Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,184,813 C→T 7.5% G6G (GGC→GGT)  purL → phosphoribosylformylglycinamidine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,184,8130CT 7.5% 153.8 / 2.8 53G6G (GGC→GGT) purLphosphoribosylformylglycinamidine synthase
Reads supporting (aligned to +/- strand):  ref base C (27/22);  new base T (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGACCAGCAGCAGGTGCTG  >  NC_002947/1184669‑1184957
                                                                                                                                                |                                                                                                                                                
tGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTc                                                                                                                                              >  6:274755/1‑149 (MQ=255)
    aaCTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGc                                                                                                                                          >  7:93897/1‑149 (MQ=255)
                  tCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCCTTc                                                                                                                            <  2:110428/149‑1 (MQ=255)
                                   gCAGGCGTGGCTTATAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTc                                                                                                           <  3:192563/149‑1 (MQ=255)
                                    cAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCg                                                                                                          <  8:93897/149‑1 (MQ=255)
                                       gCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTTCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGc                                                                                                       <  8:179098/149‑1 (MQ=255)
                                          tGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCt                                                                                                                                       >  1:241249/1‑114 (MQ=255)
                                          tGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCt                                                                                                                                       <  2:241249/114‑1 (MQ=255)
                                             cTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACt                                                                                                   >  3:122411/1‑147 (MQ=255)
                                                   gCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAg                                                                                            >  1:171315/1‑148 (MQ=255)
                                                     aGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACTATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGaaa                                                                                         <  7:136020/149‑1 (MQ=255)
                                                             aaaGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGc                                                                                 <  5:274755/149‑1 (MQ=255)
                                                             aaaGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGc                                                                                 >  4:183497/1‑149 (MQ=255)
                                                                 ccATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGt                                                                              >  4:31414/1‑148 (MQ=255)
                                                                 caaTCGAATTAGGCTAGAATGCACGGCCTCTAAGCACGCCCCTTCCTGAGCCTGTCCCGACGATGTTGATCCTGCGCCTCGCTCCTGACCTTTCTCCCTTGCTCCACGGTAAAATACTCGAGCACCTGAGCCAGAAGGTCGCCGCTGt                                                                              >  2:60132/3‑148 (MQ=255)
                                                                  cATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGtt                                                                             >  4:182990/1‑148 (MQ=255)
                                                                   aTCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTAc                                                                           >  3:42822/1‑149 (MQ=255)
                                                                     cGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCt                                                                                                                                               <  4:75610/79‑1 (MQ=255)
                                                                               tAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGc                                                               >  7:249474/1‑149 (MQ=255)
                                                                                      cACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAAtt                                                         <  2:171315/148‑1 (MQ=255)
                                                                                       aCGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGAAGATGTTGATCCTGCGCGGTGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTACCCGCTGTTACTGGTTTGTATGCCGAATTTg                                                       >  5:220952/1‑149 (MQ=255)
                                                                                         ggCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTAc                                                                           >  2:204631/1‑127 (MQ=255)
                                                                                         ggCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTAc                                                                           <  1:204631/127‑1 (MQ=255)
                                                                                         ggCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGcc                                                     >  7:28458/1‑149 (MQ=255)
                                                                                             tctAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACt                                                 >  2:306037/1‑149 (MQ=255)
                                                                                                 aGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGc                                             <  3:182990/149‑1 (MQ=255)
                                                                                                 aGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGc                                             >  2:1517/1‑149 (MQ=255)
                                                                                                    acacCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGTGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGa                                          >  4:207986/1‑149 (MQ=255)
                                                                                                     cacCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGAt                                         <  4:122411/149‑1 (MQ=255)
                                                                                                        cccTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTc                                      >  3:88695/1‑149 (MQ=255)
                                                                                                        cccTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTc                                      >  8:217334/1‑149 (MQ=255)
                                                                                                        cccTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTc                                      >  3:2155/1‑149 (MQ=255)
                                                                                                         ccTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTc                                      >  6:238021/1‑148 (MQ=255)
                                                                                                          cTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGa                                    >  7:198082/1‑149 (MQ=255)
                                                                                                             ccTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGTGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGAc                                   <  3:207986/147‑1 (MQ=255)
                                                                                                              cTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGc                                <  1:1517/149‑1 (MQ=255)
                                                                                                              cTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGc                                <  7:217334/149‑1 (MQ=255)
                                                                                                                gAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGa                              >  5:22317/1‑149 (MQ=255)
                                                                                                                 aGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCg                               <  3:31414/147‑1 (MQ=255)
                                                                                                                 aGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAg                             <  4:42822/149‑1 (MQ=255)
                                                                                                                     tGTCCCGACGATGTTGATCCTGCGCGGTGCTCCTGCCCTTTCTGCCTTTCGCCACGTTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGa                         <  6:220952/149‑1 (MQ=255)
                                                                                                                       tCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGAcc                       <  4:88695/149‑1 (MQ=255)
                                                                                                                         ccGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGAccgc                     >  2:63860/1‑149 (MQ=255)
                                                                                                                          cgacgaTGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGAccgcc                    >  5:304986/1‑149 (MQ=255)
                                                                                                                             cgaTGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGAc                 <  8:198082/149‑1 (MQ=255)
                                                                                                                                 gTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGACcag              >  3:46003/1‑148 (MQ=255)
                                                                                                                                  ttGATCCTGCGCTGCGGTGCTGCGCTTTCTGCCTTTGGCAACGGTAAATTACTCGAGGAACTGAGCCAGAAAGTCCCCGGTGTTAGTGGTTTGTATGCCGATTTTGCCCACTTCGCCGATGTCGACGTCTAGCTGACCGCCGACcagc             <  1:60132/148‑1 (MQ=255)
                                                                                                                                  ttGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGACcagc             <  4:46003/148‑1 (MQ=255)
                                                                                                                                    gATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGACcagcag           >  2:194361/1‑148 (MQ=255)
                                                                                                                                     aTCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAg                             >  6:240111/1‑129 (MQ=255)
                                                                                                                                     aTCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAg                             <  5:240111/129‑1 (MQ=255)
                                                                                                                                      tCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGTCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGACcagcagcag        >  6:198225/1‑149 (MQ=255)
                                                                                                                                        cTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGACCAGCAGCAGGt      <  6:22317/149‑1 (MQ=255)
                                                                                                                                            gcggcgCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCCCCGATGTCGACGGCGAGCTGACCGCCGACCAGCAGCAGGTGCTg  >  6:21843/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGAAAACTGACCCCGAAGTCCGGTTCCGCCGCCCGGCAGGCGTGGCTTAGAGCAGGTGCCGAAAGCCATCGAATTAGGCTAGAATGCACGGCCTCTAAGCACACCCCTTCCTGAGGCTGTCCCGACGATGTTGATCCTGCGCGGCGCTCCTGCCCTTTCTGCCTTTCGCCACGGTAAATTACTCGAGCAACTGAGCCAGAAAGTCCCCGCTGTTACTGGTTTGTATGCCGAATTTGCCCACTTCGCCGATGTCGACGGCGAGCTGACCGCCGACCAGCAGCAGGTGCTG  >  NC_002947/1184669‑1184957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑