Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,342,262 C→T 14.6% intergenic (‑46/+9) PP_1166 ← / ← PP_1167 DMT superfamily permease/TRAP dicarboxylate transporter subunit DctM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,342,2620CT14.6% 95.9 / 8.0 41intergenic (‑46/+9)PP_1166/PP_1167DMT superfamily permease/TRAP dicarboxylate transporter subunit DctM
Reads supporting (aligned to +/- strand):  ref base C (19/16);  new base T (3/3);  total (22/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGCCAATGGCCGAGC  >  NC_002947/1342117‑1342407
                                                                                                                                                 |                                                                                                                                                 
cggcgTAGTGGTTGCCAGCGAATGAACAGGCCACGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGCTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGt                                                                                                                                                <  6:167953/149‑1 (MQ=255)
cggcgATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGt                                                                                                                                                <  4:162727/149‑1 (MQ=255)
  gcgATGTGGTTGCCAGCGAATTAACTGGCCAGGAGGGCGAGCTGGGCGATGGCTAGTTTGCGGGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTCCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCa                                                                                                                                              <  6:56213/149‑1 (MQ=255)
          gTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTa                                                                                                                                        >  1:77232/1‑147 (MQ=255)
          gTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACa                                                                                                                                      <  8:225128/149‑1 (MQ=255)
             gCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGc                                                                                                                                    <  3:17677/148‑1 (MQ=255)
                     aTGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGTTACAGCCGAGGAGGGAACAGCCATGTGCAGGACCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTa                                                                                                                           <  4:66057/149‑1 (MQ=255)
                       gAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCGGGGGCCGCTTGGCGGCCCCTggg                                                                                                                                                >  6:194307/1‑125 (MQ=255)
                       gAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCGGGGGCCGCTTGGCGGCCCCTggg                                                                                                                                                <  5:194307/126‑2 (MQ=255)
                        aaCAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAgg                                                                                                                         >  2:59879/1‑148 (MQ=255)
                            ggCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGc                                                                                                                     >  5:305387/1‑148 (MQ=255)
                                        gAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGG‑CCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGc                                                                                                       >  2:263957/1‑149 (MQ=255)
                                           catcgCGATGGCTAGTTTGCGTGGGAAACGCGTTCCAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGACCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTg                                                                                                     <  8:233466/145‑1 (MQ=255)
                                            aGGGCGATGGCTAGTTTGCGTGGGAAACGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTgg                                                                                                    <  3:58200/149‑1 (MQ=255)
                                              ggCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGCCCCTgg                                                                                                                                                 >  8:148643/1‑102 (MQ=255)
                                              ggCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCTTGGCGGCCCCTgg                                                                                                                                                 <  7:148643/102‑1 (MQ=255)
                                                         gTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACt                                                                                       >  5:293811/1‑149 (MQ=255)
                                                                         cGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTgg                                                                                                    <  7:278581/120‑1 (MQ=255)
                                                                         cGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTgg                                                                                                    >  8:278581/1‑120 (MQ=255)
                                                                         cGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACAACGCTAGGCAGCCACAGCCAGATCGCTGTAATGTAAGTCACTGCCATCAGCACCAgg                                                                        >  1:50005/1‑148 (MQ=255)
                                                                                  cGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCa                                                              <  2:77232/149‑1 (MQ=255)
                                                                                            aCAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAAc                                                      <  7:23875/147‑1 (MQ=255)
                                                                                              ctccATGTGCAGGATCCATTTCCATCAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGc                                                   <  2:270586/146‑1 (MQ=255)
                                                                                              aGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCa                                                  >  2:188408/1‑149 (MQ=255)
                                                                                                ccATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCAt                                                 >  5:251731/1‑148 (MQ=255)
                                                                                                 cATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGc                                                   >  1:270586/1‑145 (MQ=255)
                                                                                                     tgCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAgcgc                                           <  1:59879/149‑1 (MQ=255)
                                                                                                      gCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCCGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGcc                                          >  2:98958/1‑149 (MQ=255)
                                                                                                        aGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCtt                                        >  7:218110/1‑149 (MQ=255)
                                                                                                               aTTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCtt                                        >  5:250019/1‑142 (MQ=255)
                                                                                                               aTTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCtt                                        <  6:250019/142‑1 (MQ=255)
                                                                                                                   gCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGAc                              <  8:218110/148‑1 (MQ=255)
                                                                                                                     aaGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTc                            >  2:83125/1‑148 (MQ=255)
                                                                                                                     aaGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGCAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTACAACGGCATCAGCGCCTTCACCGTCGACTc                            >  3:253369/1‑148 (MQ=255)
                                                                                                                         gCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATAc                       >  7:291415/1‑149 (MQ=255)
                                                                                                                            cgggggCCGCTTGGCGGCCCCTGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCa                    >  4:236233/3‑149 (MQ=255)
                                                                                                                             aGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCAc                   >  3:286102/1‑149 (MQ=255)
                                                                                                                                ggacgccAAGCTGCACTCGTTCATAATTACAGCAACACTCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCAGTTCCATCAGAACCAGGATCAGCGCCAGGTATAAAGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCtt                <  2:50005/146‑1 (MQ=255)
                                                                                                                                 gccgccAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTg               >  4:274761/1‑149 (MQ=255)
                                                                                                                                  ccgcttggCGGCCCCTGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGc              <  3:236233/142‑1 (MQ=255)
                                                                                                                                  ccgccAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGc              >  4:224352/1‑149 (MQ=255)
                                                                                                                                             cccccGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGCCAATGGCCGAg   <  8:291415/149‑1 (MQ=255)
                                                                                                                                              ccccGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCAc                   >  4:22926/1‑132 (MQ=255)
                                                                                                                                              ccccGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCAc                   <  3:22926/132‑1 (MQ=255)
                                                                                                                                              ccccGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGCCAATGGCCGAGc  <  3:224352/149‑1 (MQ=255)
                                                                                                                                                ccGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCg                                <  2:65748/117‑1 (MQ=255)
                                                                                                                                                ccGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCg                                >  1:65748/1‑117 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGGCGATGTGGTTGCCAGCGAATGAACAGGCCAGGAGGGCGAGCAGGGCGATGGCTAGTTTGCGTGGGAAAAGCGGTACAGCCGAGGAGGGAACAGCCATGTGCAGGATCCATTTGCAAGAGCCCAGGGGCCGCCAAGCGGCCCCCGGTCATAATTACAGCACCACGCTAGGCAGCCACAGCGAGATCGCTGGAATGTAAGTCACTGCCATCAGCACCAGGAACAGCGCCAGGTAGAACGGCATCAGCGCCTTCACCGTCGACTCGATACTCACCTTGCCAATGGCCGAGC  >  NC_002947/1342117‑1342407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑