Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,243,725 G→A 7.3% intergenic (+14/+85) tsaA → / ← rnt peroxiredoxin/ribonuclease T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,243,7250GA 7.3% 238.3 / 5.8 82intergenic (+14/+85)tsaA/rntperoxiredoxin/ribonuclease T
Reads supporting (aligned to +/- strand):  ref base G (40/36);  new base A (2/4);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

GGTTGACGCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCG  >  NC_002947/1243579‑1243864
                                                                                                                                                  |                                                                                                                                              
ggTTGACGCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGa                                                                                                                                               >  3:37653/1‑148 (MQ=255)
  ttGACGCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATaaaa                                                                                                                                             >  7:166575/1‑148 (MQ=255)
      cGCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAAcc                                                                                                                                        <  5:313250/149‑1 (MQ=255)
      cGCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAAcc                                                                                                                                        <  7:131166/149‑1 (MQ=255)
       gCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCg                                                                                                                                       >  5:312899/1‑149 (MQ=255)
       gCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCg                                                                                                                                       <  6:312899/149‑1 (MQ=255)
       gCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCg                                                                                                                                       >  8:155429/1‑149 (MQ=255)
       gCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCg                                                                                                                                       >  7:29526/1‑149 (MQ=255)
       gCTCCGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCg                                                                                                                                       >  2:53988/1‑149 (MQ=255)
        ctctGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCgg                                                                                                                                      <  7:155429/149‑1 (MQ=255)
             cAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTc                                                                                                                                 <  8:10652/149‑1 (MQ=255)
                  ccACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTgg                                                                                                                             <  7:112569/148‑1 (MQ=255)
                    aCGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCg                                                                                                                          >  5:130047/1‑149 (MQ=255)
                        agagCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGttt                                                                                                                      <  3:278368/149‑1 (MQ=255)
                         gagCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGtttt                                                                                                                     <  8:166575/149‑1 (MQ=255)
                          agCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGttttt                                                                                                                    >  7:116026/1‑149 (MQ=255)
                              cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGtttttagat                                                                                                                >  5:162510/1‑145 (MQ=255)
                              cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGtttt                                                                                                                     <  6:162510/144‑1 (MQ=255)
                              cGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTAt                                                                                                                <  8:23049/149‑1 (MQ=255)
                               ggCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATg                                                                                                               <  6:130047/149‑1 (MQ=255)
                               ggCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCATg                                                                                                               <  5:262059/149‑1 (MQ=38)
                               ggCGAAGTGTCCCCTGCCAACTCGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGAGCCCGGTTTTTTTATg                                                                                                               >  8:287032/1‑149 (MQ=255)
                                       gtgCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCtg                                                                                                       >  4:209825/1‑149 (MQ=255)
                                          cccTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGa                                                                                                                                               >  6:119653/1‑106 (MQ=255)
                                          cccTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGa                                                                                                                                               <  5:119653/106‑1 (MQ=255)
                                           ccTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGcc                                                                                                   >  8:41427/1‑149 (MQ=255)
                                               ccAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGc                                                                                                >  3:243066/1‑148 (MQ=255)
                                                 aaCTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAAc                                                                                                                                         <  2:124327/105‑1 (MQ=255)
                                                 aaCTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAAc                                                                                                                                         >  1:124327/1‑105 (MQ=255)
                                                    tGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTc                                                                                        >  4:24197/1‑149 (MQ=255)
                                                            aGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGgcgc                                                                                >  8:95374/1‑149 (MQ=255)
                                                                gACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGccc                                                                            >  7:162949/1‑149 (MQ=255)
                                                                gACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGccc                                                                            >  1:297134/1‑149 (MQ=255)
                                                                  cAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCcgc                                                                          <  8:29526/149‑1 (MQ=255)
                                                                  cAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCcgc                                                                          <  8:162949/149‑1 (MQ=255)
                                                                     gggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCATGc                                                                                                              <  4:65354/112‑1 (MQ=37)
                                                                     gggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCATGc                                                                                                              <  4:69525/112‑1 (MQ=37)
                                                                     gggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCATGc                                                                                                              >  3:69525/1‑112 (MQ=37)
                                                                     gggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATAAAAAAACCGGCCACGAAGGCCGGTTTTTTCATGc                                                                                                              >  3:65354/1‑112 (MQ=37)
                                                                     gggCAGGAAGGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAAGGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGaa                                                                       <  5:118849/149‑1 (MQ=255)
                                                                      ggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAAt                                                                      <  5:176677/149‑1 (MQ=255)
                                                                      ggCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAAt                                                                      >  6:180530/1‑149 (MQ=255)
                                                                        cATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCcgcg                                                                         <  6:192466/144‑1 (MQ=255)
                                                                        cATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGaagat                                                                    >  5:192466/1‑146 (MQ=255)
                                                                        cATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGc                                                                    >  1:266611/1‑149 (MQ=255)
                                                                        cATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGc                                                                    <  3:147280/149‑1 (MQ=255)
                                                                         aTGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGcc                                                                   >  5:281556/1‑149 (MQ=255)
                                                                         aTGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGcc                                                                   >  2:193712/1‑149 (MQ=255)
                                                                         aTGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGGTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGcg                                                                   >  6:241336/1‑148 (MQ=255)
                                                                           gAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCtt                                                                 >  6:290077/1‑149 (MQ=255)
                                                                            aaCGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCtt                                                                 <  3:278678/148‑1 (MQ=255)
                                                                              cGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAg                                                              <  7:41427/149‑1 (MQ=255)
                                                                                 ttCGCCTGAAGGCGTTGCTGCCTACCTGAGCCAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAg                                                              >  7:198636/1‑146 (MQ=255)
                                                                                   cGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAg                                                           <  1:193712/147‑1 (MQ=255)
                                                                                    gCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGcc                                                                                                                           >  5:46661/1‑84 (MQ=255)
                                                                                    gCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGcc                                                                                                                           <  6:46661/84‑1 (MQ=255)
                                                                                      cTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCaa                                                      >  6:93412/1‑149 (MQ=255)
                                                                                           ggCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGtt                                                 <  4:37653/149‑1 (MQ=255)
                                                                                            gCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGaagaggcc                                                                 <  1:114623/132‑7 (MQ=255)
                                                                                            gCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGaagaggcc                                                                 >  2:114623/1‑126 (MQ=255)
                                                                                               ttGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑aaa                                            <  8:116026/149‑1 (MQ=255)
                                                                                                   tgcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑a                                              <  8:290336/143‑1 (MQ=255)
                                                                                                   tgcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑a                                              >  7:290336/1‑143 (MQ=255)
                                                                                                    gcCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATcgcg                                       >  4:169671/1‑149 (MQ=255)
                                                                                                     ccTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATcgcg                                       <  3:209825/148‑1 (MQ=255)
                                                                                                     ccTACCTGAGCGAGAACGAGGGCAAGCTGTAATTGGTGCGTGCATGAAAAAACCGGCCTTCGTGGCCTGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTAAGCAGATCAATCGTAG‑AAATcgcg                                       <  4:215720/148‑1 (MQ=255)
                                                                                                          cTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAgcc                                 >  8:146590/1‑149 (MQ=255)
                                                                                                               cGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCa                            >  2:272031/1‑149 (MQ=255)
                                                                                                                gagaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCAt                           >  1:268121/1‑149 (MQ=255)
                                                                                                                gagaACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAACCGCCCAt                           <  7:156972/149‑1 (MQ=255)
                                                                                                                     cGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGgcgcgc                                                                              <  3:14930/94‑1 (MQ=255)
                                                                                                                     cGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGgcgcgc                                                                              >  4:14930/1‑94 (MQ=255)
                                                                                                                        tGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGAGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCCCGAATGCCTTTAGCACATCAATCGTTG‑AAAACGAGCCAGCCGCCCAatactttc                    >  3:248145/1‑148 (MQ=255)
                                                                                                                               cTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt            <  7:146590/149‑1 (MQ=255)
                                                                                                                               cTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt            <  5:93412/149‑1 (MQ=255)
                                                                                                                                tGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt            >  2:108204/1‑148 (MQ=255)
                                                                                                                                 gTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAg                                                              >  1:491/1‑98 (MQ=255)
                                                                                                                                 gTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAg                                                              <  2:491/98‑1 (MQ=255)
                                                                                                                                  tAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTGAAAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGa          <  1:53988/149‑1 (MQ=255)
                                                                                                                                  tAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGtt            <  1:272031/146‑1 (MQ=255)
                                                                                                                                          gcGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGAt                                                         >  3:57025/1‑94 (MQ=255)
                                                                                                                                          gcGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGAt                                                         <  4:57025/94‑1 (MQ=255)
                                                                                                                                            gTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCTGGCTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCg  >  1:84051/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GGTTGACGCTCTGCAGTTCCACGAAGAGCACGGCGAAGTGTGCCCTGCCAACTGGAAAAAAGGCGACAAGGGCATGAACGCTTCGCCTGAAGGCGTTGCTGCCTACCTGAGCGAGAACGCTGGCAAGCTGTAATTGCTGCGTGCATGAAAAAACCGGCCTTCGTGGCCGGTTTTTTTATGCCTTCCTGTGCCGGCCT‑‑CTTCGCGGGCGCGCCCGCGAATGCCTTTAGCAGATCAATCGTTG‑AAATCGCGCCAGCCGCCCATTTCTTTCCAGCGGTTGACGATGCCG  >  NC_002947/1243579‑1243864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑