Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	155,543	G→T	6.0%	intergenic (‑248/+35)	yjbB ← / ← ygdQ	Na+/Pi‑cotransporter/transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	155,543	0	G	T	6.0%	212.1 / 3.0	67	intergenic (‑248/+35)	yjbB/ygdQ	Na+/Pi‑cotransporter/transport protein
Reads supporting (aligned to +/- strand):  ref base G (31/32);  new base T (2/2);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CAGCTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGG  >  NC_002947/155395‑155690                                                                                                                                                     |                                                                                                                                                    cAGCTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCAGAGAAAAAAGGCATTAAAAAGGGCCCTg                                                                                                                                                     <  2:291287/149‑1 (MQ=255) cAGCTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTg                                                                                                                                                     >  4:42434/1‑149 (MQ=255) cAGCTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCA‑GAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGc                                                                                                                                                    >  3:172991/1‑149 (MQ=255)            aGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCt                                                                                                                                          >  3:121147/1‑149 (MQ=255)              cTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttg                                                                                                                                        <  2:46722/149‑1 (MQ=255)                 cGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttgtt                                                                                                                                      <  3:110867/148‑1 (MQ=255)                 cGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCA‑GAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCttgtt                                                                                                                                      <  4:172991/147‑1 (MQ=255)                  gCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTTCGCAGGGCCCtttttaa                                                                                                                                    <  1:58897/149‑3 (MQ=255)                      ggCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCtttttaatgcc                                                                                                                                >  2:199884/1‑143 (MQ=255)                         aGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCAc                                                                                                                             >  3:292968/1‑149 (MQ=255)                          gTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACt                                                                                                                            <  8:26555/149‑1 (MQ=255)                           tCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCTTTAAAAAGGGCCCTGCGa                                                                                                                                                  >  5:264071/1‑126 (MQ=255)                           tCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGa                                                                                                                                                  <  6:264071/126‑1 (MQ=255)                               ttatttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCtt                                                                                                                       >  8:155680/1‑149 (MQ=255)                                tatttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCAACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTg                                                                                                                      >  7:289590/1‑149 (MQ=255)                                 atttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTg                                                                                                                      <  6:128008/148‑1 (MQ=255)                                  tttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTc                                                                                                                          >  7:87798/1‑143 (MQ=255)                                  tttGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTc                                                                                                                          <  8:87798/143‑1 (MQ=255)                                       aaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCATGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTa                                                                                                                <  5:253424/148‑1 (MQ=255)                                       aaCAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAt                                                                                                               >  4:56700/1‑149 (MQ=255)                                          aGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGa                                                                                                            >  5:130771/1‑149 (MQ=255)                                               ttGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAtt                                                                                                              <  2:69397/142‑1 (MQ=255)                                               ttGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTAtt                                                                                                              >  1:69397/1‑142 (MQ=255)                                                 gAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCgg                                                                                                       <  6:35821/147‑1 (MQ=255)                                                   aaCGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGTAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATg                                                                                                   <  6:147424/149‑1 (MQ=255)                                                     cGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTc                                                                                                 <  6:130771/149‑1 (MQ=255)                                                       aaTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAATGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCt                                                                                               >  6:119429/1‑149 (MQ=255)                                                       aaTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATg                                                                                                   <  2:27449/145‑1 (MQ=255)                                                       aaTGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATg                                                                                                   >  1:27449/1‑145 (MQ=255)                                                             tGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTgcg                                                                                           >  6:104272/1‑147 (MQ=255)                                                               cccGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGt                                                                                       <  4:136104/149‑1 (MQ=255)                                                                     ggggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCAc                                                                                  <  3:42434/148‑1 (MQ=255)                                                                      gggCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCg                                                                                >  2:176802/1‑149 (MQ=255)                                                                        gctgctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTg                                                                                                             >  4:198542/1‑118 (MQ=255)                                                                        gctgctTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTg                                                                                                             <  3:198542/118‑1 (MQ=255)                                                                                gcgGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGAt                                                                       >  7:246178/1‑148 (MQ=255)                                                                                    gCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTg                                                                  <  3:56700/149‑1 (MQ=255)                                                                                     cAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCa                                                                           >  5:116042/1‑139 (MQ=255)                                                                                     cAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCa                                                                           <  6:116042/139‑1 (MQ=255)                                                                                      aaGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGcc                                                                <  8:171712/149‑1 (MQ=255)                                                                                      aaGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGc                                                                 >  1:268397/1‑148 (MQ=255)                                                                                        gCCACAACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCAt                                                              >  2:203460/1‑149 (MQ=255)                                                                                             cacaGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTgcg                                                                                           <  1:138936/115‑1 (MQ=255)                                                                                             cacaGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTgcg                                                                                           >  2:138936/1‑115 (MQ=255)                                                                                                aGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                      <  8:181293/149‑1 (MQ=255)                                                                                                aGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCgg                                                      <  8:289590/149‑1 (MQ=255)                                                                                                    aCAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCa                                                  >  5:31282/1‑149 (MQ=255)                                                                                                         gcgcAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCg                                             >  4:285984/1‑149 (MQ=255)                                                                                                         gcgcAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCg                                             <  8:246178/149‑1 (MQ=255)                                                                                                           gcAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGt                                           >  4:280827/1‑149 (MQ=255)                                                                                                                        cacaGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATg                              <  2:268397/149‑1 (MQ=255)                                                                                                                          caGAAAAAAGGCATAAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCATTGGCCTTAATGACCCGGAATGACCTTGCGCAGATACACCGGTTCATGATACTTGAAATCCATGCGGGACATGGCGGTGCCCATGCGCATGtt                            >  1:271322/1‑149 (MQ=255)                                                                                                                            gAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCtt                                                                   <  5:252841/108‑1 (MQ=255)                                                                                                                            gAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCtt                                                                   >  6:252841/1‑108 (MQ=255)                                                                                                                             aaaaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                         <  3:280827/149‑1 (MQ=255)                                                                                                                              aaaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                         <  1:203460/148‑1 (MQ=255)                                                                                                                              aaaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGAt                         <  1:176802/148‑1 (MQ=255)                                                                                                                               aaaaGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATgg                       >  5:286988/1‑149 (MQ=255)                                                                                                                                          aaaaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGc             <  7:155680/148‑1 (MQ=255)                                                                                                                                           caaGGGCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCa           >  8:118511/2‑149 (MQ=255)                                                                                                                                            aaGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAg          >  4:270055/1‑149 (MQ=255)                                                                                                                                              gggCCCTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGc         >  5:204156/1‑148 (MQ=255)                                                                                                                                                gCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCgag      <  6:31282/149‑1 (MQ=255)                                                                                                                                                  ccTTCGCAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGaa    <  6:204156/149‑1 (MQ=255)                                                                                                                                                  ccTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCgag      >  6:59539/1‑147 (MQ=255)                                                                                                                                                   cTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAg   <  3:270055/149‑1 (MQ=255)                                                                                                                                                    tGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCga       <  3:78215/144‑1 (MQ=255)                                                                                                                                                    tGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCga       >  4:78215/1‑144 (MQ=255)                                                                                                                                                    tGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAgg  >  4:264687/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CAGCTGTCGCCAGCTTCGCATGGCAGTCATTTATTTGAAACAGATCTTGAAACGAATGACTGCCCGTGGGGGCTGCTTCGCGGGCAAGCCACCACAGTGACAACGCGCAGGAAAAAACGCACAGAAAAAAGGCATTAAAAAGGGCCCTGCGAAGGGCCCTTGTTGTGCTGCACTTCCTTGGCCTTATTGACCCGGAATGTCCTTGCGCAGTTTCACCGGTTCATGATCCTTGCCATCCTTGCGGGCCATGGCGGTGCGCATGCGGATGTTGATGGCTTCCACCGCCAGCGAGAAGG  >  NC_002947/155395‑155690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑