| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,356,405 | G→C | 8.5% | G94A (GGC→GCC) | PP_1181 → | two‑component system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,356,405 | 0 | G | C | 8.5% | 176.6 / 6.5 | 59 | G94A (GGC→GCC) | PP_1181 | two‑component system response regulator |
| Reads supporting (aligned to +/- strand): ref base G (29/25); new base C (3/2); total (32/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.19e-01 | |||||||||||
TCATCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGACAACCG > NC_002947/1356258‑1356552 | tcatcGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGc > 8:140836/1‑149 (MQ=255) gACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTgg < 3:262394/148‑1 (MQ=255) gACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGa < 7:133633/149‑1 (MQ=255) ccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTgg < 6:53349/149‑1 (MQ=255) ccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTgg > 1:298572/1‑149 (MQ=255) agccCGACCTGGTGATCCTCGACCTGATGCTCCCCGTCGAGGACGGCCTGCGCATCTGCCGCCGGGTGCGCCGCCAGTACAAGGGGCCGATCCTAATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCATGGACCTGGgc > 7:177555/1‑149 (MQ=255) cccGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGAc > 4:102344/1‑141 (MQ=255) cccGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGAc < 3:102344/141‑1 (MQ=255) ccGACCTGGTGATCCTCGACCTGATGCTCCCCGACGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGcc > 1:39885/1‑149 (MQ=255) gATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTgg < 1:106535/134‑1 (MQ=255) gATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGCCCTGGACCTgg > 2:106535/1‑134 (MQ=255) tCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTg > 5:300279/1‑148 (MQ=255) cTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAcc > 3:226048/1‑149 (MQ=255) aTGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGCCCTGGGCGCCGACGACTACGTCTGCAAACCCGt > 7:218583/1‑149 (MQ=255) gCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTgc > 7:86912/1‑149 (MQ=255) tCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcg < 5:275449/123‑1 (MQ=255) tCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcg > 6:275449/1‑123 (MQ=255) aGGACGGCGATAGCATCTGCCGCGGGGTGCGCAGCCAGTACCCGGGGCCGCTCCTCATGCTCACCGCGCGCAGTGATGACCTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTG < 8:218583‑M1/149‑1 (MQ=255) ggACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTT < 8:280214‑M1/147‑1 (MQ=255) ggCCTGAGCATCTGCCGCCGGGTGCGCAGCCTGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGt < 2:163067/119‑1 (MQ=255) gCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGtc > 4:228683‑M1/1‑146 (MQ=255) ccTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTgg < 3:104933/149‑1 (MQ=255) cTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCCGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGc > 8:158392/1‑149 (MQ=255) aGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGTTCCTCATGCTCACCGCGCTCTGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc < 8:177555/148‑1 (MQ=255) aTCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGGTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTAt > 1:215838/1‑149 (MQ=255) tGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATc > 5:112384/1‑147 (MQ=255) tGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGCGCGCCCACGCTTgctgct < 1:302302/137‑1 (MQ=255) tGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGAGCGCCCACGCTTgctgct > 2:302302/1‑137 (MQ=255) ccgccgGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATc < 2:39885/145‑1 (MQ=255) ccgccgGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAgg < 8:172033/149‑1 (MQ=255) ggccgGGTGCGCAGCCATTACCAGGGGCCGATCCTAATGCGCACCGCACGCAGGGATGAACTGGACCCGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGc < 7:202387/148‑1 (MQ=255) cAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcgac > 1:116971/1‑85 (MQ=255) cAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCcgac < 2:116971/85‑1 (MQ=255) aGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGcgccg > 8:273403/1‑149 (MQ=255) ccAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTG > 4:174942‑M1/1‑115 (MQ=255) ccAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGaaa < 7:140836/149‑1 (MQ=255) gggCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCACACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGt > 8:93042/1‑149 (MQ=255) ggCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTg > 7:73542/1‑149 (MQ=255) tCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGAcagc > 6:300279‑M2/1‑145 (MQ=255) cTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGaaa < 3:144536/136‑1 (MQ=255) cTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGaaa > 4:144536/1‑136 (MQ=255) cATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGAcagcaagc < 1:237126‑M2/149‑9 (MQ=255) aTGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGAcagcaagc > 7:273403‑M2/1‑140 (MQ=255) tGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAgcgc < 2:237126/149‑1 (MQ=255) gCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCCCGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAgcgc > 7:181179/1‑148 (MQ=255) cTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGtccacggtttcgctgcggcgcagcagggcctggatacgtgccagcagcaagcgtg < 3:228683‑M1/148‑56 (MQ=255) ccGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGtccacggtttcgctgcggcgcagcagggcctggatacgtgccagcagcaagcgtgg < 8:89741‑M1/145‑57 (MQ=255) ccGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGtccacggtttcgctgcggcgcagcagggcctggatacgtgccagcagcaagcgtgg > 7:89741‑M1/1‑89 (MQ=255) gcgcAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTgg < 2:298572/149‑1 (MQ=255) aGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCtt < 8:86912/149‑1 (MQ=255) aCTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTgcgc > 2:31837/1‑149 (MQ=255) aCTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTgcgc > 6:15044/1‑149 (MQ=255) cTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCt > 8:146051/1‑149 (MQ=255) ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCt < 2:290524/129‑1 (MQ=255) ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCt > 1:290524/1‑129 (MQ=255) ccAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCCGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTa > 6:186311/1‑149 (MQ=255) aGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATc < 1:277178/149‑1 (MQ=255) tCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCg < 6:208047/132‑1 (MQ=255) tCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCg > 5:208047/1‑132 (MQ=255) ggCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGACAACCg > 7:166832/1‑149 (MQ=255) | TCATCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATCGACAACCG > NC_002947/1356258‑1356552 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |