                                            <  8:108092/140‑1 (MQ=255)                                                                                                         cAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGCCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATg                                            >  7:108092/1‑140 (MQ=255)                                                                                                             gTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTgg                                 >  4:221436/1‑147 (MQ=255)                                                                                                                  aGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCAACAACTGGCAGaaa                           >  6:271712/1‑148 (MQ=255)                                                                                                                   gggCGGCTCGGGTGCCGGGCCGGGTAACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACAGGCAGAAATa                         >  6:277471/1‑149 (MQ=255)                                                                                                                    ggcggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATAc                        <  3:91162/149‑1 (MQ=255)                                                                                                                     gcggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTgg                                 <  5:11179/139‑1 (MQ=255)                                                                                                                     gcggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTgg                                 >  6:11179/1‑139 (MQ=255)                                                                                                                      cggcTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATAc                        >  1:129895/1‑147 (MQ=255)                                                                                                                            gggTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAggtgg                 <  1:11185/148‑1 (MQ=255)                                                                                                                            gggTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAggtgg                 <  5:188187/148‑1 (MQ=255)                                                                                                                             ggTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGtt               >  7:245348/1‑149 (MQ=255)                                                                                                                              gTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGttt              <  8:143878/149‑1 (MQ=255)                                                                                                                              gTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGtt               >  4:270601/1‑148 (MQ=255)                                                                                                                               agccgggctgtGACCCGGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGtt               >  7:208058/2‑147 (MQ=255)                                                                                                                               agccgggctgtGACCCGGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGtt               <  8:208058/146‑1 (MQ=255)                                                                                                                                 ccgggccgggTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTAcc           <  2:96774/149‑1 (MQ=255)                                                                                                                                   gggccgggTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACctc         >  3:103659/1‑149 (MQ=255)                                                                                                                                     gccgggTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCtt       >  3:36039/1‑149 (MQ=255)                                                                                                                                      ccgggTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGTATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCttt      <  4:294513/149‑1 (MQ=255)                                                                                                                                       cgggTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTg     <  4:103659/149‑1 (MQ=255)                                                                                                                                          gTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCt  <  3:33889/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                     ATCCCCCCGGCGTAAGCCTTGCCGTAGTGCAGCCGCTGTTTCAGGTTGGTCGCATCGCCCAATTGCAGGTACTGCTGCAATTGCACGCCGTCAAGGCCGATGAGCAAGGTTTTAGGGCGGCTCGGGTGCCGGGCCGGGTCACAGCCCGGCTGCAGGGCCAGGAACAGCAATAACAGCA‑AACTTGCCAGTCGCATGGGGTCGCATCCTTGAAAGCACGCAACGAGCGTTGCAACAAAGTGGCATGCCCACAACTGGCAGAAATACCAGGTGGTTTACCTCTTTGCCT  >  NC_002947/1109002‑1109287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑