Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,193,929 T→A 7.0% L23* (TTG→TAG) ‡ uxpA → lipoprotein UxpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,193,9290TA 7.0% 167.8 / 2.8 57L23* (TTG→TAG) ‡uxpAlipoprotein UxpA
Reads supporting (aligned to +/- strand):  ref base T (27/26);  new base A (2/2);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

CTGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCAGC  >  NC_002947/1193789‑1194074
                                                                                                                                            |                                                                                                                                                 
cTGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCgc                                                                                                                                           <  6:264846/149‑1 (MQ=255)
cTGTCGGATTTCTGCCATCAAACTGTAACGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCAAGGCACCga                                                                                                                                           <  5:233377/149‑2 (MQ=255)
 tGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCgct                                                                                                                                          >  3:36580/1‑149 (MQ=255)
   tCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCgctgc                                                                                                                                        >  7:248271/1‑149 (MQ=255)
      gATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCg                                                                                                                                     >  4:278372/1‑149 (MQ=255)
           cTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCg                                                                                                                                            <  6:109371/137‑1 (MQ=255)
           cTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCg                                                                                                                                            >  5:109371/1‑137 (MQ=255)
               cATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGca                                                                                                                            >  3:245701/1‑149 (MQ=255)
                aTCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGca                                                                                                                            >  4:100489/1‑148 (MQ=255)
                 tCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGcaca                                                                                                                          <  1:61071/149‑1 (MQ=255)
                 tCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGca                                                                                                                            >  5:263778/1‑147 (MQ=255)
                    aaCTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGcaca                                                                                                                          <  1:229363/146‑1 (MQ=255)
                    aaCTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGcaca                                                                                                                          >  2:229363/1‑146 (MQ=255)
                       tGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTc                                                                                                                    >  2:93418/1‑149 (MQ=255)
                       tGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCAAGGCACCgat                                                                                                                                          <  8:97725/127‑3 (MQ=255)
                       tGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCAAGGCACCgat                                                                                                                                          >  7:97725/1‑125 (MQ=255)
                         tAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTcc                                                                                                                   <  4:33006/148‑1 (MQ=255)
                         tAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTc                                                                                                                    <  3:100489/147‑1 (MQ=255)
                             gCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAggg                                                                                                              >  6:212308/1‑149 (MQ=255)
                              ccAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAggg                                                                                                              <  5:112407/148‑1 (MQ=255)
                              ccAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAggg                                                                                                              <  4:36580/148‑1 (MQ=255)
                                      gcAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACGGCCTTCCCAGGGCAGCAGggc                                                                                                     >  8:211555/1‑149 (MQ=255)
                                       cAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGggcg                                                                                                    <  3:278372/149‑1 (MQ=255)
                                            tCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAAt                                                                                               <  6:195811/149‑1 (MQ=255)
                                                     cTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGcac                                                                                                                           <  7:129329/112‑1 (MQ=255)
                                                     cTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGcac                                                                                                                           >  8:129329/1‑112 (MQ=255)
                                                        aaTGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGAc                                                                                   <  5:167650/149‑1 (MQ=255)
                                                         aTGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACt                                                                                  >  3:200435/1‑149 (MQ=255)
                                                              cGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTg                                                                             <  6:263778/149‑1 (MQ=255)
                                                                tGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTa                                                                           <  8:177261/149‑1 (MQ=255)
                                                                  gTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCTGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTa                                                                           <  1:36289/147‑1 (MQ=255)
                                                                  gTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTa                                                                           >  2:36289/1‑147 (MQ=255)
                                                                      aGGATGGCGGATGCGATGGCGAGCAGTGTGCGCAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGc                                                                     >  7:253040/1‑149 (MQ=255)
                                                                              ggATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGGTAccc                                                             <  1:93418/149‑1 (MQ=255)
                                                                                  gCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGa                                                         >  3:163992/1‑149 (MQ=255)
                                                                                          gAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGc                                                  >  1:180683/1‑148 (MQ=255)
                                                                                             cAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTgat                                                                              >  7:34672/1‑115 (MQ=255)
                                                                                             cAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCt                                                                              <  8:34672/117‑1 (MQ=255)
                                                                                               gtgtGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAg                                                                                         <  6:82229/104‑1 (MQ=255)
                                                                                                    cgcaGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGcc                                        <  8:253040/146‑1 (MQ=255)
                                                                                                       aGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGc                                    >  2:83577/1‑149 (MQ=255)
                                                                                                             gAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCt                                            <  1:137902/135‑1 (MQ=255)
                                                                                                             gAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCt                                            >  2:137902/1‑135 (MQ=255)
                                                                                                               aGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTAc                            <  5:212308/149‑1 (MQ=255)
                                                                                                                           gATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTg                                                                             <  6:207489/88‑1 (MQ=255)
                                                                                                                           gATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTg                                                                             >  5:207489/1‑88 (MQ=255)
                                                                                                                               gcgaTCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCCGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGGCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTggg            <  2:216007/146‑1 (MQ=255)
                                                                                                                                 tATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACGGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGc          <  7:211555/149‑1 (MQ=255)
                                                                                                                                 tATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGc          >  1:142277/1‑149 (MQ=255)
                                                                                                                                   gcGGTGCCAAGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCg        >  1:149271/2‑149 (MQ=255)
                                                                                                                                    cGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCgg       >  2:296471/1‑149 (MQ=255)
                                                                                                                                    cGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCgg       >  2:41932/1‑149 (MQ=255)
                                                                                                                                       tGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCa    <  4:200435/149‑1 (MQ=255)
                                                                                                                                        gCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCAg   >  2:24591/1‑149 (MQ=255)
                                                                                                                                          cTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCggtg                                <  6:213555/118‑1 (MQ=255)
                                                                                                                                          cTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCggtg                                >  5:213555/1‑118 (MQ=255)
                                                                                                                                          cTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACATCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCAGc  >  5:5534/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                                 
CTGTCGGATTTCTGCCATCAAACTGTAATGCCAACGCCGCAACATCCGGGTCCCTGAATGGACGTGGTTAAGGATGGCGGATGCGATGGCGAGCAGTGTGCACAGGCGCGAAGTGATCGGCTGGATGGGTATCGGTGCCTTGGCACCGCTGCTCGGCGCCTGCACAGCCTTCCCAGGGCAGCAGGGCGGCAATGCCCAGCTCGACTTGCTGTACGTCGCCGATACCCTCGATGCCCGCCAGCCTGGCCAGGCGGTGGTACCGGCTACCCGGTTGGGGCCGGTCAGC  >  NC_002947/1193789‑1194074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑