Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,364,058 G→T 6.7% V251L (GTG→TTG)  dctA‑I → C4‑dicarboxylate transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,364,0580GT 6.7% 188.6 / 3.7 60V251L (GTG→TTG) dctA‑IC4‑dicarboxylate transport protein
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base T (2/2);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01

GCTTTCGGCGCCATGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACT  >  NC_002947/1363911‑1364201
                                                                                                                                                   |                                                                                                                                               
gccgaaagcgcCATGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGt                                                                                                                                                <  3:12396/142‑1 (MQ=255)
  tttCGGCGCCATGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGa                                                                                                                                              >  4:128298/1‑149 (MQ=255)
            aTGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGAt                                                                                                                                    >  2:221162/1‑149 (MQ=255)
                ccTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaac                                                                                                                                  >  4:234409/1‑144 (MQ=255)
                ccTTCACCAATGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaac                                                                                                                                  <  3:234409/147‑4 (MQ=255)
                   tCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTa                                                                                                                              >  2:75641/1‑148 (MQ=255)
                    cACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACa                                                                                                                            >  6:260385/1‑149 (MQ=255)
                           ggCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTg                                                                                                                      >  6:260546/1‑148 (MQ=255)
                            gCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTgaa                                                                                                                    <  1:109661/149‑1 (MQ=255)
                              cAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaaccg                                                                                                                                >  8:256683/1‑130 (MQ=255)
                              cAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCTTGATCACGCTGaaaccg                                                                                                                                <  7:256683/135‑6 (MQ=255)
                              cAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTgaag                                                                                                                   >  4:76790/1‑148 (MQ=255)
                                   tGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAActg                                                                                                              <  6:247062/148‑1 (MQ=255)
                                    ggTGTTGGTTCGCGGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAActgc                                                                                                             <  8:214909/148‑1 (MQ=255)
                                          ggTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGt                                                                                                      >  7:232823/1‑149 (MQ=255)
                                          ggTTCGCTGGTGCAGCTGGGCTACCTGATGGCCAGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGt                                                                                                      >  1:39737/1‑149 (MQ=255)
                                           gTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTg                                                                                                     <  3:68262/149‑1 (MQ=255)
                                               gCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTgg                                                                                                 <  8:232823/149‑1 (MQ=255)
                                               gCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTgg                                                                                                 <  2:66162/149‑1 (MQ=255)
                                               gCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTgg                                                                                                 >  1:66162/1‑149 (MQ=255)
                                                 tGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTggg                                                                                                >  1:264939/1‑148 (MQ=255)
                                                 tGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGt                                                                                               <  8:195635/149‑1 (MQ=255)
                                                         cTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTc                                                                                       >  1:101648/1‑149 (MQ=255)
                                                            ggCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTcc                                                                                      <  1:221162/147‑1 (MQ=255)
                                                             gCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCg                                                                                   <  2:101648/149‑1 (MQ=255)
                                                              cTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCg                                                                                   >  2:272023/1‑148 (MQ=255)
                                                                    gATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCgg                                                                            >  6:150818/1‑149 (MQ=255)
                                                                         ccTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTg                                                                       >  1:238997/1‑149 (MQ=255)
                                                                              ttCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACg                                                                  >  7:239854/1‑149 (MQ=255)
                                                                               tCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCt                                                                                                   >  5:212332/1‑115 (MQ=255)
                                                                               tCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCt                                                                                                   <  6:212332/115‑1 (MQ=255)
                                                                                cTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGc                                                                 <  5:260546/148‑1 (MQ=255)
                                                                                   cATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCa                                                                <  1:269238/146‑1 (MQ=255)
                                                                                   cATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGCCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTCCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGc                                                             >  6:52174/1‑149 (MQ=255)
                                                                                      cACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTgg                                                          >  4:293128/1‑149 (MQ=255)
                                                                                      cACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTgg                                                          >  1:41450/1‑149 (MQ=255)
                                                                                       aCCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGGGTCGGCCCTGCCAc                                                                   >  1:56314/1‑139 (MQ=255)
                                                                                       aCCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGGGTCGGCCCTGCCAc                                                                   <  2:56314/139‑1 (MQ=255)
                                                                                                ctgTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATgg                                                 <  3:128298/148‑1 (MQ=255)
                                                                                                      gTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCt                                          >  2:25973/1‑149 (MQ=255)
                                                                                                         cTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTggg                                       <  5:150818/149‑1 (MQ=255)
                                                                                                          tggtggTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGt                                      <  8:239854/149‑1 (MQ=255)
                                                                                                            gtggtgCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGt                                      <  5:260385/147‑1 (MQ=255)
                                                                                                             tggtgCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGcc                                   <  3:293128/149‑1 (MQ=255)
                                                                                                               gtgCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCaa                                 <  8:174160/149‑1 (MQ=255)
                                                                                                                  cTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGa                               <  1:272023/148‑1 (MQ=255)
                                                                                                                     ggCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAAt                            <  2:238997/148‑1 (MQ=255)
                                                                                                                       cGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCgg                         <  3:76790/149‑1 (MQ=255)
                                                                                                                           aTCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAg                                <  3:115986/138‑1 (MQ=255)
                                                                                                                              tGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCgg                         <  2:89888/142‑1 (MQ=255)
                                                                                                                              tGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCgg                         >  1:89888/1‑142 (MQ=255)
                                                                                                                              tGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGAGCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCCTGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTggg                  >  5:119572/1‑149 (MQ=255)
                                                                                                                                        aCGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGAATGCTGGACAAGATTGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTTTc        >  8:72020/1‑149 (MQ=255)
                                                                                                                                        aCGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGAGCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATc        <  8:22439/149‑1 (MQ=255)
                                                                                                                                         cGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTTAATAACTGCTGATCGTGATTGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTATTGGGCCTGGTTATcc       <  5:52174/149‑1 (MQ=255)
                                                                                                                                          ggTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATccc      <  2:41450/149‑1 (MQ=255)
                                                                                                                                            tttCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCg     >  6:124241/1‑148 (MQ=255)
                                                                                                                                               cAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACt  <  4:76743/148‑1 (MQ=255)
                                                                                                                                               cAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACt  >  3:76743/1‑148 (MQ=255)
                                                                                                                                               cAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACt  <  2:39737/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GCTTTCGGCGCCATGGCCTTCACCATTGGCCAGTATGGTGTTGGTTCGCTGGTGCAGCTGGGCTACCTGATGGCCTGCTTCTACATCACCTGCCTGCTGTTCGTGCTGGTGGTGCTGGGCGGTATCTGCCGCGCCCACGGTTTCAGCGTGATCAAGCTGATCCGCTACATCCGTGAAGAACTGCTGATCGTGCTGGGTACTTCGTCCTCGGAGTCGGCCCTGCCACGCATGCTGGCCAAGATGGAGCGCCTGGGTGCCAAGAAATCGGTAGTGGGCCTGGTTATCCCGACT  >  NC_002947/1363911‑1364201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑