Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,239,103 A→C 9.4% intergenic (‑78/‑297) argF ← / → PP_1080 ornithine carbamoyltransferase/molybdopterin‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,239,1030AC 9.4% 104.1 / 3.0 41intergenic (‑78/‑297)argF/PP_1080ornithine carbamoyltransferase/molybdopterin‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (21/16);  new base C (2/2);  total (23/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

TGGCGGATCACCCCGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTA  >  NC_002947/1238958‑1239247
                                                                                                                                                 |                                                                                                                                                
tGGCGGATCACCCCGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGcc                                                                                                                                               >  5:39717/1‑149 (MQ=255)
           cccGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCgg                                                                                                                                    >  8:78085/1‑149 (MQ=255)
             cGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGAc                                                                                                                                  <  7:78085/149‑1 (MQ=255)
                   aTTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGaaa                                                                                                                              >  8:115854/1‑147 (MQ=255)
                    ttCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAg                                                                                                                           <  2:75407/149‑1 (MQ=255)
                              gtgAAGTCCAGACGGGAGAGAAAGTGCCTCGCGCTAATTAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGgcgc                                                                                                                 <  4:207646/149‑1 (MQ=255)
                                   gTCCATCAGTGAGAGAAAGTGCCTAGCGTTCATGAGTGAATACCTTATCTGCAACGGTATGGAGGGCGACGGTTTGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGATATAg                                                                                                                           <  2:210680/134‑1 (MQ=255)
                                   gTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTAACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGTTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAg                                                                                                                           >  1:210680/1‑134 (MQ=255)
                                       agcagGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGCGCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACtat                                                                                                         >  7:101079/1‑148 (MQ=255)
                                                               cTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAATCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAgg                                                                                >  2:264345/1‑149 (MQ=255)
                                                                  aTGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAggggg                                                                             >  5:173850/1‑149 (MQ=255)
                                                                    gAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGACGCCTGAAGCAAAgtgggg                                                                            >  8:261909/1‑148 (MQ=255)
                                                                     aGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGa                                                                           >  6:169079/1‑148 (MQ=255)
                                                                         aCTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAgc                                                                      >  3:139673/1‑149 (MQ=255)
                                                                               ttATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCg                                                                                             >  6:230377/1‑120 (MQ=255)
                                                                               ttATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCg                                                                                             <  5:230377/120‑1 (MQ=255)
                                                                               ttATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAACAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGt                                                                 >  5:174137/1‑148 (MQ=255)
                                                                                      cAACGGTATGCAGGGCGACCGTATGGGTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAtt                                                             <  5:306264/145‑1 (MQ=255)
                                                                                          ggTATGGATGTCGACGGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGGCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagg                                                      <  8:212740/148‑1 (MQ=255)
                                                                                           gTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagg                                                      >  7:212740/1‑147 (MQ=255)
                                                                                                cAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttg                                                >  5:259731/1‑148 (MQ=255)
                                                                                                   gTCGACCGTATGGTTTTTTTTGACAACGGGGGTGCCATGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCATTAGTATAAGTAAATGTCGCGTGAAGCCATGGGGGGAAATGCGGCTTGAttgcaaaggttgttgca                                              >  3:193947/1‑147 (MQ=255)
                                                                                                       aCCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaagggt                                        <  4:139673/149‑1 (MQ=255)
                                                                                                               ggtttttttGACAACGGGGGTCACCTGCGGCGTACCCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTAc                                <  6:39717/147‑1 (MQ=255)
                                                                                                               gTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgt                                                  <  1:303262/131‑1 (MQ=255)
                                                                                                               gTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgt                                                  >  2:303262/1‑131 (MQ=255)
                                                                                                               gTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTAc                                <  1:264345/149‑1 (MQ=255)
                                                                                                                ttttttttGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaagg                                          <  4:219232/138‑1 (MQ=255)
                                                                                                                ttttttttGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgcaaagg                                          >  3:219232/1‑138 (MQ=255)
                                                                                                                         aCAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGTGGAAAAGCGGCGTGATTGCAAAGCGTGTTGCAAAGGGTGTCTTTACGCGTTGcccc                      >  3:267926/1‑149 (MQ=255)
                                                                                                                             cGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGc                  >  1:286852/1‑149 (MQ=255)
                                                                                                                              gggggTGCCCGGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAACTGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGGGGTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCt                 <  5:166155/149‑1 (MQ=255)
                                                                                                                                      cgtGCGGCTTACGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgc                                               >  5:36476/3‑111 (MQ=255)
                                                                                                                                      cgtGCGGCTTACGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGAttgcaaagggtgttgc                                               <  6:36476/109‑1 (MQ=255)
                                                                                                                                      cgtGCGGCTTACGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTa         >  3:193957/3‑149 (MQ=255)
                                                                                                                                       cTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAt        >  6:298517/1‑149 (MQ=255)
                                                                                                                                        tGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGtt              <  4:202419/142‑1 (MQ=255)
                                                                                                                                        tGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGtt              >  3:202419/1‑142 (MQ=255)
                                                                                                                                         gcggcgTAAGCCGCAGGGGGCGGACGAAATAGGGAAATGCGCCATACTATAAGTAAATGTCGCCTTAATCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTAttt      <  6:259731/149‑1 (MQ=255)
                                                                                                                                             cgTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTa  <  5:169079/149‑1 (MQ=255)
                                                                                                                                             cgTAAGCCGCACGGGGCGGACGAAACAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTa  <  6:174137/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGCGGATCACCCCGAGCAATTCGTCGGTGGTGAAGTCCAGCAGGGAGAGAAAGTGCCTAGCGCTCATGAGTGACTACCTTATCTGCAACGGTATGCAGGTCGACCGTATGGTTTTTTTTGACAACGGGGGTGACCTGCGGCGTAAGCCGCACGGGGCGGACGAAATAGGGAAAGGCGCAATACTATAAGTAAATGTCGCCTGAAGCCAAGGGGGGAAAAGCGGCGTGATTGCAAAGGGTGTTGCAAAGGGTGTCTTTACCCGTTGGCCCGTGCTGTTCTTTATTTGCTA  >  NC_002947/1238958‑1239247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑