| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,275,087 | G→C | 16.0% | intergenic (‑284/+28) | PP_5462 ← / ← PP_5463 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,275,087 | 0 | G | C | 16.0% | 56.1 / 7.0 | 31 | intergenic (‑284/+28) | PP_5462/PP_5463 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (16/10); new base C (3/2); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
CGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCT > NC_002947/1274942‑1275227 | cGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATcgg < 1:117019/148‑1 (MQ=255) aTGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgg < 2:241214/148‑1 (MQ=255) tGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCGGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgggc < 5:39286/149‑1 (MQ=255) tgCATTTTGGTCTATGCGGCGGATAGTGAATGTCGTAGCCTGATTCCCTGTTAAGTGGGCTCCTATAAGGTGCGGGAGAGCGGTTATGATGGGGGTGATGGTTGTAAAGGTGAAAGTAGAATGAGCAAAGCCCGCCCATCGGCGGGCt < 1:195022/147‑1 (MQ=255) gTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAggggggg > 7:102310/1‑149 (MQ=255) aTCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggggggg > 7:201720/1‑146 (MQ=255) gagaGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATTAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGTGCAAAGCCCGCCGATGGGCGGGCTTCTGGGGGGGGCAGAAGGGCtt > 7:258906/1‑148 (MQ=255) gagTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAACCCGCCGATGGGCGGGCTTCAAGGGGGGGCAGAAGGGCTATc > 5:89911/1‑149 (MQ=255) gacgCCTGCATCCCGGTTCCGTCGGCCCCAAAAAGGTGCGGGACCGCGGTACTGATGGGGGTGATGGTGGTAAAGGTGACCGGAGAATGACCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGCGCAGAAGGGCTATCGTACTACGGTc < 2:199623/146‑1 (MQ=255) cggcctCCCGGTTACGGCGGCTCCAATCACGCGCGGGCGAGCGGTAATGATGGGGGCGATGGGGGTAAAGGGCACAGGAGAATGAGCACAGCCCGCCGCGGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTtc < 8:201720/144‑1 (MQ=255) cTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAGGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGGGATCGTACTACGGTCGcg > 1:301454/1‑146 (MQ=255) ggTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCcccc > 7:45344/1‑149 (MQ=255) gTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGACGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAgg > 7:195628/1‑149 (MQ=255) tGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAcccgccc > 7:95189/1‑149 (MQ=255) cgagCGGTAAGGAGGGGGGTCATCGTGGCAAAGGTGAAAGGACACTGAGCAACCCCCGCCGCTGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCActc < 8:102310/148‑1 (MQ=255) gTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCtcat > 1:261231/1‑148 (MQ=255) aaggaggggggTACTGGGGGGAAAGGTCAAAGGAGAATGAGCACAGCCCGCCGATGGGCGGGCTTCAGGGGGTGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCtcatca < 8:195628/142‑1 (MQ=255) ggggtctgggggTAAAGGTGAACGGACAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCa < 6:89911/143‑1 (MQ=255) gATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACttt > 6:253483/1‑149 (MQ=255) gtAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGa > 2:267430/1‑149 (MQ=255) gTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCt > 2:178851/1‑149 (MQ=255) gAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCttt > 7:8483/1‑149 (MQ=255) agaATGACCAAAGCCCGCCGCTGGGCGGGCTTCAGGGGGCCGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATaa < 8:95189/149‑1 (MQ=255) aTGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGtt < 5:253483/149‑1 (MQ=255) tGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTg > 5:65053/1‑149 (MQ=255) tgaaGCCCGCCCATCGGCGGGCTTTAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATc > 3:175550/3‑149 (MQ=255) cAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTAtt < 1:267430/149‑2 (MQ=255) aaGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCa < 8:8483/149‑1 (MQ=255) gcccgcccATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTCCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCAct < 6:236760/95‑1 (MQ=255) gcccgcccATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTCCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCAct > 5:236760/1‑95 (MQ=255) cccGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGcc < 6:65053/149‑1 (MQ=255) ccgccgATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCt > 6:131655/1‑149 (MQ=255) ccgccgATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACGTTCa > 6:252748/1‑116 (MQ=255) ccgcccATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCCCCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGc > 7:19715/1‑147 (MQ=255) | CGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCT > NC_002947/1274942‑1275227 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |