| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,178,711 | A→G | 10.3% | intergenic (+16/+78) | guaA → / ← PP_1033 | glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,178,711 | 0 | A | G | 10.3% | 153.8 / 7.1 | 58 | intergenic (+16/+78) | guaA/PP_1033 | glutamine‑hydrolyzing GMP synthase/sulfatase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (24/28); new base G (3/3); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
CACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCC > NC_002947/1178576‑1178853 | cacCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGc < 5:138400/149‑1 (MQ=255) cTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGAGGTGTCGAGCAAGCCGCCTAACCGCAATAGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCCCAg > 1:99418/1‑149 (MQ=255) ccGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGAc < 8:78753/148‑1 (MQ=255) ccGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGT‑GCCAGCCTGGGCTGGCTACCCgatcac < 1:59012/147‑6 (MQ=255) cGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACg > 1:125958/1‑148 (MQ=255) gTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGa < 1:304856/149‑1 (MQ=255) aCGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATa > 8:85324/1‑149 (MQ=255) ctgGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGa > 7:51353/1‑148 (MQ=255) ctgGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGa > 2:165335/1‑148 (MQ=255) ctgGAAACTGTCAGCGGTCGTAACATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGACTGGGAGAGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGCACGGATACACCAGa > 4:8071/1‑148 (MQ=255) ggAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGGCGGATACACCAGAAAg > 4:31922/1‑149 (MQ=255) ggAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAg > 4:115612/1‑149 (MQ=255) ggTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGc > 4:100870/1‑149 (MQ=255) ggTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGc < 7:85324/149‑1 (MQ=255) tCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCgg < 5:154349/149‑1 (MQ=255) tATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑t < 2:98377/149‑1 (MQ=255) tATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑t < 3:115612/149‑1 (MQ=255) atcatcAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑tt > 2:72933/1‑149 (MQ=255) atcatcAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑tt > 6:198537/1‑149 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTgg < 5:196435/100‑1 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTgg > 6:196435/1‑100 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTc > 4:25985/1‑148 (MQ=255) atcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTc < 3:25985/148‑1 (MQ=255) tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTg < 4:216317/149‑1 (MQ=255) tcaACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGAACGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGC‑TTTCTg > 1:96677/1‑149 (MQ=255) caACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGGCGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTgg < 3:31922/149‑1 (MQ=255) caACGAAATCGACGGTATCTCGCGCGTTACCTACGACGGGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTgg < 7:198547/149‑1 (MQ=255) aCGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCAATCATGCGGGC‑TTTCTGGtg < 2:96677/149‑2 (MQ=255) aaaTCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCtt < 3:128859/149‑1 (MQ=255) aTCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTgt > 8:7617/1‑149 (MQ=255) tCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTgtt < 7:7617/149‑1 (MQ=255) gACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTgttc < 5:38727/148‑1 (MQ=255) cgcgTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCtt < 3:100870/149‑1 (MQ=255) cgTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGCTTTTCTGGTTCTTGTTCGTTCAGATTGCTTa < 1:165335/149‑1 (MQ=255) gTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTAtt < 2:99418/149‑1 (MQ=255) ttACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TATCTGGTTCTTGTTCGTTCAGATTGCTTAttc > 1:77866/1‑149 (MQ=255) ccTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTAttcttc < 2:125958/149‑1 (MQ=255) cTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TGTCTGGTTCTTGTTCGTTCAGATTGCTTAttcttat > 3:228941/1‑147 (MQ=255) acgacgTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTgg > 2:258308/1‑149 (MQ=255) acgTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCt > 6:282480/1‑135 (MQ=255) cgTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCttt < 1:258308/149‑1 (MQ=255) tCGAGCAAGCCGCCT‑GCCACTATGGAGTGGGAGTGATGGGGTAGCCAGCCCAGGCTGGA‑ACGCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTg < 3:8071/148‑1 (MQ=255) tCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTgg < 1:72933/149‑1 (MQ=255) gCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCaaa > 3:281648/1‑149 (MQ=255) aCTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTgg > 7:2717/1‑148 (MQ=255) aCTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGt < 8:51353/149‑1 (MQ=255) tCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTgg < 8:191525/144‑1 (MQ=255) tCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTgg > 7:191525/1‑144 (MQ=255) tGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGt < 3:60522/146‑1 (MQ=255) tGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGt > 4:60522/1‑146 (MQ=255) tGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGaaa < 5:198537/149‑1 (MQ=255) tgcgggtgccagccTGGGCTGGCTACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAag > 6:69377/8‑148 (MQ=255) cgggtgccagccTGGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtata < 3:29436/134‑1 (MQ=255) cgggtgccagccTGGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTtata > 4:29436/6‑139 (MQ=255) tAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTg < 8:2717/149‑1 (MQ=255) gtgccagccTGGGCTGGCTACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGt > 3:291778/3‑133 (MQ=255) gtgccagccTGGGCTGGCTACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGt < 4:291778/131‑1 (MQ=255) cagccCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGctcc > 4:43292/1‑149 (MQ=255) | CACCTGCCGTACGAGCTGCTGGAAACTGTCAGCGGTCGTATCATCAACGAAATCGACGGTATCTCGCGCGTTACCTACGACGTGTCGAGCAAGCCGCCT‑GCCACTATCGAGTGGGAGTGATCGGGTAGCCAGCCCAGGCTGGC‑ACCCGCAGGACGGATACACCAGAAAGCCCGCATGATTGCGGGC‑TTTCTGGTTCTTGTTCGTTCAGATTGCTTATTCTTCTGGCTTTCTGGCAAATAGCGCTTTCTGGTAGGCGTTATAGGAAAGCAGCCTGCTCC > NC_002947/1178576‑1178853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |