Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,114,349 T→A 6.9% N153I (AAT→ATT)  rlmF ← ribosomal RNA large subunit methyltransferase F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,114,3490TA 6.9% 169.2 / 3.0 58N153I (AAT→ATT) rlmFribosomal RNA large subunit methyltransferase F
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base A (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

GCTGCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAGCGCGCGCACTTGGGCACCCC  >  NC_002947/1114204‑1114496
                                                                                                                                                 |                                                                                                                                                   
gctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTg                                                                                                                                                   <  5:108548/148‑1 (MQ=255)
 ctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGc                                                                                                                                                 <  3:35079/149‑1 (MQ=255)
    ccGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCt                                                                                                                                               <  8:190132/148‑1 (MQ=255)
          gTGGCTTCGTCGCGTGAGGCATGTAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCGGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGTCCAGGCCAATGGCCTgggcaaa                                                                                                                                        <  8:94885/149‑6 (MQ=255)
          gTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCAATGGCCTgggcaaa                                                                                                                                        <  6:195782/149‑6 (MQ=255)
           tGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGa                                                                                                                                         >  1:17464/1‑147 (MQ=255)
             gCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGc                                                                                                                                     >  6:155853/1‑149 (MQ=255)
             gCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGc                                                                                                                                     <  2:183129/149‑1 (MQ=255)
                  gtcgCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTgg                                                                                                                                >  5:69117/1‑149 (MQ=255)
                          aGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGc                                                                                                                         <  2:17464/148‑1 (MQ=255)
                             cATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGAGGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAgc                                                                                                                     <  5:68548/149‑1 (MQ=255)
                                    gggCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCg                                                                                                              >  7:10327/1‑149 (MQ=255)
                                     ggCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCgg                                                                                                             <  6:100480/149‑1 (MQ=255)
                                     ggCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCgg                                                                                                             <  5:110116/149‑1 (MQ=255)
                                      gCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCtt                                                                                                                                  >  2:5690/1‑127 (MQ=255)
                                      gCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCtt                                                                                                                                  <  1:5690/127‑1 (MQ=255)
                                        gggTTGCACAGGGTCAGGTCGAAGCGTTCGCCCTCCTGCATCTGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTc                                                                                                          <  1:100685/149‑1 (MQ=255)
                                          gTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGa                                                                                                        <  3:104547/149‑1 (MQ=255)
                                                    gtcaggtcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGCATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagcc                                                                                              <  5:264945/149‑1 (MQ=255)
                                                       aggtcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagccgag                                                                                           <  4:66869/149‑1 (MQ=255)
                                                        ggtcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagccgag                                                                                           >  8:146122/1‑148 (MQ=255)
                                                          tcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAgg                                                                                          >  1:195491/1‑147 (MQ=255)
                                                          tcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAgg                                                                                          >  2:251755/1‑147 (MQ=255)
                                                          tcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGaa                                                                                        <  5:155853/149‑1 (MQ=255)
                                                            aaaGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGATCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAgc                                                                                      >  7:124917/1‑149 (MQ=255)
                                                             aaGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAgcg                                                                                     >  8:249759/1‑149 (MQ=255)
                                                                cGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAgcgc                                                                                    >  3:195586/1‑147 (MQ=255)
                                                                    cGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCg                                                                               <  6:69117/148‑1 (MQ=255)
                                                                             gcagcaGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTg                                                                     >  4:23387/1‑149 (MQ=255)
                                                                               agcagGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTa                                                                   <  8:10327/149‑1 (MQ=255)
                                                                                 cagGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGc                                                                 >  2:67701/1‑149 (MQ=255)
                                                                                    gCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCca                                                              <  4:195586/149‑1 (MQ=255)
                                                                                         tGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTg                                                                     >  8:201318/1‑137 (MQ=255)
                                                                                         tGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTg                                                                     <  7:201318/137‑1 (MQ=255)
                                                                                           aGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccga                                                                                                 >  8:18192/1‑107 (MQ=255)
                                                                                           aGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGt                                                         <  1:67701/147‑1 (MQ=255)
                                                                                           aGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTg                                                        <  6:306845/148‑1 (MQ=255)
                                                                                              aTGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATa                                                    <  2:195491/149‑1 (MQ=255)
                                                                                               tgtgCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAg                                                   <  7:146122/149‑1 (MQ=255)
                                                                                                gtgCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCATTGGagat                                                   >  1:260196/1‑145 (MQ=255)
                                                                                                  gcgcTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTg                                                                     >  8:15733/1‑128 (MQ=255)
                                                                                                  gcgcTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTg                                                                     <  7:15733/128‑1 (MQ=255)
                                                                                                    gcTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATg                                                >  3:100005/1‑147 (MQ=255)
                                                                                                        ggTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTACGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTg                                          >  5:196877/1‑149 (MQ=255)
                                                                                                         gTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGt                                                         >  5:94556/1‑133 (MQ=255)
                                                                                                         gTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGt                                                         <  6:94556/133‑1 (MQ=255)
                                                                                                               ctgctgGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCa                                   >  7:112998/1‑149 (MQ=255)
                                                                                                                 gctgGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACg                                 >  1:148396/1‑149 (MQ=255)
                                                                                                                       gcAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATg                           <  8:112998/149‑1 (MQ=255)
                                                                                                                        cAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGt                          >  2:26180/1‑149 (MQ=255)
                                                                                                                               aTGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAgcgc                   <  3:23387/149‑1 (MQ=255)
                                                                                                                                   tatcgtccAGGCCAATGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCAGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAgcgcgcg                >  6:223248/7‑148 (MQ=255)
                                                                                                                                   cTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAgcgcgcgc               >  7:124714/1‑149 (MQ=255)
                                                                                                                                    tttGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAGCGCGCGCa              >  8:270791/1‑149 (MQ=255)
                                                                                                                                         ccAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAGCGCGCGCACTTgg         >  5:199796/1‑149 (MQ=255)
                                                                                                                                           aGGCCAATGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAGCGCGCGCACTTggg        >  5:43318/1‑148 (MQ=255)
                                                                                                                                               cATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAGCGCGCGCACTTGGGCAccc   >  7:256451/1‑149 (MQ=255)
                                                                                                                                                aTTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAGCGCGCGCACTTGGGCAcccc  <  1:251755/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GCTGCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTTTGCCCAGGCCATTGGCCTGGACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCAGCGCGCGCACTTGGGCACCCC  >  NC_002947/1114204‑1114496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑