Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,342,252 A→T 16.7% intergenic (‑36/+19) PP_1166 ← / ← PP_1167 DMT superfamily permease/TRAP dicarboxylate transporter subunit DctM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,342,2520AT16.7% 88.2 / 11.7 42intergenic (‑36/+19)PP_1166/PP_1167DMT superfamily permease/TRAP dicarboxylate transporter subunit DctM
Reads supporting (aligned to +/- strand):  ref base A (19/16);  new base T (3/4);  total (22/20)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01

CGCGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGC  >  NC_002947/1342115‑1342395
                                                                                                                                         |                                                                                                                                               
cgcgGCGATGTGTGTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGcccctg                                                                                                                                      <  3:4/149‑3 (MQ=255)
cgcgGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGcccctg                                                                                                                                      <  5:309306/149‑3 (MQ=255)
  cggcgTAGTGGTTGCCAGCGAATGAACAGGCCACGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGCTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGt                                                                                                                                    <  6:167953/149‑1 (MQ=255)
  cggcgATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGt                                                                                                                                    <  4:162727/149‑1 (MQ=255)
    gcgATGTGGTTGCCAGCGAATTAACTGGCCAGGAGGGCGAGCTGGGCGATGGCTAGTTTGCGGGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTCCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCa                                                                                                                                  <  6:56213/149‑1 (MQ=255)
            gTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTa                                                                                                                            >  1:77232/1‑147 (MQ=255)
            gTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACa                                                                                                                          <  8:225128/149‑1 (MQ=255)
               gCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGc                                                                                                                        <  3:17677/148‑1 (MQ=255)
                       aTGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGTTACAGCCGAGGAGGGAACAGCCATGTGCAGGACCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTa                                                                                                               <  4:66057/149‑1 (MQ=255)
                         gAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCGGGGGCCGCTTGGCGGCCCCTggg                                                                                                                                    >  6:194307/1‑125 (MQ=255)
                         gAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCGGGGGCCGCTTGGCGGCCCCTggg                                                                                                                                    <  5:194307/126‑2 (MQ=255)
                          aaCAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAgg                                                                                                             >  2:59879/1‑148 (MQ=255)
                              ggCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGc                                                                                                         >  5:305387/1‑148 (MQ=255)
                                          gAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGG‑CCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGc                                                                                           >  2:263957/1‑149 (MQ=255)
                                             catcgCGATGGCTAGTTTGCGTGGGAAACGCGTTCCAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGACCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTg                                                                                         <  8:233466/145‑1 (MQ=255)
                                              aGGGCGATGGCTAGTTTGCGTGGGAAACGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTgg                                                                                        <  3:58200/149‑1 (MQ=255)
                                                ggCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGCCCCTgg                                                                                                                                     <  7:148643/102‑1 (MQ=255)
                                                ggCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGCCCCTgg                                                                                                                                     >  8:148643/1‑102 (MQ=255)
                                                           gTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACt                                                                           >  5:293811/1‑149 (MQ=255)
                                                                           cGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTgg                                                                                        >  8:278581/1‑120 (MQ=255)
                                                                           cGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTgg                                                                                        <  7:278581/120‑1 (MQ=255)
                                                                           cGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACAACGCTAGGCAGCCACAGCCAGATCGCTGTAATGTAAGTCACTGCCATCAGCACCAgg                                                            >  1:50005/1‑148 (MQ=255)
                                                                                    cGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCa                                                  <  2:77232/149‑1 (MQ=255)
                                                                                              aCAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAAc                                          <  7:23875/147‑1 (MQ=255)
                                                                                                ctccATGTGCAGGATCCATTTCCATCAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGc                                       <  2:270586/146‑1 (MQ=255)
                                                                                                aGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCa                                      >  2:188408/1‑149 (MQ=255)
                                                                                                  ccATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCAt                                     >  5:251731/1‑148 (MQ=255)
                                                                                                   cATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGc                                       >  1:270586/1‑145 (MQ=255)
                                                                                                       tgCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAgcgc                               <  1:59879/149‑1 (MQ=255)
                                                                                                        gCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCCGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGcc                              >  2:98958/1‑149 (MQ=255)
                                                                                                          aGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCtt                            >  7:218110/1‑149 (MQ=255)
                                                                                                                 aTTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCtt                            <  6:250019/142‑1 (MQ=255)
                                                                                                                 aTTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCtt                            >  5:250019/1‑142 (MQ=255)
                                                                                                                     gCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGAc                  <  8:218110/148‑1 (MQ=255)
                                                                                                                       aaGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTc                >  2:83125/1‑148 (MQ=255)
                                                                                                                       aaGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGCAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTACAACGGCATCAGCGCCTTCACCGTCGACTc                >  3:253369/1‑148 (MQ=255)
                                                                                                                           gCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATAc           >  7:291415/1‑149 (MQ=255)
                                                                                                                              cgggggCCGCTTGGCGGCCCCTGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCa        >  4:236233/3‑149 (MQ=255)
                                                                                                                               aGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCAc       >  3:286102/1‑149 (MQ=255)
                                                                                                                                  ggacgccAAGCTGCACTCGTTCATAATTACAGCAACACTCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCAGTTCCATCAGAACCAGGATCAGCGCCAGGTATAAAGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCtt    <  2:50005/146‑1 (MQ=255)
                                                                                                                                   gccgccAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTg   >  4:274761/1‑149 (MQ=255)
                                                                                                                                    ccgccAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGc  >  4:224352/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                               
CGCGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGC  >  NC_002947/1342115‑1342395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑