Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,206,693 G→C 6.5% L770V (CTG→GTG)  PP_1058 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,206,6930GC 6.5% 197.2 / 4.0 62L770V (CTG→GTG) PP_1058hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base C (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGGATGCGGCTGTCACGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTGGGCACGCACGAACGCGTTGAAG  >  NC_002947/1206548‑1206841
                                                                                                                                                 |                                                                                                                                                    
cGGATGCGGCTGTCACGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCgg                                                                                                                                                   >  2:194307/1‑149 (MQ=255)
      cGGCTGTCACGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGa                                                                                                                                              <  1:194307/148‑1 (MQ=255)
         cTGTCACGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCtt                                                                                                                                          >  1:102975/1‑149 (MQ=255)
            tCACGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGc                                                                                                                                       <  1:196762/149‑1 (MQ=255)
            tCACGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGc                                                                                                                                       <  5:119292/149‑1 (MQ=255)
              aCGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCg                                                                                                                                      <  2:102975/148‑1 (MQ=255)
              aCGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCg                                                                                                                                      <  6:295535/148‑1 (MQ=255)
                gggCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGAt                                                                                                                                    <  3:70881/148‑1 (MQ=255)
                gggCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGAt                                                                                                                                    >  4:70881/1‑148 (MQ=255)
                  gcgcCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGt                                                                                                                                  >  4:73829/1‑148 (MQ=255)
                   cgcCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTaa                                                                                                                                <  8:263654/149‑1 (MQ=255)
                      ccTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGt                                                                                                                                  >  4:265432/1‑144 (MQ=255)
                      ccTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGt                                                                                                                                  <  3:265432/144‑1 (MQ=255)
                        tggtggCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTg                                                                                                                           >  6:95346/1‑149 (MQ=255)
                               ggcTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTAGATCTCGGTTTAATCAGAATGTATAAAAAAAACCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCggg                                                                                                                     >  8:44180/1‑148 (MQ=255)
                                  tctTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAgg                                                                                                                 >  7:120749/1‑149 (MQ=255)
                                      gAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCg                                                                                                              >  2:244582/1‑148 (MQ=255)
                                       aaCAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTa                                                                                                            >  8:231762/1‑149 (MQ=255)
                                       aaCAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGGCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTa                                                                                                            <  8:32556/149‑1 (MQ=255)
                                          agccagccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGTTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACtt                                                                                                         <  4:275066/149‑1 (MQ=255)
                                            ccagccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGc                                                                                                       <  7:44180/149‑1 (MQ=255)
                                              agccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCg                                                                                                     <  3:276725/149‑1 (MQ=255)
                                               gccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCgg                                                                                                    >  5:123114/1‑149 (MQ=255)
                                               gccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCgg                                                                                                    <  1:244582/149‑1 (MQ=255)
                                               gccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCACCCGCTGGACCtgt                                                                                                                                         >  4:117231/1‑110 (MQ=255)
                                               gccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCACCCGCTGGACCtgt                                                                                                                                         <  3:117231/112‑3 (MQ=255)
                                                ccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTg                                                                                                                           >  2:209352/1‑125 (MQ=255)
                                                ccGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTg                                                                                                                           <  1:209352/125‑1 (MQ=255)
                                                   tataCCTCCTGGATTTCGAAAAGACTGGCGTAGATCATCTCGGTCAGTGTCGACCTCGGGTGATTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTCGCGATGTACCCCTCGTAGGCCAGGTCGTACAAGCCGGGGcc                                                                                                >  2:244175/1‑149 (MQ=255)
                                                       cctcctGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTaa                                                                                            <  1:266708/149‑1 (MQ=255)
                                                        ctcctGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAg                                                                                           <  7:231762/149‑1 (MQ=255)
                                                           ctGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCt                                                                                         <  6:123114/148‑1 (MQ=255)
                                                                 ttCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGt                                                                                                             <  1:248676/122‑1 (MQ=255)
                                                                 ttCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACa                                                                                  <  3:73829/149‑1 (MQ=255)
                                                                 ttCGAAACGACAGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGt                                                                                                             >  2:248676/1‑122 (MQ=255)
                                                                      aaCGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCg                                                                             <  5:95346/149‑1 (MQ=255)
                                                                          aCTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTaa                                                                                            >  6:260673/1‑130 (MQ=255)
                                                                          aCTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTaa                                                                                            <  5:260673/130‑1 (MQ=255)
                                                                                     tcatcTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGACAAGAAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCg                                                              >  8:180068/1‑149 (MQ=255)
                                                                                        tctcGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGt                                                            >  7:31619/1‑148 (MQ=255)
                                                                                          tgggTCAGTGTCGATCTCGTGTTCTGCAGCAGGTAGCCAACCAGCCACAGTTCCAGTGGGTGGACCTTGGCGATGTAACCCTGGTCCGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAg                                                         <  1:244175/147‑1 (MQ=255)
                                                                                              tCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTg                                                     >  5:234627/1‑149 (MQ=255)
                                                                                                 gtgtCGAGCTCGGGTTCTTCAGCATGTAGACAATCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGcc                                                   >  2:19985/1‑148 (MQ=255)
                                                                                                  tgtCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGcc                                                   >  6:292438/1‑147 (MQ=255)
                                                                                                      gAGCTCGGGTTCTTCAGCAGGTAGCCAAACAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCAc                                             >  3:238638/1‑149 (MQ=255)
                                                                                                              gTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTACAGCCGGTGGACCTTGGCCATGTAACCCTGGTCGGCCAGGTAGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGt                                     >  8:85612/1‑149 (MQ=255)
                                                                                                               ttcttcAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGt                                     >  5:111927/1‑148 (MQ=255)
                                                                                                                    cagcagGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGc                                                                   <  6:23739/113‑1 (MQ=255)
                                                                                                                    cagcagGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGc                                                                   >  5:23739/1‑113 (MQ=255)
                                                                                                                       cagGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCtt                              >  5:182923/1‑147 (MQ=255)
                                                                                                                          gTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGAcc                                         <  7:219108/133‑1 (MQ=255)
                                                                                                                          gTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGAcc                                         >  8:219108/1‑133 (MQ=255)
                                                                                                                           tAGCCAAACAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTg                        <  4:238638/149‑1 (MQ=255)
                                                                                                                              ccAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTGGgc                     <  4:111042/149‑1 (MQ=255)
                                                                                                                                        acaGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTGGGCACGCACGAAc           >  3:52303/1‑149 (MQ=255)
                                                                                                                                         aaGGTCCACCCGCTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCg                                                                             <  7:133520/81‑1 (MQ=255)
                                                                                                                                         aaGGTCCACCCGCTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCg                                                                             >  8:133520/2‑82 (MQ=255)
                                                                                                                                            ggccAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTGGGCACGCACGAACGCGt       <  8:120749/147‑1 (MQ=255)
                                                                                                                                             tCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTGGGCACGCACGAACGCGtt      <  6:111927/149‑1 (MQ=255)
                                                                                                                                              ccAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACg                                                                     >  8:291633/1‑85 (MQ=255)
                                                                                                                                              ccAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACg                                                                     <  7:291633/85‑1 (MQ=255)
                                                                                                                                               cAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAg                                                <  8:281347/105‑1 (MQ=255)
                                                                                                                                                 gCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTGGGCACGCACGAACGCGTTGAAg  <  5:292438/149‑1 (MQ=255)
                                                                                                                                                 gCGGGTGGACCGTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCCCGACCTTGTCACCCTTGAGGTGGCCACGCACGCACGCGTTGAAg  >  4:217516/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CGGATGCGGCTGTCACGGGCGCCCTGGTGGCGGCTCTTGAACAGCCAGCCGTATACCTCCTGGCGTTCGAAACGACTGGCGTTGATCATCTCGGTCAGTGTCGAGCTCGGGTTCTTCAGCAGGTAGCCAAGCAGCCACAGGTCCAGCGGGTGGACCTTGGCGATGTAACCCTGGTCGGGCAGGTCGTACTTGCCGGGGCCGTAAGCTACGTACATCTCGGACAGACGGCCATCGGTCAGTTTGCCCAGCACGACCTTGTCACCCTTGAGGTGGGCACGCACGAACGCGTTGAAG  >  NC_002947/1206548‑1206841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑